A5032185321- Phytochemicals and Antioxidant Activities
- Antioxidant Activity and Oxidative Stress
- Glycosylation and Glycoproteins Research
- Natural product bioactivities and synthesis
- S100 Proteins and Annexins
- Atherosclerosis and Cardiovascular Diseases
- Phytochemistry and Biological Activities
- Neonatal Respiratory Health Research
- Diet, Metabolism, and Disease
- Genomics and Phylogenetic Studies
- Retinoids in leukemia and cellular processes
- Pharmacological Effects of Natural Compounds
- Trace Elements in Health
- Invertebrate Immune Response Mechanisms
- Vitamin K Research Studies
- Adipose Tissue and Metabolism
- Bioactive natural compounds
- Photosynthetic Processes and Mechanisms
- Traditional Chinese Medicine Analysis
- Medicinal Plants and Neuroprotection
- Neurological Disease Mechanisms and Treatments
- Pharmacology and Obesity Treatment
- Eicosanoids and Hypertension Pharmacology
- Sesquiterpenes and Asteraceae Studies
- Mitochondrial Function and Pathology
Boiron (France)
2022-2025
Quadram Institute
2007-2018
Norwich Research Park
2007-2018
Abstract Ruminococcus gnavus is a human gut symbiont wherein the ability to degrade mucins mediated by an intramolecular trans -sialidase ( Rg NanH). NanH comprises GH33 catalytic domain and sialic acid-binding carbohydrate-binding module (CBM40). Here we used glycan arrays, STD NMR, X-ray crystallography, mutagenesis binding assays determine structure function of NanH_CBM40 CBM40). CBM40 displays canonical β-sandwich fold broad specificity towards sialoglycans with millimolar affinity α2,3-...
Mitochondria mediate most of the energy production that occurs in majority eukaryotic organisms. These subcellular organelles contain a genome differs from nuclear and is referred to as mitochondrial DNA (mtDNA). Despite disparity gene content, all mtDNAs encode at least two components electron transport chain, including cytochrome c oxidase I (Cox1). A positionally conserved ORF has been found on complementary strand cox1 genes both mitochondria (protist, plant, fungal animal)...
Anas barbariae 200K, a homeopathic medicine, is traditionally used for influenza-like illnesses. We investigated the effects of 200K on microglial cells, subpopulation macrophages specific to central nervous system often study inflammatory processes and oxidative stress generated during episodes. The demonstrates effect cell stiffness reactive oxygen species production using atomic force microscopy fluorescence techniques, respectively. Our results showed that rapidly increased in resting...
Quercetin is a major flavonoid that contributes to the reduced risk of cardiovascular disease associated with dietary ingestion fruits and vegetables. We have pharmacologically characterized effect quercetin, its sulphate glucuronide metabolites, on vasoconstrictor vasodilator responses in porcine isolated coronary artery.Segments artery were prepared for either isometric tension recording or determination cyclic GMP content. The quercetin metabolites submaximal U46619 was examined presence...
The consumption of flavan-3-ols has been associated with reduced risk cardiovascular diseases and improvements in vascular function. However, the nature responsible mechanisms underlying responses are not fully understood. We used microarrays to search for molecular changes response exposure (-)-epicatechin (EC), procyanidin dimer B2, a mixture oligomeric procyanidins human umbilical vein endothelial cells (HUVECs). No gene expression were detected HUVECs exposed EC or however, induced...
Intestinal mucins trigger immune responses upon recognition by dendritic cells via protein-carbohydrate interactions. We used a combination of structural, biochemical, biophysical, and cell-based approaches to decipher the specificity interaction between mucin glycans mammalian lectins expressed in gut, including galectin (Gal)-3 C-type lectin receptors. Gal-3 differentially recognized intestinal with different O-glycosylation profiles, as determined mass spectrometry (MS). Modification...
The plant Arnica montana L. has been shown to alleviate inflammation, pain and swelling associated with trauma, post-operative clinical conditions, yet the mechanism of action is not well understood.The study was designed investigate effect (A. montana) mother tincture homeopathic dilutions on inflammation markers, oxidative stress cell migration in diverse culture models.We tested A. a range different human murine models demonstrate their anti-inflammatory properties by measuring...
BACKGROUND AND PURPOSE Quercetin is anti‐inflammatory in macrophages by inhibiting lipopolysaccharide (LPS)‐mediated increases cytokine and nitric oxide production but there little information regarding the corresponding effect on vasculature. We have examined of quercetin, its principal human metabolites, inflammatory changes porcine isolated coronary artery. EXPERIMENTAL APPROACH Porcine artery segments were incubated overnight at 37°C modified Krebs‐Henseleit solution with or without 1...
The major dietary flavonol quercetin, which has been shown to improve endothelial function and decrease blood pressure, is extensively metabolized during absorption. This study examined the relative abilities of quercetin its human metabolites modulate expression eNOS ET-1, are involved in regulating homeostasis. Quercetin aglycone significantly reduced both protein gene HUVEC, mirroring effects pro-inflammatory cytokine TNFα. In presence TNFα caused further reductions eNOS, whereas were...
There is accumulating evidence from epidemiological and human intervention studies that quercetin-rich diets can protect against cardiovascular diseases. Quercetin glycosides are modified during metabolism, the forms reaching systemic circulation glucuronidated, sulfated, methylated. The aim of this study was to analyse potential beneficial effects quercetin its conjugated metabolites on vascular function a co-culture model umbilical artery smooth muscle cells vein endothelial cells. We...
Microglial cells play important roles in inflammatory responses. The level of oxidative stress is a well-known marker inflammation. Homeopathic medicines are often used clinically to alleviate We evaluated the anti-oxidative effect high dilutions Arnica montana (Arnica m.), Arsenicum album (Arsenicum a.), and Lachesis mutus (Lachesis m.) on production reactive oxygen species (ROS) inflamed microglial vitro. cells, exposure lipopolysaccharide (LPS), have induced ROS compared with resting...
Naturally occurring 2,7-anhydro-alpha-N-acetylneuraminic acid (2,7-anhydro-Neu5Ac) is a transglycosylation product of bacterial intramolecular trans-sialidases (IT-sialidases). A facile one-pot two-enzyme approach has been established for the synthesis 2,7-anhydro-sialic derivatives including those containing different sialic forms such as Neu5Ac and N-glycolylneuraminic (Neu5Gc). The based on use Ruminoccocus gnavus IT-sialidase release from glycoproteins, conversion free by aldolase. This...
Background: The level of oxidative stress is a known marker inflammation. Microglial cells are macrophages present in the central nervous system and play important roles inflammatory responses making microglial powerful model for studying Homeopathic medicines often used to alleviate inflammation clinical practice Oscillococcinum® indicated treatment flu-like symptoms. However, date, mechanism action unknown. anti-oxidative effect homeopathic dilutions Arnica montana, Arsenicum album,...
Background: Some homeopathic medicines are traditionally used in medical practice to strengthen immunity and reduce inflammation. In particular, Influenzinum is clinical for the prevention of influenza-like illness, management possible symptoms following an anti-influenza vaccination post-infectious convalescence. Echinacea angustifolia best known its beneficial effects on immune system infectious syndrome. During infection (viral or bacterial), innate first body response. It immediate...
The expression and the subcellular localizations of annexins I, II, IV, VI, XIII in renal epithelial cells were investigated, using immunological techniques with specific monoclonal antibodies. Upon performing Western blotting experiments, no VI detected kidney, whereas IV were. Immunofluorescence labelling procedure performed on thin frozen sections showed presence these three along plasma membrane collecting duct a restricted annexin I at principal cells. Annexin was also found present...
The expression and the subcellular localizations of annexins I, II, IV, VI, XIII in renal epithelial cells were investigated, using immunological techniques with specific monoclonal antibodies. Upon performing Western blotting experiments, no VI detected kidney, whereas IV were. Immunofluorescence labelling procedure performed on thin frozen sections showed presence these three along plasma membrane collecting duct a restricted annexin I at principal cells. Annexin was also found present...