A5021307435- Genomics and Phylogenetic Studies
- Glycosylation and Glycoproteins Research
- Gut microbiota and health
- Research on Leishmaniasis Studies
- Probiotics and Fermented Foods
- SARS-CoV-2 and COVID-19 Research
- Bacteriophages and microbial interactions
- Animal Virus Infections Studies
- Magnetism in coordination complexes
- Organometallic Complex Synthesis and Catalysis
- Trypanosoma species research and implications
- Clostridium difficile and Clostridium perfringens research
- Metal complexes synthesis and properties
- Liver Diseases and Immunity
- Inorganic and Organometallic Chemistry
- Photosynthetic Processes and Mechanisms
- Bacterial Genetics and Biotechnology
- Biochemical and Molecular Research
- Enzyme Structure and Function
- Protein Kinase Regulation and GTPase Signaling
- Plant Stress Responses and Tolerance
- COVID-19 epidemiological studies
- Gallbladder and Bile Duct Disorders
- Nanomaterials for catalytic reactions
- Plant Micronutrient Interactions and Effects
Quadram Institute
2017-2024
Norwich Research Park
2015-2024
The London College
2023
Imperial College London
2023
Applied Graphene Materials (United Kingdom)
2021
University of Edinburgh
2020
Discovery Laboratories (United States)
2018
John Innes Centre
2015
Case Western Reserve University
2007
University of Oxford
1997
We present CoronaHiT, a platform and throughput flexible method for sequencing SARS-CoV-2 genomes (≤ 96 on MinION or > Illumina NextSeq) depending changing requirements experienced during the pandemic. CoronaHiT uses transposase-based library preparation of ARTIC PCR products. Method performance was demonstrated by 2 plates containing 95 59 nanopore platforms comparing to LoCost method. Of 154 samples sequenced using all 3 methods, ≥ 90% genome coverage obtained 64.3% LoCost, 71.4%...
Abstract Ruminococcus gnavus is a human gut symbiont wherein the ability to degrade mucins mediated by an intramolecular trans -sialidase ( Rg NanH). NanH comprises GH33 catalytic domain and sialic acid-binding carbohydrate-binding module (CBM40). Here we used glycan arrays, STD NMR, X-ray crystallography, mutagenesis binding assays determine structure function of NanH_CBM40 CBM40). CBM40 displays canonical β-sandwich fold broad specificity towards sialoglycans with millimolar affinity α2,3-...
The COVID-19 pandemic has spread rapidly throughout the world. In UK, initial peak was in April 2020; county of Norfolk (UK) and surrounding areas, which a stable, low-density population, over 3200 cases were reported between March August 2020. As part activities national Genomics Consortium (COG-UK) we undertook whole genome sequencing SARS-CoV-2 genomes present positive clinical samples from region. These collected by four major hospitals, multiple minor care facilities community...
The human gut symbiont Ruminococcus gnavus scavenges host-derived N-acetylneuraminic acid (Neu5Ac) from mucins by converting it to 2,7-anhydro-Neu5Ac. We previously showed that 2,7-anhydro-Neu5Ac is transported into R. ATCC 29149 before being converted back Neu5Ac for further metabolic processing. However, the molecular mechanism leading conversion of remained elusive. Using 1D and 2D NMR, we elucidated multistep enzymatic oxidoreductase (RgNanOx) leads reversible through formation a...
Located at the tip of cell surface glycoconjugates, sialic acids are forefront host–microbe interactions and, being easily liberated by sialidase enzymes, used as metabolites numerous bacteria, particularly pathogens and commensals living on or near diverse mucosal surfaces. These bacteria rely specific transporters for acquisition host-derived acids. Here, we present first comprehensive genomic phylogenetic analysis bacterial acid transporters, leading to identification multiple new...
Abstract The COVID-19 pandemic has spread rapidly throughout the world. In UK, initial peak was in April 2020; county of Norfolk (UK) and surrounding areas, which a stable, low-density population, over 3,200 cases were reported between March August 2020. As part activities national Genomics Consortium (COG-UK) we undertook whole genome sequencing SARS-CoV-2 genomes present positive clinical samples from region. These collected by four major hospitals, multiple minor care facilities community...
Abstract The COVID-19 pandemic has spread to almost every country in the world since it started China late 2019. Controlling requires a multifaceted approach including whole genome sequencing support public health interventions at local and national levels. One of most widely used methods for is ARTIC protocol, tiling PCR followed by Oxford Nanopore (ONT) up 96 samples time. There need, however, flexible, platform agnostic, method that can provide multiple throughput options depending on...
The gut microbiota plays a crucial role in protecting against enteric infection. However, the underlying mechanisms are largely unknown owing to lack of suitable experimental models. Although most commensals anaerobic, intestinal epithelial cells require oxygen for survival. In addition, cell lines do not produce mucus, which provides habitat microbiota. Here, we have developed microaerobic, mucus-producing vertical diffusion chamber (VDC) model and determined influence Limosilactobacillus...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTReactions of homoleptic (alkyl isocyanide)molybdenum(II) cations [Mo(CNR)7]2+ with 2,2'-bipyridine and related ligands (N-N). Substitution chemistry dealkylation to form cyano-bridged complexes the type [{Mo(CNCMe3)4(N-N)}2(.mu.-CN)](PF6)3Andrew Bell, Stephen J. Lippard, Michael Roberts, Richard A. WaltonCite this: Organometallics 1983, 2, 11, 1562–1572Publication Date (Print):November 1, 1983Publication History Published online1 May 2002Published...
Two classes of cationic palladium(II) phosphine carboxylate complexes were isolated and characterized. Reactions trans-[(R3P)2Pd(O2CR')2] (1) with [Li(OEt2)2.5][B(C6F5)4] in MeCN led to abstraction formation trans-[(R3P)2Pd(O2CR')(MeCN)][B(C6F5)4] (2) good excellent yields. On the other hand, reactions 1 [Me2(H)NPh][B(C6F5)4] or p-toluenesulfonic acid (HOTs·H2O) CH2Cl2 furnished palladium cations [(R3P)2Pd(κ2-O,O-O2CR')]+ (3). The 2 3 Lewis bases found be different some cases. For example,...
Among a set of genes in pea (Pisum sativum L.) that were induced under drought-stress growth conditions, one encoded protein with significant similarity to regulator chlorophyll catabolism, SGR. This gene, SGRL, is distinct from SGR genomic location, carboxy-terminal motif, and expression through plant seed development. Divergence the two proteins associated loss intron/exon gene structure. Transient SGRL leaves Nicotiana benthamiana promoted degradation chlorophyll, manner was shown by...
Caesium trifluoroacetate has advantages over both sodium and caesium acetate for the efficient inversion of configuration alcohols by displacement triflates.
The silver(I) isocyanide complexes [Ag(CNR)4]ClO4(R = Me, cyclo-C6H11, or But) and [Ag(CNC6H11)2]ClO4 have been prepared, their i.r., Raman, 1H 13C n.m.r. spectra recorded, assignments proposed. Particular emphasis has placed on an assignment of the vibrational spectrum [Ag(CNMe)4]ClO4. Some reactions [Ag(CNBut)4]ClO4 with neutral Group 5 donor ligands examined. Complete displacement leads to [AgL4]ClO4[L PPh3, AsPh3, P(OMe)3]. mixed-ligand [Ag(CNR)(bipy)]ClO4(R C6H11 But; bipy...
Naturally occurring 2,7-anhydro-alpha-N-acetylneuraminic acid (2,7-anhydro-Neu5Ac) is a transglycosylation product of bacterial intramolecular trans-sialidases (IT-sialidases). A facile one-pot two-enzyme approach has been established for the synthesis 2,7-anhydro-sialic derivatives including those containing different sialic forms such as Neu5Ac and N-glycolylneuraminic (Neu5Gc). The based on use Ruminoccocus gnavus IT-sialidase release from glycoproteins, conversion free by aldolase. This...
Sialic acid (Sia) transporters are critical to the capacity of host-associated bacteria utilise Sia for growth and/or cell surface modification. While N-acetyl-neuraminic (Neu5Ac)-specific have been studied extensively, little is known on dedicated anhydro-Sia forms such as 2,7-anhydro-Neu5Ac (2,7-AN) or 2,3-dehydro-2-deoxy-Neu5Ac (Neu5Ac2en). Here, we used a Sia-transport-null strain
RgNanH is an intramolecular trans-sialidase expressed by the human gut symbiont Ruminococcus gnavus, to utilise intestinal sialylated mucin glycan epitopes. Its catalytic domain, belonging glycoside hydrolase GH33 family, cleaves off terminal sialic acid residues from mucins, releasing 2,7-anhydro-Neu5Ac which then used as metabolic substrate R. gnavus proliferate in mucosal environment. one of three trans-sialidases (IT-sialidases) characterised date, and first a commensal organism. Here,...
ABSTRACT Sialic acid (Sia) transporters are critical to the capacity of host-associated bacteria utilise sialic for growth and/or cell-surface modification. While N-acetyl-neuraminic (Neu5Ac)-specific have been studied extensively, little is known on dedicated anhydro-sialic forms such as 2,7-anhydro-Neu5Ac (2,7-AN) or 2,3-dehydro-2-deoxy-Neu5Ac (Neu5Ac2en). Here, we used a Sia-transport-null strain Escherichia coli investigate function members anhydro-Sia transporter families previously...