A5089083430- Pelvic floor disorders treatments
- Anorectal Disease Treatments and Outcomes
- Uterine Myomas and Treatments
- Endometriosis Research and Treatment
- Pelvic and Acetabular Injuries
- Urological Disorders and Treatments
- Tissue Engineering and Regenerative Medicine
- Hernia repair and management
- Gynecological conditions and treatments
- Urinary Tract Infections Management
- Pluripotent Stem Cells Research
- Pregnancy-related medical research
- Renal and related cancers
- Colorectal Cancer Surgical Treatments
- Bladder and Urothelial Cancer Treatments
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- Urinary Bladder and Prostate Research
- Biomedical Ethics and Regulation
- Reproductive System and Pregnancy
- Sexual function and dysfunction studies
- Nutritional Studies and Diet
- Ovarian cancer diagnosis and treatment
- TGF-β signaling in diseases
- Estrogen and related hormone effects
Stanford University
2015-2024
Menlo School
2021
Stanford Medicine
2006-2020
Palo Alto Institute
2018
National Institutes of Health
2005
Women's Health Initiative
2005
Medical College of Wisconsin
2005
To estimate the effects of estrogen plus progestin (E+P) therapy on menopausal symptoms, vaginal bleeding, gynecologic surgery rates, and treatment-related adverse in postmenopausal women.Randomized, double-blind, placebo-controlled trial 16,608 women, ages 50-79 (mean +/- standard deviation 63.3 7.1) years, with intact uterus, randomized to one tablet per day containing 0.625 mg conjugated equine estrogens 2.5 medroxyprogesterone acetate (n = 8,506) or placebo 8,102), followed for a mean...
Objective. The purpose of this study was to characterize color Doppler imaging features retained products conception (RPOC) with gray scale correlation. Methods. Clinically suspected cases RPOC between January 2005 and February 2008 were reviewed. Patient data relevant recorded. Results. A total 269 patients referred for sonographic evaluation identified. Thirty‐five had confirmed pathologic diagnoses, 28 whom RPOC. In those RPOC, 5 (18%) avascular (type 0); 6 (21%) minimal vascularity 1);...
Abstract Aims Weakening of pelvic supportive tissues is thought to be a contributing etiology in female floor disorders such as stress urinary incontinence and/or organ prolapse (SUI/POP). Since elastin modulates the mechanical properties tissues, we examined elastase activity vaginal tissue from women with dysfunction compared asymptomatic controls, by comparing overall activity, human neutrophil elastase, cathepsin K, and alpha‐1 antitrypsin (a serine protease inhibitor) mRNA protein...
Abdominal sacrocolpopexy is considered a standard of care operation for apical vaginal vault prolapse repair. Using outcomes at our center we evaluated whether the robotic approach to as cost-effective open approach.After obtaining institutional review board approval performed cost-minimization analysis in retrospective cohort patients who underwent institution between 2006 and 2010. Threshold values, that model variable values which most cost effective crosses over an alternative approach,...
Background: We developed an aligned bi-layered vascular graft derived from human induced pluripotent stem cells (iPSCs) that recapitulates the cellular composition, orientation, and anti-inflammatory function of blood vessels. Materials & methods: The luminal layer consisted longitudinal-aligned nanofibrillar collagen containing primary endothelial (ECs) or iPSC-derived ECs (iPSC-ECs). outer contained circumferentially oriented with smooth muscle (SMCs) SMCs(iPSC-SMCs). Results: On...
Abstract Background Growing laboratory and animal model evidence supports the potentially carcinogenic effects of some phthalates, chemicals used as plasticizers in a wide variety consumer products, including cosmetics, medications, vinyl flooring. However, prospective data on whether phthalates are associated with human breast cancer risk lacking. Methods We conducted nested case-control study within Women’s Health Initiative (WHI) cohort (n = 419 invasive case subjects 838 control...
The pelvic tissue of women with organ prolapse is stiffer than that controls but there are scant data on the collagen composition corresponds to these mechanical properties. We evaluated human vaginal wall stiffness using novel scanning haptic microscope and correlated measurements expression in without prolapse. In this simultaneous biomechanical biochemical assessment we usefulness measurement technology for floor disorder research confirmed an association between properties composition.
Abstract Smooth muscle cells (SMCs) and endothelial (ECs) are typically derived separately, with low efficiencies, from human pluripotent stem (hPSCs). The concurrent generation of these cell types might lead to potential applications in regenerative medicine model, elucidate, eventually treat vascular diseases. Here we report a robust two-step protocol that can be used simultaneously generate large numbers functional SMCs ECs common proliferative progenitor population via two-dimensional...
There is great interest in using stem cells (SC) to regenerate a deficient urethral sphincter patients with urinary incontinence. The smooth muscle component of the significant contributor function. However, current translational efforts for restoration focus only on skeletal regeneration because they rely adult mesenchymal SC as cell source. These do not yield sufficient (SMCs) transplantation. We may be able overcome this limitation by pluripotent (PSC) derive SMCs. Hence, we sought...
Recent studies showing a correlation between descent of the anterior and apical vaginal compartments suggest that cystoceles may recur if associated prolapse is not corrected. However, to date anatomical relationship with respect cystocele has been incompletely reported. We present predictive value for clinically significant prolapse.We retrospectively reviewed records all new patient visits urogynecology clinic in 30-month period. Women point Ba -1 or greater (stage 2 above) were included...
Abstract Aims To report the findings of a multidisciplinary group scientists focusing on issues in basic science and translational research related to female pelvic floor disorders, produce recommendations for agenda investigators studying disorders. Methods A National Institutes Health (NIH)‐sponsored meeting was held November 14–15, 2002, bringing together diverse fields including obstetrics, gynecology, urogynecology, urology, gastroenterology, biomechanical engineering, neuroscience,...
BACKGROUND: The pathophysiology of pelvic floor dysfunction resulting in stress urinary incontinence (SUI) women is complex. Evidence suggests that there also a genetic predisposition towards SUI. We sought to identify differentially expressed genes involved extracellular matrix (ECM) metabolism vaginal tissues from with SUI the secretory phase menses compared asymptomatic women. METHODS: Tissue samples were taken periurethral wall five pairs premenopausal, age-matched and continent...
Automated cell segmentation and tracking is essential for dynamic studies of cellular morphology, movement, interactions as well other behaviors. However, accurate, automated, easy-to-use remains a challenge, especially in cases high densities, where discrete boundaries are not easily discernable. Here, we present fully automated algorithm that iteratively segments cells based on the observed distribution optical volumes measured by quantitative phase microscopy. By fitting these...
We compare the cost-effectiveness of 3 strategies for use a mid urethral sling to prevent occult stress urinary incontinence in patients undergoing abdominal sacrocolpopexy.Using decision analysis modeling we compared during 1-year postoperative period treatment approaches including 1) sacrocolpopexy alone with deferred option sling, 2) universal concomitant and 3) preoperative urodynamic study selective sling. Using published data modeled probabilities after or without predictive value...
Abstract It is unclear which of four popular contemporary diet patterns best for weight maintenance among postmenopausal women. Four dietary were characterised women aged 49–81 years (mean 63·6 ( sd 7·4) years) from the Women’s Health Initiative Observational Study: (1) a low-fat diet; (2) reduced-carbohydrate (3) Mediterranean-style (Med) and (4) consistent with US Department Agriculture’s Dietary Guidelines Americans (DGA). Discrete-time hazards models used to compare risk gain (≥10 %)...
BACKGROUND: To evaluate differential expression of transforming growth factor (TGF-β1), latent factor-binding proteins (LTBP-1, LTBP-2) and elastin microfibril components (fibrillin-1 fibrillin-2) in vaginal tissue from women with stress urinary incontinence (SUI). METHODS: In this case–control study, both phases the menstrual cycle was obtained. Messenger RNA (mRNA) expressions LTBP-1, LTBP-2, fibrillin-1, fibrillin-2 TGF-β1 were determined by relative real-time quantification PCR. Tissue...
Abstract We aimed to derive induced pluripotent stem cell (iPSC) lines from vaginal fibroblasts older women with pelvic organ prolapse. examined the effect of donor age on iPSCs and cells redifferentiated these iPSCs. Vaginal were isolated younger subjects for reprogramming. generated simultaneously using an excisable polycistronic lentiviral vector expressing Oct4, Klf4, Sox2, cMyc. The markers confirmed by immunocytochemistry quantitative reverse transcription-polymerase chain reaction...