A5059826429- Herpesvirus Infections and Treatments
- Influenza Virus Research Studies
- Vector-borne infectious diseases
- Virology and Viral Diseases
- Animal Disease Management and Epidemiology
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Vector-Borne Animal Diseases
- Reproductive tract infections research
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Microbial infections and disease research
- Cytomegalovirus and herpesvirus research
- Insect symbiosis and bacterial influences
- Rabies epidemiology and control
- Virus-based gene therapy research
- Respiratory viral infections research
- Bird parasitology and diseases
- Yersinia bacterium, plague, ectoparasites research
- Bacteriophages and microbial interactions
- Syphilis Diagnosis and Treatment
- Leptospirosis research and findings
- Urinary Tract Infections Management
- T-cell and Retrovirus Studies
- Trypanosoma species research and implications
Tottori University
2016-2025
Yamaguchi University
2010-2016
Gifu University
2003-2012
Hiroshima University
2011
National Institute of Health Sciences
2008
Graduate School USA
2006
Kure Medical Center
2004
Army Medical University
2002
Imperial Household Agency
1997
Kyoto City Hospital
1995
H5N1 highly pathogenic avian influenza virus (HPAIV) was reintroduced and caused outbreaks in chickens the 2010-2011 winter season Japan, which had been free from (HPAI) since 2007 when HPAI occurred were controlled. On 14 October 2010 at Lake Ohnuma, Wakkanai, northernmost part of Hokkaido, HPAIVs isolated faecal samples ducks flying their nesting lakes Siberia. Since then, have 63 wild birds 17 prefectures 24 chicken farms nine by end March 2011. Each these isolates genetically closely...
ABSTRACT Q fever is a zoonotic disease of worldwide significance caused by the obligate intracellular bacterium Coxiella burnetii . Humans with may experience an acute flu-like illness and pneumonia and/or chronic hepatitis or endocarditis. Various markers demonstrate significant phylogenetic separation between clustering among isolates from human disease. The clinical pathological responses to infection phase I C. following four genomic groups were evaluated in immunocompetent...
The prevalence of Coxiella burnetii infection in 207 cattle with reproductive disorders was studied by using an indirect immunofluorescence (IF) test, nested polymerase chain reaction (PCR) and isolation. IF antibodies to phase I II antigens C. were found 122 (58.9%) 125 (60.4%) the sera, respectively, PCR-positives 8 (3.9%) sera 51 (24.6%) milk samples. In addition, isolated from samples inoculating laboratory mice. results indicate that test plus PCR are useful diagnosis bovine...
Abstract Coxiella burnetii was isolated from raw milk (36/214, 16.8%) and uterus swab samples (13/61, 21.3%) originating dairy cattle with reproductive disorders, aborted bovine fetus (2/4, 50%), mammary gland (4/50, 8%) healthy cattle, tick (4/15, 26.7%) 2 pastures. Fifty‐nine strains had various degrees of pathogenicity, high (8; 13.6%), moderate (28; 47.5%) low (23; 39%), for guinea pigs. The results isolation suggested a prevalence infection in problems Japan. Twelve (7, 3 ticks humans,...
Newcastle disease virus (NDV) is a causative agent of (ND), major infectious poultry associated with significant economic losses. Vaccination usually effective at preventing the disease. However, in Ethiopia, ND commonly detected both unvaccinated and vaccinated chickens. In this study, we aimed to evaluate pathogenicity NDV isolated from chickens, as well compare antigenicity isolates vaccine strains genotyping by using F-gene sequence. The partial F gene sequences all mean death times...
ABSTRACT A nested PCR method was developed for the detection of Coxiella burnetii in human serum samples. Two pairs oligonucleotide primers were designed to amplify a 438-bp fragment com1 gene encoding 27-kDa outer membrane protein C. . The amplified predicted fragments 21 various strains but did not react with DNA samples from other microorganisms. amplification products could be digested restriction enzymes Ssp I and Sal I. utility evaluated by testing 135 (87%) 155 indirect...
Chlamydophila felis (C. felis), feline herpesvirus-1 (FHV-1) and calicivirus (FCV) were detected in 39 (59.1%), 11 (16.7%) 14 (21.2%) cats respectively, from 66 domestic presented with conjunctivitis upper respiratory tract disease (URTD) 9 prefectures of Japan. Dual multiple infections found 7 (10.6%) both C. FHV-1, 10 (15.2%) FCV, 1 (1.5%) cat all three agents. was isolated (28.2%) PCR positive cats. Antigenic difference a 96 kDa protein our isolates Fe/145 strain USA. In conclusion, is...
The prevalence of Q fever pneumonia among children with atypical from whom only an acute-phase serum sample was available traced by using indirect immunofluorescence (IF) test, nested PCR, and isolation. Twenty (34.5%) 58 sera were found to have both polyvalent immunoglobulin M antibodies the phase II antigen Coxiella burnetii IF test. present in 23 (39.7%) patients as determined PCR isolation 20 sensitivities for 100%, that test 87%. Our results indicate faster more sensitive than diagnosis...
One hundred and thirty-four (26%) of 511 sera from 11 wild animal species in eight prefectures Japan had antibody titers to Coxiella burnetii by the enzyme-linked immunosorbent assay. High prevalences were observed Japanese black bears (Ursus thibetanus) (78%), Hokkaido deer (Cervus nippon yesoensis) (69%), hares (Lepus brachyurus) (63%), centralis) (56%), some extent monkeys (Macaca fuscata) (28%). A low prevalence (13%) was nutrias (Myocastor coypus). serows (Capricornis crispus), rats...
To elucidate the evolutionary pathway, we sequenced entire genomes of 89 H5N6 highly pathogenic avian influenza viruses (HPAIVs) isolated in Japan during winter 2016–2017 and 117 AIV/HPAIVs Russia. Phylogenetic analysis showed that at least 5 distinct genotypes HPAIVs affected poultry wild birds period. Japanese isolates shared a common genetic ancestor 6 8 genomic segments, PA NS genes demonstrated 4 2 origins, respectively. Six gene segments originated from putative ancestral clade 2.3.4.4...
Summary Virologic and pathologic investigations were done on prednisolone-treated bitches with a history of canine herpesvirus ( chv ) infection. Reactivation was demonstrated in 5 Beagle after daily administration 600 mg prednisolone for days. The reactivation confirmed 4 bitches. Canine recovered from nasal, oral, vaginal, ocular secretions the 5th to 21st days initiation treatment prednisolone, also nasal mucosa tonsil tissues. Results indicated that latent infections develop virus may be...
SUMMARY Fetal infection was established in 28 of 33 pups obtained from 7 pregnant dogs (47th to 53rd day gestation) inoculated iv with canine herpesvirus ( chv ). The pathogenesis transplacental investigated by using pathologic examination, immunofluorescence microscopy, and viral assay. Infection the virus recognized 13 18 neonatal 4 bitches spontaneous delivery at full term. Eleven died within a week birth all had characteristic lesions infection. Thirteen live-born 2 stillborn derived 3...
Poultry red mite (PRM, Dermanyssus gallinae) is a blood-sucking ectoparasite as well possible vector of several avian pathogens. In this study, to define the role PRM in prevalence infectious agents, we used polymerase chain reaction (PCR) check for presence seven pathogens: Avipox virus (APV), Fowl Adenovirus (FAdV), Marek's disease (MDV), Erysipelothrix rhusiopathiae (ER), Salmonella enterica (SE), Mycoplasma synoviae (MS) and gallisepticum (MG). A total 159 samples collected between 2004...
In the winter of 2021-2022, multiple subtypes (H5N8 and H5N1) high pathogenicity avian influenza viruses (HPAIVs) were confirmed to be circulating simultaneously in Japan. Here, we phylogenetically antigenically analyzed HPAIVs that isolated from infected wild birds, an epidemiological investigation affected poultry farms, our own active surveillance study. H5 subtype hemagglutinin (HA) genes 32 representative HPAIV isolates classified into clade 2.3.4.4b lineage subsequently divided three...
ABSTRACT Q fever, a worldwide zoonosis caused by Coxiella burnetii , has many manifestations in humans. Endocarditis is the most serious complication of fever. Animal models are limited to acute pulmonary or hepatic disease and reproductive disorders. An appropriate experimental animal model for fever endocarditis does not yet exist. In this study, severe combined immunodeficient (SCID) mice infected with C. showed persistent clinical symptoms died, whereas immunocompetent similarly became...
Some strains of highly virulent infectious bursal disease virus (HV-IBDV) were adapted through serial passage in embryonated eggs. The egg-adapted HV-IBDV was successfully to grow chicken embryo fibroblast (CEF) cell cultures showing a cytopathic effect by preparing the CEF cells from virus-infected embryos. egg- and culture-adapted significantly reduced their pathogenicity to, did not kill any, young chickens experimental infection. lesions strain-infected similar those observed classical...
We evaluated the prevalence of Coxiella burnetii antibodies in 626 human serum samples (275 from veterinarians, 107 meat-processing workers, 184 respiratory-disorder patients, and 60 healthy humans) by indirect immunofluorescence test. Of examined, 54 (8.6%) 103 (16.5%) reacted positively to phase I II antigens, respectively, C. burnetii. The rates differed for humans patients. Antibody was high living close contact with animals (e.g., veterinarians workers).
To study genetic diversity and occurrence of Chlamydophila psittaci, a total 1,147 samples from 11 avian orders including 53 genera 113 species feral captive birds were examined using ompA gene based nested PCR. Three types chlamydiae: C. psittaci (94.12%), abortus (4.41%) unknown sp. (1.47%) identified among 68 (5.93%) positive (Psittaciformes-59, Ciconiiformes-8 Passeriformes-1). Based on nucleotide sequence variations in the VD2 region gene, all 64 detected strains grouped into 4...
Abstract The gene (com1) encoding a 27‐kDa outer membrane protein in 21 strains of Coxiella burnetii from variety clinical and geographical sources was sequenced for strain differentiation. com1 highly conserved among all the tested but there were several differences nucleotide deduced amino acid sequences. Based on gene‐specific nucleotides acids, divided into four groups. Group 1 contained 14 originating ticks, cattle human cases acute Q fever. Groups 2 3 included strains, respectively,...