A5101468592- Alzheimer's disease research and treatments
- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Epilepsy research and treatment
- Neuroscience and Neuropharmacology Research
- Chemical Synthesis and Analysis
- Regulation of Appetite and Obesity
- Hepatitis B Virus Studies
- Lipid Membrane Structure and Behavior
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Pharmacological Effects and Toxicity Studies
- Protein Structure and Dynamics
- T-cell and Retrovirus Studies
- SARS-CoV-2 and COVID-19 Research
- Hepatitis C virus research
- Immune Cell Function and Interaction
- Influenza Virus Research Studies
- COVID-19 Clinical Research Studies
- Biochemical Analysis and Sensing Techniques
- Ion Transport and Channel Regulation
- Animal Disease Management and Epidemiology
- Hormonal Regulation and Hypertension
- Enzyme Production and Characterization
- Adipose Tissue and Metabolism
Tokyo Metropolitan Institute of Medical Science
1986-2024
Fujita Health University
2004-2024
Kao Corporation (Japan)
2006-2023
International University of Health and Welfare
2023
Nagasaki University
1999-2022
Osaka University
2010-2022
Nagasaki University Hospital
2022
Hokkaido University
2011-2021
Ritsumeikan University
2009-2020
Hokuriku University
2014-2020
A fundamental question about the early pathogenesis of Alzheimer's disease (AD) concerns how toxic aggregates amyloid beta protein (Abeta) are formed from its nontoxic soluble form. We hypothesized previously that GM1 ganglioside-bound Abeta (GAbeta) is involved in process. now examined this possibility using a novel monoclonal antibody raised against GAbeta purified an AD brain. Here, we report has conformation distinct and initiates aggregation by acting as seed. Furthermore, generation...
Aldosterone mediates actions of the renin-angiotensin-aldosterone system inducing hypertension, oxidative stress, and vascular inflammation. Recently, we showed that angiotensin II–induced hypertension damage are mediated at least in part by macrophages T-helper effector lymphocytes. Adoptive transfer suppressor T-regulatory lymphocytes (Tregs) prevented II action. We hypothesized Treg adoptive would blunt aldosterone-induced damage. Thirteen to 15-week–old male C57BL/6 mice were injected...
Epidemiologic studies indicate that ingestion of vegetables and fruit inhibits the development cardiovascular disease. Chlorogenic acids are abundant phenolic compounds contained in fruits, but impact dietary chlorogenic on vascular function hypertension is not known. We therefore examined effects 5-caffeoylquinic acid (CQA), a representative acid, blood pressure age-matched normotensive Wistar-Kyoto rats spontaneously hypertensive rats.A single CQA (30-600 mg/kg) reduced rats, an effect was...
The innate immune system is essential for controlling viral infection. Hepatitis B virus (HBV) persistently infects human hepatocytes and causes hepatocellular carcinoma. However, the response to HBV infection in vivo remains unclear. Using a tree shrew animal model, we showed that induced hepatic interferon (IFN)-γ expression during early Our vitro study demonstrated NK cells produced IFN-γ only presence of F4/80+ cells. Moreover, extracellular vesicles released from HBV-infected contained...
—Angiotensin type 2 receptor–mediated effects of angiotensin II appear to counteract many the mediated via 1 receptor. Compound 21 (C21), a selective receptor agonist, has demonstrated beneficial on cardiac function after myocardial infarction in rodents. We hypothesized that C21 alone or combination with an antagonist would blunt development hypertension and vascular damage stroke-prone spontaneously hypertensive rats. Six-week–old rats received (1 mg/kg per day), losartan (10 plus...
The chemical synthesis of complex glycoproteins is an ongoing challenge in protein chemistry. We have examined the a single glycoform monocyte chemotactic protein-3 (MCP-3), CC-chemokine that consists 76 amino acids and one N-glycosylation site. A three-segment native ligation strategy was employed using unprotected peptides glycopeptide. Importantly, required development methods for generation sialylglycopeptide-alphathioesters. For sialylglycopeptide-alphathioester segment, we successfully...
The membrane pore proteins, aquaporins (AQPs), facilitate the osmotically driven passage of water and, in some instances, small solutes. Under hyperosmotic conditions, expression AQPs changes, and studies have shown that AQP1 AQP5 is regulated by MAPKs. However, mechanisms regulating AQP4 AQP9 induced stress are poorly understood. In this study, we observed mannitol increased cultured rat astrocytes, intraperitoneal infusion brain cortex. addition, a p38 MAPK inhibitor, but not ERK JNK...
Abstract We describe herein the preparation of 24 pure asparagine‐linked oligosaccharides (Asn‐oligosaccharides) from biantennary complex‐type sialylundecasaccharide [(NeuAc‐α‐2,6‐Gal‐β‐1,4‐GlcNAc‐β‐1,2‐Man‐α‐1,6/1,3‐) 2 ‐Man‐β‐1,4‐GlcNAc‐β‐1,4‐GlcNAc‐β‐1‐asparagine, ] obtained egg yolk. Our synthetic strategy aimed at adapting branch specific exo‐glycosidases digestion (β‐ D ‐galactosidase, N‐acetyl‐β‐ ‐glucosaminidase and α‐ ‐mannosidase) individual asialo‐branch after...
Endothelium-derived nitric oxide (EDNO) plays an important role in the regulation of angiogenesis, whereas hypercholesterolemia (HC) impairs EDNO release. We examined hypothesis that HC may inhibit ischemia-induced angiogenesis by inhibition a rat model unilateral hindlimb ischemia and oral L-arginine supplementation, substrate for NO synthase, prevent HC-related impairment angiogenesis.Male Sprague-Dawley rats were fed (A) standard diet (control), (B) 2% high-cholesterol (HC group), or (C)...
H5N1 highly pathogenic avian influenza virus (HPAIV) was reintroduced and caused outbreaks in chickens the 2010-2011 winter season Japan, which had been free from (HPAI) since 2007 when HPAI occurred were controlled. On 14 October 2010 at Lake Ohnuma, Wakkanai, northernmost part of Hokkaido, HPAIVs isolated faecal samples ducks flying their nesting lakes Siberia. Since then, have 63 wild birds 17 prefectures 24 chicken farms nine by end March 2011. Each these isolates genetically closely...
The aim of this study was to investigate the in vivo and vitro effects exendin-4, a potent glucagon-like peptide 1 agonist, on protection pancreatic beta-cells against their cell death. In experiments, we used beta-cell-specific calmodulin-overexpressing mice where massive apoptosis takes place beta-cells, examined chronic treatment with exendin-4. Chronic s.c. administration exendin-4 reduced hyperglycemia. caused significant increases insulin contents pancreas islets, retained...
Increased oxidative damage is a prominent and early feature in Alzheimer disease. We previously crossed disease transgenic (APPsw) model mice with α-tocopherol transfer protein knock-out (Ttpa−/−) which lipid peroxidation the brain was significantly increased. The resulting double-mutant (Ttpa−/−APPsw) showed increased amyloid β (Aβ) deposits brain, ameliorated supplementation. To investigate mechanism of Aβ accumulation, we here studied generation, degradation, aggregation, efflux mice....
In mammals, leptin released from the white adipose tissue acts on central nervous system to control feeding behavior, cardiovascular function, and energy metabolism. Central activates sympathetic nerves that innervate kidney, tissue, some abdominal organs in rats. AMP-activated protein kinase (AMPK) is essential intracellular signaling pathway involving activation of receptors (ObRb). We investigated potential AMPKα2 effects using vivo siRNA injection knockdown rats, produce reduced...
One of the major neuropathological hallmarks Alzheimer's disease (AD) is deposition amyloid β-protein (Aβ) in brain. Aβ accumulation seems to arise from an imbalance between production and clearance. Neprilysin (NEP) insulin-degrading enzyme (IDE) are important Aβ-degrading enzymes brain, deficits their expression may promote patients with sporadic late-onset AD. Statins, which used clinically for reducing cholesterol levels, can exert beneficial effects on Therefore, we examined whether...
Leptin action in the brain has emerged as an important regulator of liver function independently from its effects on food intake and body weight. The autonomic nervous system plays a key role regulation physiological processes by leptin. Here, we used direct recording nerve activity sympathetic or vagal nerves subserving to investigate how leptin controls hepatic activity. Intracerebroventricular (ICV) administration activated traffic rats mice dose- receptor-dependent manners....
Abstract Interleukin (IL)‐1β is known to play a role in the formation of brain edema after various types injury. Aquaporin (AQP)4 also reported be involved progression edema. We tested hypothesis that AQP4 induced response IL‐1β. found expression mRNA and protein was significantly up‐regulated by IL‐1β cultured rat astrocytes, intracerebroventricular administration increased brain. The effects on induction were concentration time dependent. mediated through receptors because they abolished...
Abstract Exosomes are extracellularly released small vesicles that derived from multivesicular bodies formed via the endocytic pathway. We treated pheochromocytoma PC12 cells with chloroquine, an acidotropic agent, which potently perturbs membrane trafficking endosomes to lysosomes. Chloroquine treatment increased level of GM1 ganglioside in cell media only when were exposed KCl for depolarization, is known enhance exosome release neurons. In sucrose‐density‐gradient fractionation media, was...
Antiviral agents including entecavir (ETV) suppress the replication of hepatitis B virus (HBV) genome in human hepatocytes, but they do not reduce abundance viral proteins. The present study focused on effectively reducing protein levels.We designed siRNAs (HBV-siRNA) that target consensus sequences HBV genomes. To prevent emergence escaped mutant virus, we mixed three HBV-siRNAs (HBV-siRNAmix); mixture was encapsulated a novel pH-sensitive multifunctional envelope-type nanodevice (MEND),...
An extracellular vesicle (EV) is a nanovesicle that shuttles proteins, nucleic acids, and lipids, thereby influencing cell behavior. A recent crop of reports have shown EVs are involved in infectious biology, host immunity playing role the viral life cycle. In present work, we investigated EV-mediated transmission hepatitis B virus (HBV) infection.We HBV infection by using culture system uses primary human hepatocytes derived from humanized chimeric mice (PXB-cells). Purified were isolated...