A5001314715- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Lipid Membrane Structure and Behavior
- Influenza Virus Research Studies
- Chemical Synthesis and Analysis
- Alzheimer's disease research and treatments
- Reproductive biology and impacts on aquatic species
- Carbohydrate Chemistry and Synthesis
- Supramolecular Self-Assembly in Materials
- Aquaculture Nutrition and Growth
- Marine Sponges and Natural Products
- Microbial Natural Products and Biosynthesis
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Crystallization and Solubility Studies
- Galectins and Cancer Biology
- Seaweed-derived Bioactive Compounds
- Animal Genetics and Reproduction
- X-ray Diffraction in Crystallography
- Click Chemistry and Applications
- Microfluidic and Bio-sensing Technologies
- Machine Learning in Bioinformatics
- Advanced Proteomics Techniques and Applications
Keio University
2015-2024
Okayama University
1997-2017
Ehime University
2011
Osaka Prefecture University
2008
Tokyo Institute of Technology
1999-2006
Otsuka (Japan)
2006
The University of Tokyo
2005
Diamond Materials (United States)
2005
Shibaura Institute of Technology
2005
Japan Fisheries Research and Education Agency
2003-2004
Influenza is an infectious disease caused by the influenza virus, and each year many people suffer from this disease. Hemagglutinin (HA) in membrane of type A viruses recognizes sialylglycoconjugate receptors on host cell surface at initial step infection process; consequently, HA inhibitors are considered potential candidates for antiviral drugs. We identified peptides that bind to receptor-binding sites through a multiple serial selection phage-displayed random peptide libraries. Using H1...
We designed glycopolymers carrying sialyl oligosaccharides by "post-click" chemistry and evaluated the interaction with influenza virus. The glycopolymer structures were synthesized in a well-controlled manner reversible addition–fragmentation chain transfer polymerization Huisgen reaction. Acrylamide-type monomers copolymerized to give hydrophilicity polymer backbones, enabled successful introduction of into backbones. different sugar densities lengths for hemagglutinin on virus surface....
Significance A large number of people suffer from influenza every year. Early detection is critical for preventing severe in high-risk groups. There usually not enough time to produce vaccines against pandemic viruses before they spread other countries. Anti-influenza therapy using neuraminidase inhibitors effective within 30 h the onset symptoms. Here we show that several dozen plaque-forming units virus (IFV) are detectable by a boron-doped diamond electrode terminated with sialic...
The precise design of synthetic polymer ligands using controlled polymerization techniques provides an advantage for the field nanoscience. We report topological glyco-ligands based on polymers targeting hemagglutinin (HA, lectin influenza virus). To achieve arrangement glycounits toward sugar-binding pockets HA, triarm star glycopolymers were synthesized. interaction with HA was found to depend length arms and maximized when hydrodynamic diameter glycopolymer comparable distance between HA....
The development of a simple detection method with high sensitivity is essential for the diagnosis and surveillance infectious diseases. Previously, we constructed sensitive biosensor pathological human influenza viruses using boron-doped diamond electrode terminated sialyloligosaccharide receptor-mimic peptide that could bind to hemagglutinins involved in viral infection. Circulation induced by avian virus humans has become major public health concern, methods are urgently needed. Here,...
A combinatorial phage display method was applied to films composed of a stereoregular polymer methacrylates. The clones with selective affinity for isotactic (it) poly(methyl methacrylate) (PMMA) were isolated. Greater amounts the bound it-PMMA, compared other PMMAs. expressing ELWRPTR most strongly polymer, and selectivity also best. peptide motif essential specific interaction revealed.
Influenza virus hemagglutinin recognizes sialyloligosaccharides of glycoproteins and glycolipids as cell surface receptors in the initial stage infection process. We demonstrate that pentadecapeptides bind to a sialylgalactose structure (Neu5Ac−Gal) inhibited cells by influenza virus. The were identified through affinity selection from phage-displayed random peptide library using monolayer ganglioside Neu5Acα2−3Galβ1−4Glcβ1−1′Cer (GM3). peptides found have for GM3, alanine scanning showed...
A series of carbosilane dendrimers uniformly functionalized with hemagglutinin (HA) binding peptide (sialic acid-mimic peptide, Ala-Arg-Leu-Pro-Arg) was systematically synthesized, and their anti-influenza virus activity evaluated. The carbosilane-based dendrimers, unlike sialylated cannot be digested by neuraminidases. exhibited intriguing biological activities depending on the form core frame, a dumbbell-type dendrimer showing particularly strong inhibitory against two human influenza...
Biological membranes are functionalized by membrane-associated protein machinery. Membrane-associated transport processes, such as endocytosis, represent a fundamental and universal function mediated membrane-deforming machines, which small biomolecules even micrometer-size substances can be transported via encapsulation into membrane vesicles. Although synthetic molecules that induce dynamic deformation have been reported, molecular approach enabling in is coupled with substance binding...
Type 2 diabetes mellitus is thought to be a significant risk factor for Alzheimer's disease. Insulin resistance also affects the central nervous system by regulating key processes, such as neuronal survival and longevity, learning memory. However, mechanisms underlying these effects remain uncertain. To investigate whether insulin associated with assembly of amyloid β-protein (Aβ) at cell surface neurons, we inhibited insulin-signalling pathways primary neurons. The treatments receptor...
Amyloid deposition, a crucial event of Alzheimer's disease (AD), emerges in distinct brain regions. A key question is what triggers the assembly monomeric amyloid ß-protein (Aß) into fibrils On basis our previous findings that gangliosides facilitate initiation Aß at presynaptic neuritic terminals, we investigated how lipids, including gangliosides, cholesterol and sphingomyelin, extracted from synaptic plasma membranes (SPMs) isolated autopsy brains were involved assembly. We focused on two...
The deposition of amyloid β-protein (Aβ) is a pathological hallmark Alzheimer's disease (AD). We previously found that the ganglioside-enriched microdomains (ganglioside clusters) in presynaptic neuronal membranes play key role initiation Aβ assembly process. However, not all ganglioside clusters accelerate assembly. In present study, we directly observed spherical an atomic force microscopic study on morphology reconstituted lipid bilayer composed lipids were extracted from...
Ganglioside-enriched microdomains in the presynaptic neuronal membrane play a key role initiation of amyloid ß-protein (Aß) assembly related to Alzheimer's disease. We previously isolated lipids from detergent-resistant microdomain fraction synaptosomes prepared aged mouse brain and found that spherical Aß assemblies were formed on Aß-sensitive ganglioside nanoclusters (ASIGN) reconstituted lipid bilayers synaptosomal fraction. In present study, we investigated oligosaccharides fibril...
An antimalarial lipopeptide, ikoamide, was isolated from an Okeania sp. marine cyanobacterium. Its gross structure established by spectroscopic analyses, and the absolute configuration clarified based on a combination of chiral-phase HPLC derivatization reactions. Ikoamide showed strong activity with IC50 value 0.14 μM without cytotoxicity against human cancer cell lines at 10 μM.
Ganglioside Gal beta1 --> 3GalNAc 4(NeuAc alpha2 3) 4Glc -->1'Cer (GM1)-binding peptides were obtained from a phage-displayed pentadecapeptide library by an affinity selection. The selection processes in situ-monitored quartz-crystal microbalance method, on which ganglioside GM1 monolayer was transferred. After five rounds of biopanning, the DNA sequencing 18 selected phages showed that only three individual clones selected. peptide sequences random region found to be DFRRLPGAFWQLRQP,...
Motobamide (1), a new cyclic peptide containing C-prenylated cyclotryptophan residue, was isolated from marine Leptolyngbya sp. cyanobacterium. Its planar structure established by spectroscopic and MS/MS analyses. The absolute configuration elucidated based on combination of chemical degradations, chiral-phase HPLC analyses, computational chemistry. (1) moderately inhibited the growth bloodstream forms Trypanosoma brucei rhodesiense (IC50 2.3 μM). However, it exhibited weaker cytotoxicity...
Glycoconjugates play various roles in biological processes. In particular, oligosaccharides on the surface of animal cells are involved virus infection and cell-cell communication. Inhibitors carbohydrate-protein interactions potential antiviral drugs. Several anti-influenza drugs such as oseltamivir zanamivir derivatives sialic acid, which inhibits neuraminidase. However, it is very difficult to prepare a diverse range sugar by chemical synthesis or isolation natural products. addition,...
Synthetic glyco-ligands are promising candidates for effective nanomedicines against pathogens. Glycopolymers bearing sialyl-oligosaccharides interact with hemagglutinin present on the surface of influenza viruses. In designing new glycopolymers that further enhance interaction viruses, both static and dynamic properties should be considered. this report, we evaluated correlation between their virus. pendant sialyllactoses various linker structures were synthesized, molecular mobility was...
Abstract An optically-active redox amino acid, l-ferrocenylalanine was synthesized. The acid incorporated into polypeptides in the form of a single unit or l-ferrocenylalanyl-l-ferrocenylalanine unit. Those showed reversible property on cyclic voltammetry.
We obtained a novel carbohydrate-binding peptide having helix−loop−helix scaffold from random library. The library randomized at five amino acid residues was displayed on the major coat protein of filamentous phage. Affinity selection with ganglioside, Galβ1−3GalNAcβ1−4(Neu5Acα2−3)Galβ1−4Glcβ1−1′Cer (GM1), gave positive phage clones. Surface plasmon resonance spectroscopy showed that corresponding 35-mer synthetic had high affinity for GM1 dissociation constant 0.24 µM. This preferentially...