A5068639454- Lipid Membrane Structure and Behavior
- Advanced biosensing and bioanalysis techniques
- Innovative Microfluidic and Catalytic Techniques Innovation
- Alzheimer's disease research and treatments
- Supramolecular Self-Assembly in Materials
- Nanopore and Nanochannel Transport Studies
- Microfluidic and Capillary Electrophoresis Applications
- Membrane Separation Technologies
- RNA Interference and Gene Delivery
- Bacteriophages and microbial interactions
- Electrowetting and Microfluidic Technologies
- X-ray Diffraction in Crystallography
- Microbial Community Ecology and Physiology
- 3D Printing in Biomedical Research
- Crystallization and Solubility Studies
- DNA and Biological Computing
- Surfactants and Colloidal Systems
- Molecular Communication and Nanonetworks
- Electrostatics and Colloid Interactions
- Tea Polyphenols and Effects
- Microfluidic and Bio-sensing Technologies
- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- Glycosylation and Glycoproteins Research
- Nanoparticle-Based Drug Delivery
National Institute of Advanced Industrial Science and Technology
2018-2024
Tokyo Institute of Technology
2013-2024
Japan Advanced Institute of Science and Technology
2009-2014
Significance Although liposomes and lipid droplets have been used for numerous applications, the fragility of membrane causes an unintentional collapse, which is problematic advanced applications. To solve this problem, we constructed artificial cytoskeleton with DNA nanotechnology (a cytoskeleton). The a network formed underneath positively charged lipids through electrostatic interactions without need special handling. significantly improves mechanical stability and, therefore, confers...
It is important that we understand the physical, chemical, and biological mechanisms govern interaction between nanoparticles (NPs) heterogeneous cellular surfaces because of possible cytotoxicity engineered nanomaterials. In this study, investigated lateral localization nano/microparticles within a biomimetic membrane interface using cell-sized two-phase liposomes. We found heterogeneity in mediates partitioning size-dependent manner: small particles with diameter ≤200 nm were localized an...
A multiblock amphiphilic molecule 1, with a tetrameric alternating sequence of hydrophilic and hydrophobic units, adopts folded structure in liposomal membrane like multipass transmembrane protein, is able to transport alkali metal cations through the membrane. Hill's analysis conductance measurements, analyzed by Hille equation, revealed that assembly 1 forms 0.53 nm channel allowing for permeation cations. Since neither 3, bearing flexible units forming no stacked structures membrane, nor...
Abstract We report a centrifugal microfluidic method, droplet‐shooting and size‐filtration (DSSF), for the production of cell‐sized liposomes with controlled lipid compositions. This involves generation large small droplets from tip glass capillary selective transfer through an oil‐water interface, thus resulting in liposomes. demonstrate control microdomain formation as well asymmetric bilayer uniform size by composition. The DSSF method simple microfluidics is easy to use. In addition,...
Abstract Self‐assembly of designed nanostructures on a lipid membrane offers powerful approach for enhancing the functionality its surface. Here, environment‐dependent assembly blunt‐ended DNA origami structures phase‐separated lipid‐bilayer consisting liquid‐disordered (Ld) and solid‐ordered (So) phases is reported. Fluorescence microscopy atomic force (AFM) imaging reveal that preferentially bind to So phase. At higher concentrations, origamis form 2D lattices Ld phase through...
Biological membranes are functionalized by membrane-associated protein machinery. Membrane-associated transport processes, such as endocytosis, represent a fundamental and universal function mediated membrane-deforming machines, which small biomolecules even micrometer-size substances can be transported via encapsulation into membrane vesicles. Although synthetic molecules that induce dynamic deformation have been reported, molecular approach enabling in is coupled with substance binding...
We used a cell-sized model system, giant liposomes, to investigate the interaction between lipid membranes and surfactants, membrane transformation during solubilization process was captured in real time. found that there are four distinct dynamics surfactant-induced deformation: an episodic increase area prior pore-forming associated shrinkage (Dynamics A), fission into many small liposomes B), formation of multilamellar vesicles peeling C), bursting D). Classification diversity may...
Model membrane systems revealed that lateral heterogeneity of the mediates localization amyloid beta peptides in a peptide aggregation-dependent manner.
Alternating amphiphilic multiblock molecules 1-4, involving fluorescent hydrophobic units, were designed as mimics for multipass transmembrane proteins. Fluorescence spectroscopy of 1-4 in liposomal membranes suggested the face-to-face stacking units to give folded structures well intermolecular assemblies.
Abstract Liquid–liquid phase separation (LLPS) droplets of biopolymers are known as functional microdroplets in living cells and have recently been used to construct protocells artificial cells. The formation DNA coacervates (also referred droplets) from branched nanostructures the control their physical properties via nanostructure design demonstrated previously. For construction or protocells, however, even though effects such surface tension, wetting, viscoelasticity more important a tiny...
Lateral membrane organization into domains, such as lipid rafts, plays an important role in the selective association of biological and nonbiological materials on heterogeneous surfaces. The localization has profound influence cellular responses. We constructed a biomimetic water-in-oil microdroplet to study lateral these at interfaces. As case study, we studied amyloid β peptide surface. Amyloid attracted much attention one membrane-associating proteins because its "role" Alzheimer's...
Vesicle formation is a fundamental kinetic process related to the vesicle budding and endocytosis in cell. In by artificial means, transformation of lamellar lipid aggregates into spherical architectures key known be prompted e.g. heat, infrared irradiation, alternating electric field induction. Here we report UV-light-driven vesicles from particles consisting crumpled phospholipid multilayer membranes involving photoactive amphiphilic compound composed 1,4-bis(4-phenylethynyl)benzene (BPEB)...
Abstract Bacterial cultivation techniques are classic, basic, and common processes used to characterize the physiological activity of bacteria in their environment. Owing recent advances bacterial techniques, can be elucidated at single‐cell culture level. Here, we report a novel method monitor real‐time growth level inside giant unilamellar vesicles (GUVs). This consists two steps: 1) encapsulation single 1–33 pL scale GUVs 2) immobilization on planar lipid bilayer membrane glass surface....
Abstract Bio‐inspired functional microcapsules have attracted increasing attention in many fields from physical/chemical science to artificial‐cell engineering. Although particle‐stabilised are advantageous for their stability and functionalisation potential, versatile methods desired expand possibilities. This study reports a water‐in‐oil microdroplet stabilised with amphiphilic DNA origami nanoplates. By utilising nanotechnology, nanoplates were designed as nanopore device ion...
We have developed a new method for selectively sorting droplets containing growing bacteria using fluorescence resonance energy transfer (FRET)-based RNA probe. Bacteria and the FRET-based probe are encapsulated into nanoliter-scale droplets, which incubated to allow cell growth. The is cleaved by RNase derived from propagated in resulting an increase intensity. fluorescent distinguishable quenching contain no cells. named this FNAP-sort based on use of nucleic acid bacterial sorting....
Abstract Droplet microfluidics has emerged as a powerful technology for improving the culturing efficiency of environmental microorganisms. However, its widespread adoption been limited due to considerable technical challenges, especially related identification and manipulation individual growth-positive droplets. Here, we combined microfluidic droplet with on-chip “fluorescent nucleic acid probe in droplets bacterial sorting” (FNAP-sort) recovery microdispensing establish an end-to-end...
Cell-sized liposomes are a powerful tool for clarifying physicochemical mechanisms that govern molecular interactions.
Here, we demonstrate a simple method for the rapid production of size-controllable, monodisperse, W/O microdroplets using capillary-based centrifugal microfluidic device. have recently been used in powerful methods that enable miniaturized chemical experiments. Therefore, developing versatile to yield monodisperse is needed. We developed generating based on axisymmetric co-flowing succeeded controlling size by adjusting capillary orifice. Our requires equipment easier-to-use than with other...
Liquid-liquid phase separation (LLPS) droplets of biopolymers are known as functional microdroplets in living cells and have recently been used to construct protocells artificial cells. The formation DNA coacervates (also referred droplets) from branched nanostructures the control their physical properties via nanostructure design were demonstrated previously. For construction or protocells, however, even though effects such surface tension, wetting, viscoelasticity more important a tiny...