Abhijeet Singh

ORCID: 0000-0002-0807-6080
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About
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Research Areas
  • Anaerobic Digestion and Biogas Production
  • Genomics and Phylogenetic Studies
  • Microbial Community Ecology and Physiology
  • Biofuel production and bioconversion
  • Gut microbiota and health
  • Planetary Science and Exploration
  • Microbial Metabolic Engineering and Bioproduction
  • Wastewater Treatment and Nitrogen Removal
  • Geology and Paleoclimatology Research
  • Peatlands and Wetlands Ecology
  • Chromium effects and bioremediation
  • Clostridium difficile and Clostridium perfringens research
  • Innovative Microfluidic and Catalytic Techniques Innovation
  • Fish Biology and Ecology Studies
  • Microbial Fuel Cells and Bioremediation
  • Paleontology and Stratigraphy of Fossils
  • Microbial Metabolism and Applications
  • Hydrological Forecasting Using AI
  • Environmental DNA in Biodiversity Studies
  • Pasture and Agricultural Systems
  • Heavy metals in environment
  • Geochemistry and Elemental Analysis
  • Marine and environmental studies
  • Phosphorus and nutrient management
  • Invertebrate Immune Response Mechanisms

Uppsala University
2022-2024

Swedish University of Agricultural Sciences
2017-2024

Dr. D. Y. Patil Medical College, Hospital and Research Centre
2024

University of Petroleum and Energy Studies
2021

National Bureau of Animal Genetic Resources
2020

Forschungszentrum Jülich
2015

Abstract Propionate is a key intermediate in anaerobic digestion processes and often accumulates association with perturbations, such as elevated levels of ammonia. Under conditions, syntrophic ammonia-tolerant microorganisms play role propionate degradation. Despite their importance, little known about these cross-species interactions. Here, we present metagenomes metatranscriptomic data for novel thermophilic bacteria the partner methanogens enriched propionate-fed reactors. A metagenome...

10.1038/s41396-023-01504-y article EN cc-by The ISME Journal 2023-09-07

The objective was to determine the effects on rumen microbiome and milk quality of reducing methane (CH 4 ) produced from enteric fermentation by addition Asparagopsis taxiformis (AT) diets dairy cows. Six Nordic Red cows at 122 ± 13.7 (mean SD) days in milk, parity 2.7 0.52 producing 36 kg 2.5 per day start trial were divided into three blocks yield assigned an extra-period Latin-square change-over design comprising two dietary treatments. An extra period observation added control for...

10.3389/fanim.2023.1112969 article EN cc-by Frontiers in Animal Science 2023-02-27

Summary To enrich syntrophic acetate‐oxidizing bacteria ( SAOB ), duplicate chemostats were inoculated with sludge from acetate oxidation SAO )‐dominated systems and continuously supplied (0.4 or 7.5 g l −1 ) at high‐ammonia levels. The operated under mesophilic (37°C) thermophilic (52°C) temperature for about six hydraulic retention times HRT 28 days) sampled over time. Irrespective of temperature, a methane content 64–69% effluent level 0.4–1.0 recorded in fed high acetate. Low production...

10.1111/1751-7915.13035 article EN cc-by Microbial Biotechnology 2017-12-14

Summary Inefficient syntrophic propionate degradation causes severe operating disturbances and reduces biogas productivity in many high‐ammonia anaerobic digesters, but propionate‐degrading microorganisms these systems remain unknown. Here, we identified candidate ammonia‐tolerant propionate‐oxidising bacteria using enrichment at high ammonia levels (0.7–0.8 g NH 3 L −1 ) continuously‐fed reactors. We reconstructed 30 high‐quality metagenome‐assembled genomes (MAGs) from the propionate‐fed...

10.1111/1462-2920.15388 article EN cc-by-nc-nd Environmental Microbiology 2021-01-06

The enrichment of CO2-reducing microbial biocathodes is challenging. Previous research has shown that a promising approach could be to first enrich bioanodes and then lower the potential so electrodes are converted into biocathodes. However, effect such transition on community electrode not been studied. goal this study was thus compare start-up from preenriched anodes with direct bare investigate changes in composition. three materials long-term performance also investigated. In study,...

10.1128/aem.02242-17 article EN cc-by Applied and Environmental Microbiology 2017-12-08

The global spread and diversification of multidrug-resistant Gram-negative (MRGN) bacteria poses major challenges to healthcare. In particular, carbapenem-resistant Klebsiella pneumoniae strains have been frequently identified in infections hospital-wide outbreaks. most underlying resistance gene (blaKPC) has spreading over the last decade health care setting. blaKPC seems rapidly diversified found various species on different plasmid types. To review progress dynamics this diversification,...

10.1038/s41598-019-47758-5 article EN cc-by Scientific Reports 2019-08-02

Abstract Climate change has been intensifying soil drying and rewetting cycles, which can alter the microbiome structure activity. Here we hypothesized that a drying-rewetting cycle enhances biodegradation and, hence, decreases effectiveness of nitrification inhibitors (NIs). The DMPP (3,4-Dimethylpyrazole phosphate) MP + TZ (3-Methylpyrazol Triazol) was evaluated in 60-day incubation studies under relative to constant low high moisture conditions (40% 80% water-holding capacity, WHC,...

10.1007/s00374-024-01811-2 article EN cc-by Biology and Fertility of Soils 2024-03-15

This study evaluated the effect of whole and autolysed Yarrowia lipolytica CBS 7504 strain on growth performance, gut immunity microbiota composition in juvenile rainbow trout (Oncorhynchus mykiss). Fish were fed diets containing 2% 5% or Y. (WY AY) a 45-day trial, with no-yeast diet as control. Log2 fold change analysis showed relative abundance association certain microbial taxa respect to control such correlation between Desulfovibrionaceae WY. Moreover, at levels demonstrated synergistic...

10.1016/j.aquaculture.2024.741065 article EN cc-by Aquaculture 2024-05-09

Multiple measurements can be used to quantify the carbon dioxide (CO2) captured during enhanced rock weathering (ERW) applications. In most terrestrial applications, CO2 is dominantly stored as carbonate alkalinity inside water. Total (TA) often taken a measure of in However, contribution acids other than carbonic acid mineral dissolution and, thus, generated must studied closely reliably capture. In this study, we test how dissolved organic (DOC) impacts non-carbonate and charge...

10.5194/egusphere-egu25-18005 preprint EN 2025-03-15

<ns3:p>Background The optimization of enhanced mineral weathering as a carbon dioxide removal technology requires comprehensive understanding what drives weathering. These drivers can be abiotic and biotic interact with each other. Therefore, in this study, an extensive 8-week column experiment was set up to investigate 29 potential simultaneously. Methods setup included various combinations types surface areas, irrigation settings, biochar organic amendments, along biota products such...

10.12688/openreseurope.19252.1 article EN cc-by Open Research Europe 2025-03-18

Microbial inhibition by high ammonia concentrations is a recurring problem that significantly restricts methane formation from intermediate acids, i.e., propionate and acetate, during anaerobic digestion of protein-rich waste material. Studying the syntrophic communities perform acid conversion challenging, due to their relatively low abundance within microbial typically found in biogas processes disruption cooperative behavior pure cultures. To overcome these limitations, this study...

10.3389/fmicb.2024.1389257 article EN cc-by Frontiers in Microbiology 2024-06-05

Abstract Acetogenic bacteria are imperative to environmental carbon cycling and diverse biotechnological applications, but their extensive physiological taxonomical diversity is an impediment systematic taxonomic studies. Acetogens chemolithoautotrophic that perform reductive fixation under anaerobic conditions through the Wood–Ljungdahl pathway (WLP)/acetyl-coenzyme A pathway. The gene-encoding formyltetrahydrofolate synthetase (FTHFS), a key enzyme of this pathway, highly conserved can be...

10.1093/database/baz142 article EN cc-by-nc Database 2019-01-01

Abstract Acetogens play a key role in anaerobic degradation of organic material and maintaining biogas process efficiency. Profiling this community its temporal changes can help evaluate stability function, especially under disturbance/stress conditions, avoid complete failure. The formyltetrahydrofolate synthetase (FTHFS) gene be used as marker for acetogenic profiling diverse environments. In study, we developed new high-throughput FTHFS sequencing method compared it with conventional...

10.1038/s41598-021-92658-2 article EN cc-by Scientific Reports 2021-06-24

Phase contrast microscopy cannot give sufficient information on bacterial metabolic activity, or if a cell is dead, it has the fate to die in viable but non-growing state. Thus, reliable sensing of activity helps distinguish different categories viability. We present non-invasive instantaneous method using fluorogenic substrate for online monitoring esterase and calcein efflux changes growing wild type bacteria. The fluorescent conversion product acetoxymethyl ester (CAM) its indicates cells...

10.1371/journal.pone.0141768 article EN cc-by PLoS ONE 2015-10-29

Gut dysbiosis has been implicated in the pathophysiology of a growing number non-communicable diseases. High through-put sequencing technologies and short chain fatty acid (SCFA) profiling enables surveying composition function gut microbiota provide key insights into host-microbiome interactions. However, methodological problem with analyzing stool samples is that are treated stored differently prior to submission for analysis potentially influencing its metabolites. In present study, we...

10.1371/journal.pone.0236944 article EN cc-by PLoS ONE 2020-08-03

An anaerobic bacterial strain, designated AMB_01

10.1099/ijsem.0.005328 article EN INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 2022-03-21

Complete edentulism significantly affects the esthetics, function, and quality of life elderly. Implant-supported hybrid dentures are superior to conventional removable prostheses in terms stability, masticatory function. This case report details dental rehabilitation a 75-year-old female patient seeking total restoration. Clinical evaluation revealed partial both arches accompanied by residual root stumps. Eight endosseous implants were placed maxilla seven mandible following delayed...

10.7759/cureus.71399 article EN Cureus 2024-10-13

The formyltetrahydrofolate synthetase (FTHFS) gene is a molecular marker of choice to study the diversity acetogenic communities. However, current analyses are limited due lack high-throughput sequencing approach for FTHFS amplicons and dedicated bioinformatics pipeline data analysis, including taxonomic annotation visualization sequence data. In present study, we combined barcode multiplexed with unsupervised analysis visualize community structure. We used samples from biogas digester...

10.3389/fmicb.2020.02066 article EN cc-by Frontiers in Microbiology 2020-08-27

Acetogens play a very important role in anaerobic digestion and are essential ensuring process stability. Despite this, targeted studies of the acetogenic community biogas processes remain limited. Some efforts have been made to identify understand this community, but lack reliable molecular analysis strategy makes detection bacteria tedious. Recent suggest that screening bacterial genetic material for formyltetrahydrofolate synthetase (FTHFS), key marker enzyme Wood-Ljungdahl pathway, can...

10.3389/fmicb.2021.700256 article EN cc-by Frontiers in Microbiology 2021-08-16

Cattle manure has a low energy content and high fibre water content, limiting its value for biogas production. Co-digestion with more energy-dense material can improve the output, but co-substrate composition that gives best results in terms of degree degradation, gas production digestate quality not yet been identified. This study examined effects carbohydrate, protein fat as co-substrates from cattle manure. Laboratory-scale semi-continuous mesophilic reactors were operated mono-digestion...

10.1111/1751-7915.14194 article EN cc-by-nc-nd Microbial Biotechnology 2022-12-12

Abstract Lava tubes on Mars hold exciting potential for the preservation of biosignatures, which may survive geological timescales in these isolated, stable environments. To support development future astrobiological mission concepts, we turn to terrestrial lava tubes, host a variety microbial communities and secondary minerals. Following multidisciplinary sampling protocol, retrieved biological, molecular, mineralogical data from several Iceland. We report blue‐colored copper‐rich minerals...

10.1029/2022ea002234 article EN cc-by Earth and Space Science 2022-04-15
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