A5036120274- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Hydrogels: synthesis, properties, applications
- Mesenchymal stem cell research
- Nanoplatforms for cancer theranostics
- Neuroinflammation and Neurodegeneration Mechanisms
- Graphene and Nanomaterials Applications
- Neuroscience and Neural Engineering
- Tissue Engineering and Regenerative Medicine
- Spinal Cord Injury Research
- Bone Tissue Engineering Materials
- Electrospun Nanofibers in Biomedical Applications
- Pluripotent Stem Cells Research
- RNA Research and Splicing
- Glioma Diagnosis and Treatment
- Advanced Drug Delivery Systems
- Axon Guidance and Neuronal Signaling
- Retinal and Macular Surgery
- Advanced Polymer Synthesis and Characterization
- Intraocular Surgery and Lenses
- Optical Coherence Tomography Applications
- Advanced Sensor and Energy Harvesting Materials
- Cellular Mechanics and Interactions
- Osteoarthritis Treatment and Mechanisms
- RNA Interference and Gene Delivery
Boston University
2022-2025
University of California, Los Angeles
2016-2024
University of California System
2019
California NanoSystems Institute
2017
Massachusetts Institute of Technology
2010-2017
UCLA Health
2017
Geisinger Neuroscience Institute
2015
Geisinger Medical Center
2015
TiVo (United States)
2015
New England Baptist Hospital
2015
Central nervous system (CNS) lesions become surrounded by neuroprotective borders of newly proliferated reactive astrocytes; however, fundamental features these cells are poorly understood. Here we show that following spinal cord injury or stroke, 90% and 10% border-forming astrocytes derive, respectively, from proliferating local oligodendrocyte progenitor in adult mice both sexes. Temporal transcriptome analysis, single-nucleus RNA sequencing immunohistochemistry after focal CNS injury,...
Abstract Ependyma have been proposed as adult neural stem cells that provide the majority of newly proliferated scar-forming astrocytes protect tissue and function after spinal cord injury (SCI). This proposal was based on small, midline stab SCI. Here, we tested generality this by using a genetic knock-in cell fate mapping strategy in different murine SCI models. After large crush injuries across entire cord, ependyma-derived progeny remained local, did not migrate contributed few any kind...
Graphical Abstract A transesterfication reaction is used to synthesize tri-thiol-functionalized-ethoxylated polyols that are combined with polyethylene glycol diacrylates form a biodegradable hydrogel library. Hydrogels display nonswelling equilibration and offer temporal control over material degradation the release of biomolecules. The demonstrated in vitro biocompatibility makes this versatile platform can be for local drug delivery volume-constrained anatomical sites.
Synthetic diblock copolypeptides were designed to incorporate oppositely charged ionic segments that form β-sheet-structured hydrogel assemblies via polyion complexation when mixed in aqueous media. The observed chain conformation directed assembly was found be required for efficient formation and provided distinct useful properties these hydrogels, including self-healing after deformation, microporous architecture, stability against dilution While many promising self-assembled materials...
Abstract Biomaterials hold promise for therapeutic applications in the central nervous system (CNS). Little is known about molecular factors that determine CNS foreign body responses (FBRs) vivo, or how such influence biomaterial function. Here, we probed these mice using a platform of injectable hydrogels readily modified to present interfaces with different physiochemical properties host cells. We found FBRs mimic specialized multicellular wound not peripheral tissues, which serve isolate...
Abstract Neural progenitor cells (NPC) represent potential cell transplantation therapies for CNS injuries. To understand how lesion environments influence transplanted NPC fate in vivo, we derived expressing a ribosomal protein-hemagglutinin tag (RiboTag) transcriptional profiling of NPC. Here, show that grafted into uninjured mouse generate are transcriptionally similar to healthy astrocytes and oligodendrocyte lineages. In striking contrast, subacute lesions after stroke or spinal cord...
Abstract Neural tissue damaged after central nervous system (CNS) injury does not naturally regenerate but is instead replaced by non‐neural fibrotic scar that serves no neurological function. Scar‐free repair to create a more permissive environment for regeneration requires altering the natural responses of glial cells. In this work, glycopolymer‐based supramolecular hydrogels are synthesized direct adaptive glia CNS injury. Combining poly(trehalose‐ co ‐guanosine) (pTreGuo) glycopolymers...
Precise regulation of Egr2 transcription is fundamentally important to the control peripheral myelination. Here, we describe a long non-coding RNA antisense promoter (Egr2-AS-RNA). During nerve injury, expression Egr2-AS-RNA increased and correlates with decreased transcript protein levels. Ectopic in dorsal root ganglion (DRG) cultures inhibits mRNA induces demyelination. In vivo inhibition using oligonucleotide GapMers released from biodegradable hydrogel following sciatic injury reverts...
Abstract Residual mechanical stresses, also known as solid emerge during rapid differential growth or remodeling of tissues, observed in morphogenesis and tumor growth. While residual stresses typically dissipate most healthy adult organs, the rate decreases, high have been reported mature, brains. However, origins consequences brain across health, aging, disease remain poorly understood. Here, we utilized validated a previously developed method to map brains mice three age groups: 5–7 days,...
Abstract Central nervous system (CNS) lesions become surrounded by neuroprotective borders of newly proliferated reactive astrocytes. Fundamental features these cells are poorly understood. Here, we show that 90% border-forming astrocytes derive from proliferating Aldh1l1-expressing local astrocytes, and 10% Pdgfra-expressing oligodendrocyte progenitors in mice. Temporal transcriptome analysis, snRNAseq immunohistochemistry showed after CNS injury, mature dedifferentiated, proliferated,...
Hydrogels with covalently incorporated trehalose are synthesized using thiol-ene Michael addition. Trehalose hydrogels afford prolonged stabilization and controlled release of model enzymes in vitro vivo as well preservation protein stability under heat lyophilization stressors. Strong ordered hydrogen bonding interactions within represent a possible mechanism for stabilization. As service to our authors readers, this journal provides supporting information supplied by the authors. Such...