A5016566078- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Nicotinic Acetylcholine Receptors Study
- Receptor Mechanisms and Signaling
- Biochemical and Structural Characterization
- Chemical Synthesis and Analysis
- Cell Adhesion Molecules Research
- SARS-CoV-2 and COVID-19 Research
- Ocean Acidification Effects and Responses
- Cancer Genomics and Diagnostics
- Ion channel regulation and function
- Antimicrobial Peptides and Activities
- CAR-T cell therapy research
- bioluminescence and chemiluminescence research
- Physiological and biochemical adaptations
- Cancer Cells and Metastasis
- Neurotransmitter Receptor Influence on Behavior
- Marine Bivalve and Aquaculture Studies
- Neurobiology and Insect Physiology Research
- Angiogenesis and VEGF in Cancer
- Carbohydrate Chemistry and Synthesis
- Respiratory viral infections research
- Cancer, Hypoxia, and Metabolism
Center for Scientific Research and Higher Education at Ensenada
2016-2025
Introduction This study explores the analgesic potential of novel conotoxin O1_cal6.4b, derived from Californiconus californicus , as a candidate for pain management in model inflammatory pain. Methods O1_cal6.4b was systemically administered to Wistar rats, and its effects on thermal hyperalgesia motor coordination were evaluated. Comparative analyses conducted against O1_cal6.4d, ω -MVIIA, standard analgesics (morphine, dexamethasone, diclofenac). Structural differences between O1_cal6.4d...
The variable domain of New Antigen Receptors (vNAR) from sharks, present special characteristics in comparison to the conventional antibody molecules such as: small size (12–15 kDa), thermal and chemical stability great tissue penetration, that makes them a good alternative source as therapeutic or diagnostic agents. Therefore, it is essential improve techniques used for development selection vNAR antibodies recognize distinct antigens. synthetic libraries offers fast option generation with...
Abstract Immunotherapies based on antibody fragments have been developed and applied to human diseases, describing novel formats. The vNAR domains a potential therapeutic use related their unique properties. This work used non-immunized Heterodontus francisci shark library obtain with recognition of TGF-β isoforms. isolated T1 selected by phage display demonstrated binding the isoforms (-β1, -β2, -β3) direct ELISA assay. These results are supported using for first time Single-Cycle kinetics...
Severe Acute Respiratory Syndrome Coronavirus 2 is the causal pathogen of coronavirus disease 2019 (COVID-19). The emergence new variants with different mutational patterns has limited therapeutic options available and complicated development effective neutralizing antibodies targeting spike (S) protein. Variable New Antigen Receptors (VNARs) constitute a antibody technology that been introduced into list possible against SARS-CoV-2. unique qualities VNARs, such as high affinities for target...
Californiconus californicus, previously named Conus has always been considered a unique species within cone snails, because of its molecular, toxicological and morphological singularities; including the wide range diet, since it is capable preying indifferently on fish, octopus, shrimps, worms. We report here new cysteine pattern conotoxin assigned to O1-superfamily inhibiting growth Mycobacterium tuberculosis (Mtb). The was tested pathogen reference strain (H37Rv) multidrug-resistant...
Small peptides isolated from the venom of marine snails belonging to genus Conus have been largely studied because their therapeutic value. These can be classified in two groups. The largest one is composed by rich disulfide bonds, and referred as conotoxins. Despite importance conotoxins given pharmacology value, little known about protein isomerase (PDI) enzymes that are required catalyze correct folding. To discover PDIs may participate folding structural maturation conotoxins,...
Lung cancer is one of the most common types cancer, accounting for approximately 15% all cases worldwide. Apoptosis dominant defense mechanism against tumor development. The balance between pro- and antiapoptotic members Bcl-2 protein family can determine cellular fate. venom predatory marine snails Conus estimated to have 100–400 toxins called conotoxins. α-conotoxins known consist selective antagonists nicotinic acetylcholine receptors (nAChRs). cells overexpress several subunits nAChRs...
Research into various proteins capable of blocking metabolic pathways has improved the detection and treatment multiple pathologies associated with malfunction overexpression different metabolites. However, antigen-binding have limitations. To overcome disadvantages available proteins, present investigation aims to provide chimeric peptides by binding a complementarity-determining region 3 (CDR3) variable domains new antigen receptors (VNARs) conotoxin. Six non-natural antibodies...
The stability, binding, and tissue penetration of variable new-antigen receptor (VNAR) single-domain antibodies have been tested as part an investigation into their ability to serve novel therapeutics. V13 is a VNAR that recognizes vascular endothelial growth factor 165 (VEGF165). In the present study was used parental molecule which we introduced mutations designed in silico. Two mutants were expressed, recognize VEGF165 assessed vitro vivo. One mutation (Pro98Tyr) increase recognition,...
We investigated three species of Conidae, one on the western shores Baja California (Californiconus californicus), and two in Gulf (Conasperella perplexus Conasperella ximenes). In a thermal horizontal gradient trial with day–night cycles, we determined final preference snails. Californiconus californicus had preferred average temperature 23.3 °C, compared 22.7 °C for Co. whereas ximenes diel 20.1 during day 27.2 at night. The displacement velocity Ca. was 12.6 ± 1.9 cm h−1 versus 17.6 2...
Abstract After arriving in bone, cancer cells can enter a dormancy state that last for years, during which they are resistant to treatment. The bone microenvironment induces via mechanisms similar the ones of hematopoietic stem (HSC). angiopoietin receptor, TIE2, HSC and some is target anti-angiogenic treatments. However, its inhibition may restart proliferation cells, posing risk metastasis growth, while increasing sensitivity chemotherapy. Thus, we aim characterize role TIE2 metastases. We...