A5100662125- T-cell and B-cell Immunology
- Atmospheric chemistry and aerosols
- Immune Cell Function and Interaction
- Vector-borne infectious diseases
- Air Quality Monitoring and Forecasting
- CAR-T cell therapy research
- Viral Infections and Vectors
- Immunotherapy and Immune Responses
- Leptospirosis research and findings
- Atmospheric and Environmental Gas Dynamics
- Galectins and Cancer Biology
- Advancements in Semiconductor Devices and Circuit Design
- Cytomegalovirus and herpesvirus research
- Toxin Mechanisms and Immunotoxins
- Nanowire Synthesis and Applications
- Lymphoma Diagnosis and Treatment
- CRISPR and Genetic Engineering
- Molecular Biology Techniques and Applications
- Odor and Emission Control Technologies
- Genomics and Chromatin Dynamics
- Orthopedic Infections and Treatments
- Bacterial Infections and Vaccines
- Water Quality Monitoring and Analysis
- Renal Transplantation Outcomes and Treatments
- Endoplasmic Reticulum Stress and Disease
National University of Singapore
2018-2022
Hunan Children's Hospital
2013
National Cancer Institute
2002-2004
National Institutes of Health
1997-2004
Center for Cancer Research
2004
University Health Network
2002
University of Toronto
1998-2002
Toronto General Hospital
1998-1999
University of Utah
1992-1997
National Institute of Allergy and Infectious Diseases
1997
In human Lyme disease, symptoms with widely varying levels of severity have been observed. A mouse model disease has developed which allows analysis mice mild, moderate, and severe pathologies after inoculation the spirochete Borrelia burgdorferi. To determine whether differences in reflect number spirochetes persisting affected tissues, a sensitive PCR technique was to detect B. burgdorferi DNA virtually any tissue an infected mouse. This analysis, detects from as few three spirochetes,...
Current approved chimeric antigen receptor (CAR) T-cell products are autologous cell therapies that costly and poorly accessible to patients. We aimed evaluate the safety antitumor activity of a novel off-the-shelf anti-CD19 CAR-engineered allogeneic double-negative T cells (RJMty19) in patients with relapsed/refractory large B-cell lymphoma. report results from first-in-human, open-label, single-dose, phase 1 study CD19-specific CAR (CAR-DNT) cells.
Borrelia burgdorferi produces a mitogen for murine B lymphocytes which can be measured in vitro by polyclonal stimulation of proliferation and immunoglobulin production (R. Schoenfeld, B. Araneo, Y. Ma, L. Yang, J. Weis, Infect. Immun. 60:455-464, 1992). Sonicated cells also stimulated IL-6 splenocyte cultures. We have used the model Lyme disease described Barthold et al. (S. W. Barthold, D. S. Beck, G. M. Hansen, A. Terwilliger, K. Moody, Dis. 162:133-138, 1990) to determine whether B-cell...
Lyme disease refers to the multisymptomatic illness in humans which results from infection with tick-borne spirochete Borrelia burgdorferi. The white-footed mouse is major reservoir for B. burgdorferi and, upon infection, certain inbred mice develop symptoms similar those reported human disease. Sonicated preparations of washed spirochetes were found have potent mitogenic activity when cultured lymphocytes naive C57BL/6, C3H/HeJ, or BALB/c mice. sonicate was approximately fourfold greater...
Abstract The regulation of apoptosis in mature CD4+ or CD8+ αβ+ T cells has been well studied. How the survival and death is regulated peripheral CD4−CD8− (double negative, DN) remains unknown. Recent studies suggest that DN may play an important role immune responses mediated by cells. Here, we used immunosuppressive cell clones to elucidate mechanisms involved were generated from spleen 2C transgenic mice, which express TCR specific for Ld permanently accepted Ld+ skin allografts after...
The clinical manifestations of human Lyme disease present with a spectrum tissue or organ involvement and severity symptoms. murine model has proved to be an accurate reflection many the symptoms been particularly useful for studying development subacute arthritis tendonitis. Direct invasion by Borrelia burgdorferi persistence high levels spirochetes in tissues are important components development. outer-surface lipoproteins contain biologically active lipid-modified moiety potent ability...
Abstract Objectives To evaluate the manufacturability, efficacy and safety of allogeneic CD19 chimeric antigen receptor double‐negative T cells (CD19‐CAR‐DNTs) as an off‐the‐shelf therapeutic cell product. Methods A membrane proteome array was used to assess off‐target binding CD19‐CAR. DNTs derived from healthy donors were transduced with lentiviral vectors encoding The manufacture CD19‐CAR‐DNTs under GMP conditions, their surface molecule expression patterns characterised using flow...
Abstract This study was intended to delineate the profile of double‐negative T cells (DNTs) in NOD.Cg‐Prkdc scid Il2rg tm1wj /SzJ mice and cytokines released from DNTs vivo vitro. Total 4 × 10 7 RC1012 injection per were intravenously infused. IFN‐γ, TNF‐α, IL‐1β, IL‐2, IL‐4, IL‐6, IL‐10 measured A quantitative polymerase chain reaction (PCR) employed determine gene copies Notch2‐NLA DNT cell collected organs. Cytokines significantly increased vitro (4 h) (3 h). distributed into lung, liver,...
Given the increasing importance of effectively identifying synergistic changes between PM2.5 and O3 comprehensively analyzing their impact on air quality management in China, we employ Sen+Mann–Kendall (Sen+M-K) trend test this study to examine temporal spatial variation trends Yangtze River Delta (YRD), from 2003 2020. We identified regions where these pollutants exhibited established pathways potential drivers, using geographically weighted random forest algorithms structural equation...
Abstract. Aerosol pH is often calculated based on different standard states thus making it inappropriate to compare aerosol acidity parameters derived thereby. Such comparisons are however routinely performed in the atmospheric science community. This study attempts address this issue for first time by providing a theoretical framework and convert between values molarity, molality mole fractions. Using hourly ionic species measurements Guangzhou, China, observed that pHx (mole fraction...
Objective The study was conducted to investigate the acute phase of convulsion related problem on clinical manifestations,imaging and electroencephalograph (EEG) examination purulent meningitis.Methods Cluster sampling method employed select children in our hospital,a total 301 cases with meningitis analyzed retrospectively.Among them,62 had convulsion.The incidence meningitis,risk factors,and prognosis were analyzed.Results rate 20.60％.The partial seizure eight (12.90％).The secondarily...
Abstract. Methylamines can readily react with acidic gases in the atmosphere, which consequently has an important impact on atmospheric environment. It is difficult to measure amines field studies due their high reactivity, and therefore, numerical modelling effective tool study ambient amines. However, contribution of marine biological emissions (MBE), source methylamines (MA), been insufficiently investigated current emission inventory. Therefore, this utilized satellite data such as Sea...