A5056694848- PARP inhibition in cancer therapy
- Click Chemistry and Applications
- Chemical Synthesis and Analysis
- Integrated Circuits and Semiconductor Failure Analysis
- CRISPR and Genetic Engineering
- Histone Deacetylase Inhibitors Research
- Protein Degradation and Inhibitors
- Epigenetics and DNA Methylation
- Biotin and Related Studies
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- Antimicrobial Peptides and Activities
- Cell death mechanisms and regulation
- Protein Structure and Dynamics
- Toxin Mechanisms and Immunotoxins
- Viral Infectious Diseases and Gene Expression in Insects
University of California, Santa Cruz
2015-2024
Oregon Health & Science University
2018-2023
Child Trends
2022
Poly(ADP-ribose) polymerase 7 (PARP-7) has emerged as a critically important member of large enzyme family that catalyzes ADP-ribosylation in mammalian cells. PARP-7 is critical regulator the innate immune response. What remains unclear mechanism by which regulates this process, namely because protein targets mono-ADP-ribosylation (MARylation) are largely unknown. Here, we combine chemical genetics, proximity labeling, and proteome-wide amino acid site profiling for identifying direct sites...
The effect of peptide-to-peptoid substitutions on the passive membrane permeability an N-methylated cyclic hexapeptide is examined. In general, maintained but increased conformational heterogeneity. Diversification with nonproteinogenic side chains up to 3-fold. Additionally, impact peptoid within a β-turn are explored. Based these results, strategic incorporation residues into peptides can maintain or improve cell permeability, while increasing access diverse side-chain functionality.
Abstract Histone deacetylases (HDACs) play a crucial role in transcriptional regulation and are implicated various diseases, including cancer. They involved histone tail deacetylation canonically linked to repression. Previous studies suggested that HDAC recruitment cell-cycle gene promoters via the retinoblastoma (RB) protein or DREAM complex through SIN3B is essential for G1/S G2/M repression during arrest exit. Here we investigate interplay among DREAM, RB, SIN3 proteins, HDACs context of...
Poly-ADP-ribose polymerases (PARPs 1–16) have emerged as major regulators of diverse cellular processes. PARPs can be subclassified based on their ability to catalyze poly-ADP-ribosylation (PARylation) or mono-ADP-ribosylation (MARylation). While much is known about the roles that PARylation (e.g., PARP1), function MARylation PARP10) substantially less understood. This due in large part lack small-molecule inhibitors are selective for individual PARP family members MARylation. Herein, we...
Controlled protein synthesis is required to regulate gene expression and often carried out in a cell type-specific manner. Protein commonly measured by labeling the nascent proteome with amino acid analogs or isotope-containing acids. These methods have been difficult implement vivo as they require lengthy replacement procedures. O-propargyl-puromycin (OPP) puromycin analog that incorporates into polypeptide chains. Through its terminal alkyne, OPP can be conjugated fluorophore-azide for...
Histone deacetylases (HDACs) are pivotal in transcriptional regulation, and their dysregulation has been associated with various diseases including cancer. One of the critical roles HDAC-containing complexes is deacetylation histone tails, which canonically linked to repression. Previous research indicated that HDACs recruited cell-cycle gene promoters through RB protein or DREAM complex via SIN3B HDAC activity essential for repressing G1/S G2/M genes during arrest exit. In this study, we...
ABSTRACT Controlled protein synthesis is required to regulate gene expression and often carried out in a cell type-specific manner. Protein commonly measured by labeling the nascent proteome with amino acid analogs or isotope-containing acids. These methods have been difficult implement vivo as they require lengthy replacement procedures. O-propargyl-puromycin (OPP) puromycin analog that incorporates into polypeptide chains. Through its terminal alkyne, OPP can be conjugated...