A5039897897- Nerve injury and regeneration
- PARP inhibition in cancer therapy
- Neuroscience of respiration and sleep
- Neuroscience and Neural Engineering
- RNA modifications and cancer
- Neuroscience and Neuropharmacology Research
- Neurobiology and Insect Physiology Research
- Insect Resistance and Genetics
- Cancer-related Molecular Pathways
- Molecular Biology Techniques and Applications
- Pain Mechanisms and Treatments
- Cellular transport and secretion
- Free Radicals and Antioxidants
- Toxin Mechanisms and Immunotoxins
- Genetics and Neurodevelopmental Disorders
- Biochemical Analysis and Sensing Techniques
- Metabolism, Diabetes, and Cancer
- Calcium signaling and nucleotide metabolism
- CRISPR and Genetic Engineering
- Electrostatic Discharge in Electronics
- Physiological and biochemical adaptations
- Neuropeptides and Animal Physiology
- Phosphodiesterase function and regulation
- Neuroendocrine regulation and behavior
- Studies on Chitinases and Chitosanases
Oregon Health & Science University
2011-2024
VA Portland Health Care System
2020-2024
Poly(ADP-ribose) polymerase 14 (PARP14) is a member of the PARP family enzymes that transfer ADP-ribose from NAD+ to nucleophilic amino acids on target proteins, process known as mono-ADP-ribosylation (MARylation). PARP14 involved in normal immune function through IL-4 signaling pathway and prosurvival factor multiple myeloma hepatocellular carcinoma. A mechanistic understanding physiological pathophysiological roles has been limited by dearth PARP14-specific MARylation targets. Herein we...
Abstract The three Drosophila atypical soluble guanylyl cyclases, Gyc-89Da, Gyc-89Db, and Gyc-88E, have been proposed to act as oxygen detectors mediating behavioral responses hypoxia. larvae mutant in any of these subunits were defective their hypoxia escape response—a rapid cessation feeding withdrawal from food. This response required cGMP the cyclic nucleotide-gated ion channel, cng, but did not appear be dependent on either cGMP-dependent protein kinases, dg1 dg2. Specific activation...
Accumulating evidence underscores the importance of ligand-receptor dynamics in shaping cellular signaling. In nervous system, growth factor-activated Trk receptor trafficking serves to convey biochemical signaling that underlies fundamental neural functions. Focus has been placed on axonal but little is known about neuronal soma, particularly at molecular scale, due large part technical hurdles observing individual factor-Trk complexes for long periods time inside live cells. Quantum dots...
Poly-ADP-ribose polymerases (PARPs 1–16) have emerged as major regulators of diverse cellular processes. PARPs can be subclassified based on their ability to catalyze poly-ADP-ribosylation (PARylation) or mono-ADP-ribosylation (MARylation). While much is known about the roles that PARylation (e.g., PARP1), function MARylation PARP10) substantially less understood. This due in large part lack small-molecule inhibitors are selective for individual PARP family members MARylation. Herein, we...
Abstract Mono-ADP-ribosylation (MARylation) of mammalian proteins was first described as a post-translational modification catalyzed by bacterial toxins. It is now known that endogenous MARylation occurs in cells and 11 members the poly-ADP-ribose polymerase (PARP) family (17 humans). The physiological roles these PARPs remain largely unknown. Here we demonstrate PARP6, neuronally enriched PARP catalyzes MARylation, regulates hippocampal dendrite morphogenesis, process critical for proper...
Abstract Activity of arterial baroreceptors is modulated by neurohumoral factors, including nitric oxide (NO), released from endothelial cells. Baroreceptor reflex responses can also be NO signaling in the brainstem nucleus tractus solitarius (NTS), primary central target cardiovascular afferents. Our recent studies indicate that brain‐derived neurotrophic factor (BDNF) abundantly expressed developing and adult baroreceptor afferents vivo, cultured nodose ganglion (NG) neurons patterns...
Background . We investigated the effects of sulforaphane (SF), main active isothiocyanate in cruciferous vegetables, on arachidonic acid (AA) metabolism kidney and its effect arterial blood pressure, using spontaneously hypertensive rats (SHR) as models. Methods Rats were treated for 8 weeks with either drinking water alone (control) or SF (20 40 mg/kg) added to water. Mean pressure (MAP) was measured at 7-day intervals throughout study. At end treatment euthanized, kidneys harvested prepare...
Abstract Hypertension leads to structural and functional changes at baroreceptor synapses in the medial nucleus tractus solitarius (NTS), but underlying molecular mechanisms remain unknown. Our previous studies show that brain‐derived neurotrophic factor (BDNF) is abundantly expressed by rat nodose ganglion (NG) neurons, including afferents their central terminals NTS. We hypothesized hypertension upregulation of BDNF expression NG neurons. To test this hypothesis, we used two...
Metastasis is the most dreaded outcome after a breast cancer diagnosis, and little known regarding what triggers or promotes to spread distally, how prevent eradicate metastasis effectively. Bilateral cancers are an uncommon form of cancers. In our study, percentage bilateral were clonally related based on copy number variation profiling. Whole exome sequencing comparative sequence analysis revealed that limited somatic mutations acquired in this “breast-to-breast” might promote distant...
// Anke Vermehren-Schmaedick 1 , 2 3 Paulette Mhawech-Fauceglia 4 Byung S. Park 5 6 Tanja Pejovic 7 * and Shiuh-Wen Luoh Hospital & Specialty Medicine, VA Portland Health Care System, Portland, OR, USA Translational Oncology Program, Knight Cancer Institute, Oregon Science University, Division of Hematology Medical Oncology, Department Anatomic Pathology, Aurora Diagnostics/Sonic Healthcare, Las Vegas, NV, Biostatistics Shared Resource, School Public Health, University - State Gynecologic...
Pde1c is a calcium/calmodulin-regulated, dual-specificity cyclic nucleotide phosphodiesterase. We have used transposon insertion line to investigate the physiological function of in Drosophila melanogaster and show that leads male sterility mating behavior defects include reduced copulation rates. Sterility appears be primarily due elimination sperm from female reproductive system. The were fully rescued by expression exogenous under control either or pan-neuronal promoter, whereas could...
PARP6, a member of family enzymes (17 in humans) known as poly-ADP-ribose polymerases (PARPs), is neuronally enriched PARP. While previous studies from our group show that Parp6 regulator dendrite morphogenesis rat hippocampal neurons, its function the nervous system vivo poorly understood. Here, we describe generation loss-of-function mouse model for examining during neurodevelopment vivo. Using CRISPR-Cas9 mutagenesis, generated line expressed truncated variant (Parp6TR) place Parp6WT....
Retrograde neurotrophin (NT) transport is a specialized form of signal transduction used to conduct information from axons the cell bodies central and peripheral nervous system neurons. It activated upon NT-Trk receptor binding, internalization into signaling endosomes, their motion along axon toward body. Brain-derived neurotrophic factor (BDNF) an abundant NT that modulates key brain spinal cord functions, defects in BDNF trafficking are associated with neuronal death, neurodegenerative...
Abstract Objectives Growth factor receptor bound protein 7 (GRB7) is a multidomain signaling adaptor. Members of the Grb7/10/14 family, specifically Gbrb10/14, have important roles in metabolism. We ablated Grb7 gene mice to examine its metabolic function. Methods Global ablation FVB/NJ was generated. Growth, organ weight, food intake, and glucose homeostasis were measured. Insulin examined by Western blotting. Fat lean body mass measured nuclear magnetic resonance, composition after fasting...