A5044379193- PARP inhibition in cancer therapy
- Immune Cell Function and Interaction
- Signaling Pathways in Disease
- interferon and immune responses
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Plant tissue culture and regeneration
- Neuropeptides and Animal Physiology
- Plant Parasitism and Resistance
- Immune Response and Inflammation
- Toxin Mechanisms and Immunotoxins
- Pharmacological Receptor Mechanisms and Effects
- Click Chemistry and Applications
- Research on Leishmaniasis Studies
- Toxoplasma gondii Research Studies
- Neurological diseases and metabolism
- RNA and protein synthesis mechanisms
- Integrated Circuits and Semiconductor Failure Analysis
- Protein Tyrosine Phosphatases
- Tryptophan and brain disorders
- Electrostatic Discharge in Electronics
- Chemical Synthesis and Analysis
- Cytokine Signaling Pathways and Interactions
- Chronic Kidney Disease and Diabetes
- Cardiac Fibrosis and Remodeling
Oregon Health & Science University
2014-2022
Veterans Health Administration
2020
Vollum Institute
2016
Yonsei University
2015
Heilongjiang Provincial Hospital
2014-2015
Hangzhou Normal University
2013
Zhejiang Gongshang University
2011-2013
A diverse array of microbial metabolites binds to MR1 and selectively activates MR1-restricted T cells.
Adenosine diphosphate ribosyltransferases (ARTDs; ARTD1-17 in humans) are emerging as critical regulators of cell function both normal physiology and disease. These enzymes transfer the ADP-ribose moiety from its substrate, nicotinamide adenine dinucleotide (NAD(+)), to amino acids target proteins. The functional redundancy overlapping specificities among 17 ARTDs humans make identification direct targets individual ARTD family members a cellular context formidable challenge. Here we...
ADP-ribosyltransferases (ARTD1–16) have emerged as major downstream effectors of NAD+ signaling in the cell. Most ARTDs (ARTD7 and 8, 10–12, 14–17) catalyze transfer a single unit ADP-ribose from to target proteins, process known mono-ADP-ribosylation (MARylation). Progress understanding cellular functions MARylation has been limited by inability identify direct targets for individual mono-ARTDs. Here, we engineered mono-ARTDs use an analog that is orthogonal wild-type ARTDs. We profiled...
Article29 October 2019Open Access Transparent process 4′-Phosphopantetheine corrects CoA, iron, and dopamine metabolic defects in mammalian models of PKAN Suh Young Jeong orcid.org/0000-0002-6376-7001 Department Molecular & Medical Genetics, Oregon Health Science University, Portland, OR, USA Search for more papers by this author Penelope Hogarth Neurology, Andrew Placzek Medicinal Chemistry Core, Allison M Gregory Rachel Fox orcid.org/0000-0001-6265-2256 Dolly Zhen Jeffrey Hamada Marianne...
Poly(ADP-ribose) polymerase 14 (PARP14) is a member of the PARP family enzymes that transfer ADP-ribose from NAD+ to nucleophilic amino acids on target proteins, process known as mono-ADP-ribosylation (MARylation). PARP14 involved in normal immune function through IL-4 signaling pathway and prosurvival factor multiple myeloma hepatocellular carcinoma. A mechanistic understanding physiological pathophysiological roles has been limited by dearth PARP14-specific MARylation targets. Herein we...
Identifying neurons that have functional opioid receptors is fundamental for the understanding of cellular, synaptic and systems actions opioids. Current techniques are limited to post hoc analyses fixed tissues. Here we developed a fluorescent probe, naltrexamine-acylimidazole (NAI), label based on chemical approach termed 'traceless affinity labeling'. In this approach, high antagonist naltrexamine used as guide molecule transferring reaction acylimidazole at receptor. This generates dye...
Translation regulation is a fundamental component of gene expression, allowing cells to respond rapidly variety stimuli in the absence new transcription. The lack methods for profiling nascent proteomes distinct cell populations heterogeneous tissues has precluded an understanding translational physiologically relevant contexts. Here, we describe chemical genetic method that involves orthogonal enzyme-mediated incorporation clickable puromycin analogue into polypeptides. Using this method,...
Accumulation of secondary metabolites is one the common reactions plants to ozone exposure in nature. To investigate effect on production desired compounds plant cell cultures, we assayed hypericin Hypericum perforatum suspension cultures treated with different doses at culture phases. The results show that contents cells 60 180 nL L(-1) are significantly higher than those control, showing may stimulate synthesis. Hypericin exponential phase lag and stationary phase, which suggests more...
The endochin-like quinolone (ELQ) compound class may yield effective, safe treatments for a range of important human and animal afflictions. However, to access the public health potential this series, synthetic route needed be devised, which would lower costs amenable large-scale production. In new described here, substituted β-keto ester, formed by an Ullmann reaction subsequent acylation, is reacted with aniline via Conrad–Limpach produce 3-substituted 4(1H)-quinolones such as ELQ-300...
Myocardial infarction (MI) triggers an inflammatory response that transitions from pro-inflammatory to reparative over time. Restoring sympathetic nerves in the heart after MI prevents arrhythmias. This study investigated if reinnervation altered immune MI. used quantitative multiplex immunohistochemistry identify cells present 2 weeks ischemia-reperfusion. Two therapeutics stimulated reinnervation, preventing arrhythmias and shifting reparative, with fewer macrophages more regulatory T...
PARP16-the sole ER-resident PARP family member-is gaining attention as a potential therapeutic target for cancer treatment. Nevertheless, the precise function of catalytic activity PARP16 is poorly understood. This primarily due to lack inhibitors that are selective over other members. Herein, we describe structure-guided strategy generating inhibitor by incorporating two selectivity determinants into phthalazinone pan-PARP scaffold: (i) an acrylamide-based (DB008) designed covalently react...
Elicitations are considered to be an important strategy improve production of secondary metabolites plant cell cultures. However, mechanisms responsible for the elicitor-induced cells have not yet been fully elucidated. Here, we report that treatment Catharanthus roseus suspension cultures with PB90, a protein elicitor from Phytophthora boehmeriae, induced rapid increases abscisic acid (ABA) and nitric oxide (NO), subsequently followed by enhancement catharanthine up-regulation Str Tdc, two...
Exposure to ozone induced a rapid increase in the levels of phytohormone abscisic acid (ABA) and sequentially followed by enhancement Taxol production suspension cell cultures Taxus chinensis. The observed increases ABA were dependent on concentration applied T. chinensis cultures. To examine role ozone-induced production, we pretreated cells with biosynthesis inhibitor fluridone abolish ozone-triggered generation assayed effect production. results showed that pretreatment not only...
The innate immune response to cytosolic DNA involves transcriptional activation of type I interferons (IFN-I) and proinflammatory cytokines. This represents the culmination intracellular signaling pathways that are initiated by pattern recognition receptors engage require adaptor protein Stimulator Interferon Genes (STING). These responses lead generation cellular tissue states impair microbial replication facilitate establishment long-lived, antigen-specific adaptive immunity. Ultimately...
Chondroitin sulfate proteoglycans (CSPGs) prevent sympathetic nerve regeneration in the heart after myocardial infarction and central regrowth traumatic brain injury spinal cord injury. Currently, there are no small-molecule therapeutics to promote through CSPG-containing scars. CSPGs bind monomers of receptor protein tyrosine phosphatase sigma (PTPσ) on surface neurons, enhancing ability PTPσ dephosphorylate tropomyosin kinases (Trks), inhibiting their activity preventing axon outgrowth....
MR1-restricted T (MR1T) cells recognize microbial small molecule metabolites presented on the MHC Class I-like MR1 and have been implicated in early effector responses to infection. As a result, there is considerable interest identifying chemical properties of metabolite ligands that permit recognition by MR1T cells, for consideration therapeutic or vaccine applications. Here, we made modifications known evaluate effect cell activation. Specifically, modified 6,7-dimethyl-8-D-ribityllumazine...
Abstract MR1-restricted T (MR1T) cells recognize microbial small molecule metabolites presented on the MHC Class I-like MR1 and have been implicated in early effector responses to infection. As a result, there is considerable interest identifying chemical properties of metabolite ligands that permit recognition by MR1T cells, for consideration therapeutic or vaccine applications. Here, we made modifications known evaluate effect cell activation. Specifically, modified...
Objective To study the expression and clinical significance of 14-3-3e Peroxiredoxin 1 in giant cell tumor bone.Methods The were examined 13 cases benign bone 11 aggressive by immunohisto chemical.Results In bone,the intense positive rate for 23.08％ 63.63％ (P =0.953).The 61.54％ 18.18％,respectively.There was significant difference rates =0.0472).Conclusion positively related to invasiveness prognosis bone.The combined detection 14-3-3eand may be valuable predicting guiding therapy patients...
We sought to develop a small-molecule activator of interferon regulatory factor 3 (IRF3), an essential innate immune transcription factor, which could potentially be used therapeutically in multiple disease settings. Using high-throughput screen, we identified entities that activate type I response, with minimal off-target NFκB activation. 399 compounds at hit rate 0.24% from singlicate primary screening. Secondary screening included the hits and additional similar chemical structures...
Abstract MR1-restricted T (MR1T) cells recognize microbial small molecule metabolites presented on the MHC Class I-like MR1 and have been implicated in early effector responses to infection. As a result, there is considerable interest identifying chemical properties of metabolite ligands that permit recognition by MR1T cells, for consideration therapeutic or vaccine applications. Here, we made modifications known evaluate effect cell activation. Specifically, modified...