A5101728590- Enzyme Production and Characterization
- Glycosylation and Glycoproteins Research
- Microbial Metabolites in Food Biotechnology
- Dysphagia Assessment and Management
- Voice and Speech Disorders
- Carbohydrate Chemistry and Synthesis
- Cancer, Hypoxia, and Metabolism
- Cancer Research and Treatments
- Phonetics and Phonology Research
- Photosynthetic Processes and Mechanisms
- Cancer Cells and Metastasis
- Plant Genetic and Mutation Studies
- Plant Stress Responses and Tolerance
- ATP Synthase and ATPases Research
- Mitochondrial Function and Pathology
- Click Chemistry and Applications
- Flavonoids in Medical Research
- Chemical Synthesis and Analysis
- Thyroid Cancer Diagnosis and Treatment
- Biofuel production and bioconversion
- Plant Molecular Biology Research
- Oral and Maxillofacial Pathology
- Soft tissue tumor case studies
- Histone Deacetylase Inhibitors Research
- Synthesis and Biological Activity
Zhejiang Hospital
2024
Shanghai Jiao Tong University
1994-2023
Shanghai Children's Hospital
2018-2023
Sichuan University
2011-2022
West China Hospital of Sichuan University
2011-2022
Zhongnan Hospital of Wuhan University
2022
Wuhan University
2022
Huaiyin Institute of Technology
2022
State Key Laboratory of Hybrid Rice
2020-2021
Shanghai First People's Hospital
2020-2021
SUMMARY Jasmonates (JAs) are key phytohormones that regulate plant responses and development. JASMONATE‐ZIM DOMAIN (JAZ) proteins safeguard JA signaling by repressing JA‐responsive gene expression in the absence of JA. However, interaction cooperative roles JAZ repressors remain unclear during Here, we found OsJAZ6 interacts with OsJAZ1 depending on a single amino acid so‐called ZIM domain rice transduction JA‐regulated spikelet In vivo protein distribution analysis revealed content is...
Accumulation of secondary metabolites is one the common reactions plants to ozone exposure in nature. To investigate effect on production desired compounds plant cell cultures, we assayed hypericin Hypericum perforatum suspension cultures treated with different doses at culture phases. The results show that contents cells 60 180 nL L(-1) are significantly higher than those control, showing may stimulate synthesis. Hypericin exponential phase lag and stationary phase, which suggests more...
Abstract Floral patterning is regulated by intricate networks of floral identity genes. The peculiar MADS32 subfamily genes, absent in eudicots but prevalent monocots, control organ identity. However, how the family genes interact with other homeotic during flower development mostly unknown. We show here that rice transcription factor OsMADS32 regulates interacting synergistically E class protein OsMADS6 a dosage-dependent manner. Furthermore, our results indicate important roles for...
Here, we describe an Se-auxiliary mediated ligation protocol capable of rapid native chemical ligations at sterically hindered junctions, followed by in situ auxiliary cleavage under neutral conditions without affecting unprotected Cys residues. This auxiliary, which is prepared from phenyl acetaldehyde one step, can be conveniently attached to the N-terminal region a peptide via reductive amination or coupling reaction. We demonstrated this methodology synthesizing two protein samples.
Here, we describe a convergent synthesis of the human granulocyte-macrophage colony-stimulating factor (hGM-CSF) harboring both O- and N-glycosylation sites using Se-auxiliary-mediated ligation protocol together with native chemical methodology. The robust rapid Se-mediated enables assembly N-terminus hGM-CSF in just 4 h, which is significantly faster than traditional ligation/desulfurization method. Therefore, this new methodology could help to produce glycoform libraries more efficiently...
Objectives Alcohol and its metabolites, such as acetaldehyde, induced hepatic mitochondrial dysfunction play a pathological role in the development of alcohol-related liver disease (ALD).
The aim of this study was to investigate differences and correlations between the pediatric Voice Handicap Index (pVHI) Voice-Related Quality-of-life (pVRQOL) questionnaires in children with without voice disorders. This a cross-sectional study.A total 418 parents aged 2 14 years participated from May 2016 July 2017. included 221 disorders (dysphonic group) 197 (control group). scores for pVHI pVRQOL were compared, correlation analysis performed.Compared control group, dysphonic group had...
Abstract Sirtuin 2 (SIRT2) is one of seven mammalian homologs silent information regulator (Sir2) and an NAD + ‐dependent deacetylase; however, its critical role in lymphangiogenesis remains to be explored. We investigate SIRT2 mediated regulation vascular endothelial growth factor D (VEGFD) expression by deacetylating PAS domain protein 1 (EPAS1) head neck cancer (HNC) vitro vivo. In this study, we report that SIRT2, rather than other members the Sir2 family, reduces VEGFD hypoxia‐induced...
Cancer is a leading cause of death worldwide. Recent research studies have revealed that GM3 derivatives considerable promise as potential therapeutic agents for cancer. To discover novel antitumor agents, one-pot enzymatic synthesis was established, yielding 14 in high total yields (22–41%). Subsequently, the inhibitory activities were assessed by wound-healing assays and Transwell tumor-bearing animal models. Among all derivatives, N-12 showed excellent migration invasion effects cells...
Single-port robot-assisted laparoscopic surgery (S-RALS) is rarely applied in pediatric surgery. There still no study on the application of S-RALS for resection benign ovarian tumors. The current aimed to investigate safety and feasibility tumors using da Vinci Xi system.The clinical data three patients who underwent Department Pediatric Surgery, Zhongnan Hospital Wuhan University from May 2020 September 2021 were retrospectively analyzed. mean age these children was 7.9 years (5.8-9.3...
The body volume constraint hypothesis is a widely accepted notion that explains proximate limitation of female reproductive output, but lacks empirical support. To examine how determines we experimentally manipulated the lacertid lizard (Takydromus septentrionalis), to whether reduction in caused output. Clutch size and mass decreased with volume. Reproductive output (residual clutch mass, R(CM)) was negatively related (BVR) (R(CM)=-0.008BVR+0.225). In contrast, neither egg nor offspring...
To find fractions from Spatholobi caulis with cytotoxic effects on cancer cell lines, screening tests were carried out using the SRB assay HL60 line. Further investigation and another four human lines (KB, K562, MCF-7 Hep G2), revealed a dose-dependent response. High-performance liquid chromatography coupled electrospray ionization-tandem mass spectrometry (HPLC- ESI-MSn) was used to isolate identify active constituents fraction. Three (F-IV, F-V F-VII) showed in vitro cytotoxicity....
Stress-associated proteins (SAP), a class of zinc-finger proteins, have been identified as novel stress regulatory in responses. However, SAP genes eggplant (SmSAP) little reported. It has important significance identifying members, understanding the molecular mechanisms underlying responses, and tolerance. We performed comprehensive study A20/AN1 domains, motifs, gene structures, phylogenetic relationships, chromosomal locations, replications, collinearity, cis-acting elements, expression...
Thyroid stimulating hormone deficiency is the cornerstone of treatment for metastatic thyroid cancer. Due to loss follicular epithelial cells in cancer, gland degenerates 85% its original size. When restored, cancer regenerate, which postulated be related stem-like cells. By single cell RNA seq, we found a group rare mouse expressed genes (CD44V6+ and CD133+) large number differentiated CD24+). In organoids, two subsets contribute equally regeneration. The analysis human revealed that...
Chemical investigation of the roots Viburnum dilatatum Thunb resulted in isolation and characterization a new phenolic glycosides 2-(-glucopyranosyloxy)-benzyl 3-(-glucopyranosyloxy)-benzoate (1), together with two known compounds. Their structures were established by spectroscopic means comparison literature values.
β-thalassemia is an inherited blood disease caused by reduced or inadequate β-globin synthesis due to gene mutation. Our previous study developed a gene-edited mice model (β654-ER mice) CRISPR/Cas9-mediated genome editing, targeting both the βIVS2-654 (C > T) mutation site and 3' splicing acceptor at 579 corrected abnormal mRNA in β654-thalassemia mice. Herein, we further explored therapeutic effect of hematopoietic stem cells (HSCs) from β654-ER on consecutive HSC transplantation. The...