A5013673801- Neuroscience and Neuropharmacology Research
- Axon Guidance and Neuronal Signaling
- Neural dynamics and brain function
- Stress Responses and Cortisol
- Neurogenesis and neuroplasticity mechanisms
- Neuroendocrine regulation and behavior
- Neuroscience and Neural Engineering
- Nerve injury and regeneration
- Neurotransmitter Receptor Influence on Behavior
- Receptor Mechanisms and Signaling
- Photoreceptor and optogenetics research
- Zebrafish Biomedical Research Applications
- Cell Image Analysis Techniques
- Neurological disorders and treatments
- Parkinson's Disease Mechanisms and Treatments
- Mitochondrial Function and Pathology
- Memory and Neural Mechanisms
- bioluminescence and chemiluminescence research
- Stroke Rehabilitation and Recovery
- Sleep and Wakefulness Research
- Advanced Fluorescence Microscopy Techniques
- Adipose Tissue and Metabolism
- Cellular transport and secretion
- Functional Brain Connectivity Studies
- Neurological Disorders and Treatments
Mie University
2022-2024
Yokohama City University
2010-2021
Kitasato University
2005-2006
Brain damage such as stroke is a devastating neurological condition that may severely compromise patient quality of life. No effective medication-mediated intervention to accelerate rehabilitation has been established. We found small compound, edonerpic maleate, facilitated experience-driven synaptic glutamate AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic-acid) receptor delivery and resulted in the acceleration motor function recovery after cortex cryoinjury mice training-dependent...
Significance Social separation early in life can lead to the development of impaired interpersonal relationships and profound social disorders. However, underlying cellular molecular mechanisms involved are largely unknown. In a rat model neonatal isolation, we examined dominance juveniles. We further investigated relationship between actin dynamics glutamate synaptic AMPA receptor delivery spines medial prefrontal cortex (mPFC) isolated animals. Here, report that isolation alters mPFC by...
Experience-dependent plasticity is limited in the adult brain, and its molecular cellular mechanisms are poorly understood. Removal of myelin-inhibiting signaling protein, Nogo receptor (NgR1), restores neural plasticity. Here we found that, NgR1-deficient mice, whisker experience-driven synaptic α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPAR) insertion barrel cortex, which normally complete by 2 weeks after birth, lasts into adulthood. In vivo live imaging two-photon...
The semaphorin family is a well-characterized of secreted or membrane-bound proteins that are involved in activity-independent neurodevelopmental processes, such as axon guidance, cell migration, and immune functions. Although semaphorins have recently been demonstrated to regulate activity-dependent synaptic scaling, their roles Hebbian plasticity well learning memory remain poorly understood. Here, using rodent model, we found an inhibitory avoidance task, hippocampus-dependent contextual...
Collapsin response mediator proteins (CRMPs) have been identified as mediating of repulsive axon guidance cue Semaphorin-3A (Sema3A). Phosphorylation CRMPs plays a crucial role in the Sema3A signaling cascade. It has shown that Fyn phosphorylates CRMP1 at Tyrosine 504 residue (Tyr504); however, physiological this phosphorylation not examined. We found was most strongly phosphorylated by among five members CRMPs. confirmed Tyr504 Fyn. Immunocytochemistry mouse dorsal root ganglion (DRG)...
In neonates, the stress of social isolation can alter developing neural circuits and cause mental illness. However, molecular cellular bases for these effects are poorly understood. Experience-driven synaptic AMPA receptor delivery is crucial circuit organisation during development. rat, whisker experience drives glutamate subunit 4 (GluA4) but not 1 (GluA1) to layer 4-2/3 pyramidal synapses in barrel cortex postnatal day (P)8-10, whereas GluA1 GluA4 delivered P12-14. We recently reported...
Deprivation of one modality can lead to the improvement other intact modalities. We have previously reported that visual deprivation drives AMPA receptors into synapses from layer4 2/3 in barrel cortex and sharpens functional whisker-barrel map at layer2/3 2 days after beginning deprivation. Enhanced excitatory synaptic transmission layer4-2/3 is transient returns base line level a week Here we found sharpened function maintained least for visually deprived animals. While increased...
Abstract Photosensitizing fluorescence protein is a promising tool for chromophore-assisted light inactivation (CALI) that enables specific oxidation and of intracellular molecules. However, commonly used monomeric photosensitizing fluorescent protein, SuperNova, shows low CALI efficiency due to its insufficient maturation at 37 °C, thereby limiting the application various molecules, especially in mammalian cells. Here, we present HyperNova, with markedly improved leading greatly enhanced...
In the authors' previous study, they observed that amino acids 157-171 of von Hippel-Lindau protein (VHL peptide) induced neuronal differentiation skin-derived precursors. They also noted transplantation these differentiated cells into striata a Parkinson disease (PD) rat model reduced apomorphine-induced rotations. present investigated if produce dopamine in striatum.Skin-derived precursors were neurons using VHL peptide and transplanted PD rats. Four weeks after transplantation, probe was...
Abstract Exposure to a stressful environment early in life can cause psychiatric disorders by disrupting circuit formation. Actin plays central roles regulating neuronal structure and protein trafficking. We have recently reported that neonatal isolation inactivated ADF/cofilin, the actin depolymerizing factor, resulted reduced dynamics at spines an attenuation of synaptic α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor delivery juvenile rat medial prefrontal cortex...
Functional recovery from brain damage, such as stroke, is a plastic process in the brain. The excitatory glutamate α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor (AMPAR) plays crucial role neuronal functions, and synaptic trafficking of AMPAR fundamental mechanism underlying plasticity. We recently identified collapsin response mediator protein 2 (CRMP2)-binding compound, edonerpic maleate, which augments rehabilitative training-dependent functional damage by facilitating...