A5091519731- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- Immune cells in cancer
- interferon and immune responses
- Immune Response and Inflammation
- Hematopoietic Stem Cell Transplantation
- NF-κB Signaling Pathways
- Toxoplasma gondii Research Studies
- Cancer, Stress, Anesthesia, and Immune Response
- Immunotherapy and Immune Responses
- Microbial infections and disease research
- Galectins and Cancer Biology
Klinikum rechts der Isar
2014-2018
Technical University of Munich
2013-2017
LMU Klinikum
2014
Abstract Maintaining immune tolerance requires the production of Foxp3-expressing regulatory T (Treg) cells in thymus. Activation NF-κB transcription factors is critically required for Treg cell development, partly via initiating Foxp3 expression. activation controlled by a negative feedback regulation through ubiquitin editing enzyme A20, which reduces proinflammatory signaling myeloid and B cells. In naive CD4+ cells, A20 prevents kinase RIPK3-dependent necroptosis. Using mice deficient...
Activation of the C-type lectin receptor Dectin-1 by β-glucans triggers multiple signals within DCs that result in activation innate immunity. While these mechanisms can potently prime CD8+ cytotoxic T-cell (CTL) responses without additional adjuvants, effector pathways control CTL induction remain unclear. Here we demonstrate Dectin-1-induced cross-priming mice does not require inflammasome but strictly depends on adapter protein Card9 vitro. In vivo, Dectin-1-mediated after vaccination...
Abstract The NF‐κB regulator A20 limits inflammation by providing negative feedback in myeloid cells and B cells. Functional lack of has been linked to several inflammatory autoimmune diseases. To define how affects the functionality T effector a highly environment, we performed conventional allogeneic hematopoietic stem cell transplantation (allo‐HSCT) with A20‐deficient CD4 + CD8 donor mice. Severity mortality graft‐versus‐host disease (GVHD) after allo‐HSCT was drastically reduced...
Mycoplasma are a frequent and occult contaminant of cell cultures, whereby these prokaryotic organisms can modify many aspects physiology, rendering experiments that conducted with such contaminated cells problematic. Chronic contamination in human monocytic lines has been associated suppressed Toll-like receptor (TLR) function. In contrast, we show here components derived from hyorhinis-infected line activate innate immunity non-infected primary immune cells. Release pro-inflammatory...
3081 Background: Strong inter-individual variation in clinical response to immune checkpoint inhibitors (ICB) including anti-CTLA-4 remains a major challenge, but the molecular pathways that modulate ICB efficacy remain ill defined. Methods: Using CRISPR/Cas9 technology generate melanoma cells lines lack nucleic acid receptors or downstream signaling molecules together with available genetically deficient mouse models, we addressed importance of receptor both tumor and host for...
Abstract Introduction: Targeting inhibitory T-cell receptors such as CTLA-4 has shown great promise in cancer therapy. However, strong inter-individual variation clinical response to checkpoint inhibitors remains a major challenge. Expression of viral defense genes human melanomas including the cytosolic RNA receptor RIG-I (DDx58) recently been associated with benefit blockade. Methods: Using CRISPR/Cas9 technology generate B16 melanoma cells lines that lack nucleic acid or downstream...