A5070454347- Ion channel regulation and function
- Ion Transport and Channel Regulation
- Ion Channels and Receptors
- Spectroscopy and Quantum Chemical Studies
- Neuroscience and Neuropharmacology Research
- Calcium signaling and nucleotide metabolism
- Photoreceptor and optogenetics research
- Neurobiology and Insect Physiology Research
- Mitochondrial Function and Pathology
- Sphingolipid Metabolism and Signaling
- ATP Synthase and ATPases Research
- Adenosine and Purinergic Signaling
- Lipid Membrane Structure and Behavior
- Neuroscience of respiration and sleep
University of Lausanne
2015-2021
University of Pavia
2015
Significance Many physiological processes are regulated by pH. The acid-sensing ion channels (ASICs) neuronal pH sensors involved in learning, fear behavior, neurodegeneration after ischemic stroke, and pain sensation. mechanism which acidic activates ASICs is still poorly understood. We show here that the “acidic pocket,” binding site of several toxins, not essential for channel function but has, rather, a modulatory role. Furthermore, we describe structural rearrangements occurring this...
Acid-sensing ion channels (ASICs) are neuronal Na+-permeable that activated by extracellular acidification and involved in fear sensing, learning, neurodegeneration after ischemia, pain sensation. We have recently found the human ASIC1a (hASIC1a) wild type (WT) clone which has been used many laboratories recombinant expression studies contains a point mutation occurs with very low frequency humans. Here, we compared function of WT this rare variant, highly conserved residue Gly212 is...
Acid-sensing ion channels (ASICs) act as pH sensors in neurons. ASICs contribute to pain sensation, learning, fear behavior and neuronal death after ischemic stroke. Extracellular acidification induces a transient activation subsequent desensitization of these Na+-selective channels. are trimeric made identical or homologous subunits. We have previously shown that mutation the highly conserved Gly212 residue human ASIC1a Asp affects channel function. is located proximity predicted Cl-...
Acid-sensing ion channels (ASICs) are neuronal Na + that activated by a drop in pH. Their established physiological and pathological roles, involving fear behaviors, learning, pain sensation, neurodegeneration after stroke, make them promising targets for future drugs. Currently, the ASIC activation mechanism is not understood. Here, we used voltage-clamp fluorometry (VCF) combined with fluorophore-quencher pairing to determine kinetics direction of movements. We show conformational changes...
Abstract Acid-sensing ion channels (ASICs) are neuronal Na + that activated by a drop in pH. Their established physiological and pathological roles, involving fear behaviors, learning, pain sensation neurodegeneration after stroke, make them promising targets for future drugs. Currently, the ASIC activation mechanism is not understood. Here we used voltage-clamp fluorometry (VCF) combined with fluorophore-quencher pairing to determine kinetics direction of movements. Molecular dynamics...