A5030372272- Immune Cell Function and Interaction
- Inflammasome and immune disorders
- Allergic Rhinitis and Sensitization
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Food Allergy and Anaphylaxis Research
- Dermatology and Skin Diseases
- Plant Reproductive Biology
- T-cell and B-cell Immunology
- IL-33, ST2, and ILC Pathways
- Toxin Mechanisms and Immunotoxins
- Monoclonal and Polyclonal Antibodies Research
- Fungal Infections and Studies
- Microbial Inactivation Methods
- Escherichia coli research studies
- CAR-T cell therapy research
- Autoimmune and Inflammatory Disorders Research
- Urticaria and Related Conditions
- Antimicrobial Peptides and Activities
- Bee Products Chemical Analysis
- HIV Research and Treatment
- Nail Diseases and Treatments
- Salmonella and Campylobacter epidemiology
- Contact Dermatitis and Allergies
- Systemic Lupus Erythematosus Research
University of Tokyo Hospital
2021
Hayashibara (Japan)
1989-2004
University of Helsinki
2000
Institute for Molecular Medicine Finland
2000
Heidelberg University
1999
The University of Tokyo
1999
Johannes Gutenberg University Mainz
1997
Okayama University
1988-1997
University of the Ryukyus
1997
Hyogo Medical University
1997
Abstract We have recently reported that a novel molecule, murine IFN-gamma-inducing factor (IGIF) produced by mouse liver cells, possesses potent biologic activities, including the induction of IFN-gamma production spleen cells and enhancement NK cell cytotoxicity. In this paper, we report on isolation human IGIF cDNA clones from normal libraries using as probe. The amino acid sequence deduced indicated 193-amino precursor peptide revealed 65% homology with IGIF. also included an IL-1...
We have previously reported the cloning of a novel cytokine, IFN-gamma-inducing factor (IGIF), which shared some biologic activities with IL-12. In this study, we analyzed effects murine IGIF on activation T cells, and compared those alone had no effect cell lines or Th1 clones, while increased IFN-gamma production by antigen-stimulated lines, but IL-4 IL-10 production. As IL-12, served as costimulatory for clones stimulated Ag B APC, immobilized anti-CD3, Con A, IL-2 to augment induce IL-2R...
IL-12 and IFN-gamma-inducing factor (IGIF) have the capacity to stimulate IFN-gamma production by T cells. Using an IL-12-responsive cell clone, 2D6, we investigated how these two cytokines collaborate for production. 2D6 obtained from cultures containing rIL-12 produced in response rIGIF. deprived of 24 h only marginal levels following stimulation with either or rIGIF alone. However, simultaneous cells both resulted strikingly enhanced could also be maintained presence rIL-2 instead rIL-12....
Recently, the novel cytokine IL-18 (IFN-gamma-inducing factor) has been described as a growth and differentiation factor for Th1 cells. Epidermal keratinocytes (KC) are known to direct T cell education by production of cytokines. Therefore, expression was sought in KC. RNA analyzed following stimulation with contact sensitizers or controls mRNA semiquantitative reverse transcription-PCR. Constitutive low untreated epidermal cells (EC), but early up-regulation signals detected application...
We examined the ability of interleukin-12 (IL-12) and IL-18 to induce production gamma interferon (IFN-gamma) nitric oxide (NO) by murine peritoneal exudate cells (PEC) stimulate growth-inhibitory activity these against Cryptococcus neoformans. PEC produced IFN-gamma NO when stimulated with a combination IL-12 but little or no either cytokine was used alone. anticryptococcal mediated production, since it completely inhibited neutralizing anti-IFN-gamma monoclonal antibody (MAb)...
IL-18 inhibits osteoclast (OCL) formation in vitro independent of IFN-gamma production, and this was abolished by the addition neutralizing antibodies to GM-CSF. We now establish that unable inhibit OCL cocultures using GM-CSF-deficient mice (GM-CSF -/-). Reciprocal either wild-type osteoblasts with GM-CSF -/- spleen cells or were examined. Wild-type required elicit a response indicating splenic origin target. As T comprise large proportion cell population, role osteoclastogenesis Total...
ABSTRACT Spleen and peritoneal macrophages obtained from innately resistant A/J mice released low levels of interleukin 18 (IL-18) upon infection with Salmonella typhimurium C5 RP4. Incubating the cells recombinant gamma interferon (rIFN-γ) enhanced IL-18 production. treated in vivo anti-IL-18 antibodies showed impaired resistance to infection, increased bacterial loads liver spleen. Administration rIL-18 could protect challenge a lethal dose virulent salmonellae, dramatic reduction numbers...
We examined the effects of a newly identified cytokine, IL-18, originally designated as IFN-gamma-inducing factor, in mouse model pulmonary and disseminated infection with highly virulent strain Cryptococcus neoformans. Administration murine rIL-18 enhanced elimination live micro-organisms from lungs, prevented fungal dissemination to brain, reduced level serum cryptococcal capsular polysaccharide Ags, increased survival rate infected mice. Histologic examination lung sections PBS-treated...
Interleukin (IL)-18 is an interferon (IFN)-gamma-inducing cytokine suggested to be important in regulating inflammatory responses. This study investigated the pulmonary expression of IL-18 under conditions characterized by T-helper (Th)1 (lipopolysaccharide (LPS) treatment/sarcoidosis) and Th2 (ovalbumin (OVA) challenge/asthma) production. In situ hybridization immunocytochemistry were used determine number cells expressing IL-18, IFN-gamma, IL-5 major basic protein (MBP) within lung tissue...
In the course of analyzing partial amino acid sequences Cry j I, a major allergen Japanese cedar (Cryptomeria japonica) pollen, we found peptide fragment which has significant homology to some pectate lyase isozymes secreted by plant pathogenic bacteria. Therefore, investigated whether I activity. reacted with polygalacturonic acid, resulting in release unsaturated uronide products. The optimum temperature and pH for reaction were 60–70°C 10. enzymatic had an absolute Ca 2+ ion requirement....
Japanese cedar (Cryptmeria japonica: CJ) pollinosis is one of the most important allergic diseases in Japan. Recently, second major allergen (Cry j II) was isolated from CJ pollen. There have been no prevalence studies sensitivity to Cry I and II among a large number patients with pollinosis.This study conducted evaluate II. We measured specific IgE antibodies these allergens sera 145 patients. Furthermore, comparison examined by histamine release assay.Specific were assayed fluorometric...
Genetic lack of interleukin 12 receptor β1 (IL-12Rβ1) surface expression predisposes to severe infections by poorly pathogenic mycobacteria or Salmonella and causes strongly decreased, but not completely abrogated, interferon (IFN)-γ production. To study IL-12Rβ1–independent residual IFN-γ production, we have generated mycobacterium–specific T cell clones (TCCs) from IL-12Rβ1–deficient individuals. All TCCs displayed a helper type 1 phenotype the majority responded IL-12 increased production...
To examine the intrathecal production of a newly identified cytokine, interferon-gamma-inducing factor (IL-18), together with interferon-gamma itself, in inflammatory diseases CNS (i.e., bacterial meningitis, viral meningoencephalitis, and MS).IL-18 concentrations CSF were significantly increased meningitis tended toward levels meningoencephalitis. In contrast, IL-18 was detectable only few patients MS healthy controls. Interestingly, selectively meningoencephalitis.The observation an...
SUMMARY Intestinal epithelial cell (IEC)-derived cytokines, such as stem factor (SCF), interleukin (IL)-7 and IL-15 are known to be required for the development of intestinal intraepithelial lymphocytes (IELs). A newly described cytokine, IL-18, has also been shown produced by cells. To demonstrate functional effects IL-18 on human IELs, we assessed IL-18/IL-18 receptor expression in IEC/IEL proliferation following stimulation IELs IL-18. transcripts were detected both freshly isolated...
SUMMARY Primary biliary cirrhosis is an autoimmune disease of the liver in which T helper 1 cytokines predominate over those 2 pathogenesis. Interleukin-18 (IL-18), for gene was recently cloned, a novel cytokine, augments interferon-gamma production. We designed this study to clarify role IL-18 primary and examine whether serum level can be prognostic indicator disease. Serum levels were measured using enzyme linked immuno sorbent assay with mouse monoclonal antibodies. Twenty-two healthy...
We examined Cry j 2, a major allergen of Japanese cedar (Cryptomeria japonica) pollen, for polygalacturonase enzyme activity, since nucleotide sequence cDNA 2 showed significant homology with that tomato polygalacturonase. Polygalacturonase is well known to depolymerize preferentially polygalacturonic acid (PGA) by hydrolysis. However, did not act on PGA, but was found pectin and methylesterified PGA in dose-dependent manner. The substrate specificity different from derived Aspergillus...
Abstract It is generally accepted that immunologically naive T cells display a very restricted cytokine production profile consisting mainly of IL-2, which used as an autocrine growth factor. Here we report activated CD4+ cells, neonatal or adult origin, express high levels soluble lymphotoxin (LT) α (LTα3), determined by ELISA, RNase protection assay, and intracytoplasmic staining. Besides LTα3 these also produce TNF-α together with significant amounts IFN-γ IL-13. Naive LTβ mRNA the...