The Phosphate Transporter1 (PHT1) family of genes plays pivotal roles in the uptake inorganic phosphate from soils. However, there is no comprehensive report on PHT1 Zea mays based whole genome. In present study, a total 13 putative (ZmPHT1;1 to 13) were identified inbred line B73 genome by bioinformatics methods. Then, their function was investigated yeast PHO84 mutant complementary experiment and qRT-PCR. Thirteen ZmPHT1 distributed six chromosomes (1, 2, 5, 7, 8 10) divided into two...

Photothermal therapy (PTT) as a kind of noninvasive tumor treatment has attracted increasing research interest. However, the efficiency existing PTT agents in near-infrared (NIR) region is major problem that hindered further development PTT. Herein, we present an effective strategy to construct efficient photothermal agent by utilizing intramolecular photoinduced electron transfer (PeT) mechanism, which able dramatically improve conversion NIR region. Specifically, dye (A1) constructed with...

Abstract It is always a huge challenge to develop novel near‐infrared (NIR) phototherapeutic agents suitable for imaging‐guided cancer therapy. In order clarify the positive heavy atom effects on photodynamic and photothermal efficiencies of agents, series chlorine‐, bromide‐, or iodine‐substituted aza‐BODIPYs (B2, B3, B4, respectively) are designed synthesized. Among them, B4 exhibits both excellent (singlet oxygen yield 1.57 times more than that B3) (1.3 °C higher B3). Then, nanoparticles...

Phototherapy, as an important class of noninvasive tumor treatment methods, has attracted extensive research interest. Although a large amount the near-infrared (NIR) phototherapeutic agents have been reported, low efficiency, complicated structures, tedious synthetic procedures, and poor photostability limit their practical applications. To solve these problems, herein, donor–acceptor–donor (D–A–D) type organic agent (B-3) based on NIR aza-boron-dipyrromethene (aza-BODIPY) dye constructed,...

Phosphorus (P) is an essential macronutrient for plant life, although it frequently not readily available to crops. Arbuscular mycorrhiza fungi (AMF) can improve P levels by inducing the expression of some phosphate (Pi) transporters. Symbiotic Pi uptake transporters crucial AMF colonization and arbuscule dynamics. However, functions mycorrhiza-inducible maize are largely unclear. We focused on interaction between concentration in maize, detecting induction a transporter. investigated...

Abstract Arbuscular mycorrhizal (AM) fungi traditionally form symbioses with most plant species. Although AM have critical effects on microbial communities, the pathways showing how shape rhizosphere bacterial communities and their functions are rarely explored. Here, through three systematic experiments, fungi–bacteria interactions were first investigated in of Lotus japonicus , then confirmed by a second experiment wild‐type mycorrhiza‐defective mutant ljcbx L. . The mechanisms presented...

Abstract Symbiosis with arbuscular mycorrhizal (AM) fungi improves plant nutrient capture from the soil, yet there is limited knowledge about diversity, structure, functioning, and assembly processes of AM fungi‐related microbial communities. Here, 16S rRNA gene sequencing metagenomic were used to detect bacteria in rhizosphere Lotus japonicus inoculated without fungi, L. mutant ljcbx (defective symbiosis) southern grassland soil. Our results show that symbiosis significantly increased...

Nucleotide-binding site-leucine-rich repeat (NBS-LRR) domain proteins are immune sensors and play critical roles in plant disease resistance. In this study, we cloned characterized a novel NBS-LRR gene ZmNBS25 maize. We found that could response to pathogen inoculation salicylic acid (SA) treatment maize, transient overexpression of induced hypersensitive tobacco. High-performance liquid chromatography (HPLC) analysis showed that, compared control plants, (ZmNBS25-OE) Arabidopsis rice...

The aim to develop a highly stable near-infrared (NIR) photoinduced tumor therapy agent stems from its considerable potential for biological application. Due long wavelength, imaging exhibits high signal-to-background ratio, deep tissue penetration and maximum permissible light power, which can minimize damage an organism during therapy.A class of NIR-II fluorophores (NIR998, NIR1028, NIR980, NIR1030, NIR1028-S) based on aza-boron-dipyrromethene (aza-BODIPY) dyes with donor-acceptor-donor...

Rational design of tumor-microenvironment (TME)-activated nanoformulation for precisely targeted cancer treatment has recently attracted an enormous attention. However, the all-in-one TME-activated theranostic nanosystems with a simple preparation and high biocompatibility are still rarely reported. Herein, catalase nanocrystals (CatCry) first introduced as tumor microenvironment activatable nanoplatform selective theranostics colon cancer. They engaged (i) "nanoreactor" silver nanoparticles...

The productivity of maize (Zea mays L.) depends on the development chloroplasts, and G2-like transcription factors play a central role in regulating chloroplast development. In this study, we identified 59 genes B73 genome systematically analyzed these at molecular evolutionary levels. Based gene structure character, motif compositions phylogenetic analysis, (ZmG1- ZmG59) were divided into seven groups (I-VII). By synteny 18 collinear pairs strongly conserved microsyntny among regions...

Abstract Recent studies indicate that the synergistic phototherapy (SPT) process can simultaneously generate heat for photothermal therapy (PTT) and singlet oxygen ( 1 O 2 ) photodynamic (PDT) to overcome recurrence of tumors. However, hypoxic environment in tumors seriously limits therapeutic effect oxygen‐dependent PDT, leading domination PTT SPT process. Therefore, it is urgent develop a novel platform overcoming hypoxia improving both PDT. In this work, phototherapeutic based on...

Arbuscular mycorrhizal fungi (AMF) can symbiose with many plants and improve nutrient uptake for their host plant. Rhizosphere microorganisms have been pointed to play important roles in helping AMF mobilize soil insoluble nutrients, especially phosphorus. Whether the change phosphate transport under colonization will affect rhizosphere is still unknown. Here, we evaluated links of interactions among bacterial community maize (Zea mays L.) by using a defective mutant. Loss symbiosis function...

Recently, long noncoding RNAs (lncRNAs) have emerged as vital regulators of many biological processes in animals and plants. However, to our knowledge no investigations on plant lncRNAs which respond arbuscular mycorrhizal (AM) fungi been reported thus far. In this study, maize roots colonized with AM fungus were analyzed by strand-specific RNA-Seq identify fungi-responsive construct an associated regulatory network. A total 1837 differentially expressed protein coding genes (DEGs)...

Maize can form symbiotic relationships with arbuscular mycorrhiza (AM) fungus to increase productivity and resistance, but the miRNAs in maize responsible for this process have not been discovered. In study, 155 known 28 novel were identified by performing high-throughput sequencing of sRNA roots colonized AM fungi. Similar profiles other AM-capable plants, a large proportion 24 nt length. Fourteen two significantly down- up-regulated response Glomus intraradices inoculation, respectively,...

14-3-3 proteins (regulatory protein family) are phosphate serine-binding proteins. A number of transcription factors and signaling have been shown to bind the in plants, which plays a role regulating their growth (seed dormancy, cell elongation division, vegetative reproduction stress response (salt stress, drought cold stress). Therefore, genes crucial controlling how plants respond develop. However, little is known about function gene families gramineae. In this study, 49 were identified...

The applications of conjugated polyelectrolytes in biosensing and bioimaging have attracted more research interests due to their excellent photophysical properties. In this work, a new series containing long‐lived phosphorescent Ir(III) complexes is designed synthesized, which can be used for ratiometric lifetime‐based sensing heparin utilizing the electrostatic interaction between cationic polymers anionic heparin. By changing ligand structures complexes, performances...

Insufficient reactive oxygen species originating from hypoxia and high glutathione (GSH) in the tumor microenvironment (TME) is an important reason for radiotherapy (RT) resistance. Currently, radiosensitizers that remodel TME are widely investigated to enhance RT. However, developing effective nano‐radiosensitization system removes radiotherapy‐resistant factors boost RT effect while visualizing imaging aid therapy remains a challenge. Herein, MnO 2 nanosheets grown on surface of ultrasmall...

Arbuscular mycorrhizal (AM) fungi can enhance plant uptake of phosphorus (P) and potassium (K), but it is not yet clear whether rhizosphere bacteria the ability AM to acquire insoluble P K from soil. Here, pot experiments confirmed that fungus-promoted by plants requires bacteria. The changes bacterial communities associated with were explored 16S rRNA amplicon sequencing metagenomic sequencing. Five core genera identified involved in cycles. Synthetic community (SynCom) inoculation revealed...

The development of innovative synergistic phototherapy (SPT) agent with excellent photodynamic therapy (PDT) and photothermal (PTT) effects for efficient antitumor is great significance. However, the low conversion efficiency or singlet oxygen yield SPT during process will result in inefficient tumor therapy. Herein, a new near‐infrared (NIR) based on aza‐platinum‐dipyrromethene dye ( D‐3 ) PDT PTT rationally designed synthesized by metallization halogenation aza‐dipyrromethene...