A5079202118- Ion Channels and Receptors
- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- Helicobacter pylori-related gastroenterology studies
- Heart Rate Variability and Autonomic Control
- Mesenchymal stem cell research
- Magnesium in Health and Disease
- ECG Monitoring and Analysis
- Ultrasound and Hyperthermia Applications
- Electrospun Nanofibers in Biomedical Applications
- Neuroscience and Neural Engineering
- Galectins and Cancer Biology
- Cardiovascular Function and Risk Factors
- Transplantation: Methods and Outcomes
- Probiotics and Fermented Foods
- Gut microbiota and health
- Cardiovascular and exercise physiology
- Neurobiology and Insect Physiology Research
- Tissue Engineering and Regenerative Medicine
- Cardiac Health and Mental Health
- Biochemical Analysis and Sensing Techniques
- Cardiovascular Effects of Exercise
- Trace Elements in Health
- Plant Micronutrient Interactions and Effects
- Recommender Systems and Techniques
Fukuoka University
2013-2025
Monash University
2024
Zhongda Hospital Southeast University
2008-2017
National Institutes of Natural Sciences
2016
Okazaki Institute for Integrative Bioscience
2016
National Institute for Physiological Sciences
2016
Sumitomo Dainippon Pharma (Japan)
2016
Fujita Health University
2016
Kyoto University
2016
Keck Graduate Institute
2008
Chronic inflammation is a risk factor for colorectal cancer, and inflammatory cytokines secreted from cells active oxygen facilitate tumorigenesis. Intestinal bacteria are thought to regulate The longer the breastfeeding period, lower of bowel disease. Here, we investigated preventive effects probiotic Lactobacillus rhamnosus M9 (Probio-M9) on colitis-associated An tumor model was established using 6-week-old male C57BL/6NCrSlc mouse, which intraperitoneally administered with azoxymethane...
Background & AimsThe transient receptor potential ankyrin 1 (TRPA1) channel is highly expressed in the intestinal lamina propria, but its contribution to gut physiology/pathophysiology unclear. Here, we evaluated function of myofibroblast TRPA1 channels remodeling.MethodsAn cell line (InMyoFibs) was stimulated by transforming growth factor-β1 induce vitro fibrosis. Trpa1 knockout mice were generated using Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9...
Introduction Connexins are gap junction proteins that play pivotal roles in intercellular communication. Connexin 43 (Cx43) is one of the most ubiquitously expressed connexin isoforms human. Cx43 has been demonstrated to be involved pathological process various diseases, including arrhythmias. Recently, translationally controlled tumor protein (TCTP), a highly conserved anti-apoptotic protein, shown an important role protecting against development heart failure. However, its arrhythmogenesis...
Intestinal fibrosis is a frequent complication of Crohn's disease (CD) and often leads to detrimental stricture formation. Myofibroblasts play active roles in mediating fibrotic changes various tissues. We investigated whether transient receptor potential channels intestinal myofibroblasts are involved CD-associated fibrosis.An myofibroblast cell line (InMyoFibs) was stimulated with transforming growth factor-β1 (TGF-β1) model excessive fibrosis. Biopsy samples from nonstenotic or stenotic...
To investigate the anti-fibrotic effects of traditional oriental herbal medicine Daikenchuto (DKT) associated with transient receptor potential ankyrin 1 (TRPA1) channels in intestinal myofibroblasts.Inflammatory and fibrotic changes were detected a 2,4,6-trinitrobenzenesulfonic acid (TNBS) chronic colitis model wild-type TRPA1-knockout (TRPA1-KO) mice via pathological staining immunoblotting analysis. Ca2+ imaging experiments examined DKT its components/ingredients on myofibroblast...
The molecular mechanism underlying Ca(2+)/calmodulin (CaM)-dependent kinase II (CaMKII)-mediated regulation of the mouse transient receptor potential channel TRPC6 was explored by chimera, deletion and site-directed mutagenesis approaches. Induction currents (ICCh) in TRPC6-expressing HEK293 cells a muscarinic agonist carbachol (CCh; 100 μm) strongly attenuated CaMKII-specific peptide, autocamtide-2-related inhibitory peptide (AIP; 10 μm). TRPC6/C7 chimera experiments showed that C-terminal...
Transient receptor potential canonical (TRPC) proteins form Ca<sup>2+</sup>-permeable cation channels activated upon stimulation of metabotropic receptors coupled to phospholipase C. Among the TRPC subfamily, TRPC3 and TRPC6 directly by diacylglycerol (DAG) play important roles in brain-derived neurotrophic factor (BDNF) signaling, promoting neuronal development survival. In various disease models, BDNF restores neurologic deficits, but its therapeutic is limited poor pharmacokinetic...
Mesenchymal stem cells (MSCs) transplantation provides a new approach for myocardial repair. However, many important fundamental questions about MSCs remain unanswered. There is an urgent need to identify from the beating heart and analyze efficacy of this approach. This study aimed localize magnetically labeled (MR-MSCs) monitor restorative effects MR-MSCs with magnetic resonance (MR) imaging.Acute infarction (AMI) was created in swine by balloon occlusion left anterior descending coronary...
Continuous Ca2+ influx is essential to maintain intracellular homeostasis and its dysregulation leads a variety of cellular dysfunctions. In this study, we explored the functional roles spontaneous for proliferation differentiation human erythromyeloid leukemia cell line K562. mRNA/protein expressions were assessed by real-time RT-PCR, western blotting, immunocytochemical staining. Intracellular concentration ([Ca2+ ]i ) ionic currents measured fluorescent imaging patch clamping techniques,...
Transient receptor potential cation channel subfamily melastatin member 4 (TRPM4), a Ca2+-activated nonselective abundantly expressed in the heart, has been implicated conduction block and other arrhythmic propensities associated with cardiac remodelling injury. The present study aimed to quantitatively evaluate arrhythmogenic of TRPM4.Patch clamp biochemical analyses were performed using expression system an immortalized atrial cardiomyocyte cell line (HL-1), numerical model simulation was...
A Ca2+-activated monovalent cation-selective TRPM4 channel is abundantly expressed in the heart. Recently, a single gain-of-function mutation identified distal N-terminus of human (Glu5 to Lys5; E7K) was found be arrhythmogenic because enhanced cell membrane expression. In this study, we conducted detailed analyses mutant from more functional aspects, comparison with its wild type (WT). an expression system, intracellular application short soluble PIP2 (diC8PIP2) restored single-channel...
In the heart, TRPM4 is most abundantly distributed in conduction system. Previously, a single mutation, ‘E7K’, was identified its distal N-terminus to cause disorder because of enhanced cell-surface expression. It remains, however, unclear how this expression increase leads failure rather than abnormally cardiac excitability. To address issue theoretically, we mathematically formulated gating kinetics E7K-mutant channel by combined use voltage jump analysis and ionomycin-perforated...
This research concentrates on the integration of Low-Rank Adaptation for Text-to-Image Diffusion Fine-tuning and Conditional Image Generation in e-commerce recommendation systems. Fine-tuning, skilled producing precise diverse images from aesthetic descriptions provided by users, is extremely valuable personalizing product suggestions. The enhancement interpretation textual prompts consequent image generation accomplished through fine-tuning cross-attention layers Stable model. In an effort...
Intestinal bacteria play important roles in the progression of colitis-associated carcinogenesis. Colostrum-derived Lacticaseibacillus rhamnosus Probio-M9 (Probio-M9) has shown a protective effect cancer (CAC) model, but detailed metagenomic analysis had not been performed. Here, we investigated preventive effects probiotic on CAC-model mice, tracking microbiota. Feces were obtained at four time points for evaluation gut The tight junction protein expression was evaluated co-cultured Caco-2...
Modeling and simulation of cardiac electrophysiology has the advantages economic security than anatomical experiments. However, a model that matches real one always demands very high computing ability an ordinary CPU could hardly satisfy. In this paper, parallelized solution 2D based on CUDA (Compute Unified Device Architecture) using GPUs is introduced. parallel platform API (Application Programming Interface) created released by NVIDIA, which offers us way as co-processors to implement...