Colistin is an antibiotic used as a last resort to treat multidrug-resistant Gram-negative bacterial infections. Plasmid-mediated mobile colistin-resistant (mcr) genes in Escherichia coli (E. coli) are disseminated globally and considered be major public health threat. This study aimed determine the molecular characteristics of isolates clinical settings Pakistan. A total 240 E. strains isolated from urine pus cultures were collected two hospitals Faisalabad analyzed for phenotypic...

Multiple myeloma (MM) is a hematologic cancer characterized by expansion of malignant plasma cells in the bone marrow. Most patients develop an osteolytic disease, largely caused increased osteoclastogenesis. The marrow hypoxic, and hypoxia may contribute to MM disease progression, including loss. Here we identified interleukin-32 (IL-32) as novel inflammatory cytokine expressed subset primary cell lines. We found that high IL-32 gene expression correlated with inferior survival was higher...

Nano-fertilizer(s), an emerging field of agriculture, is alternate option for enhancement plant growth replacing the synthetic fertilizers. Zinc oxide nanoparticles (ZnO NPs) can be used as zinc source plants. The present investigation was carried out to assess role ZnO NPs in promotion maize Biosynthesized (using Bacillus sp) were characterized using Scanning Electron Microscope (SEM), Transmission (TEM), X-ray diffraction (XRD) and Zeta potential. Different concentrations (2, 4, 8, 16...

In the current study; insecticidal, growth regulation, oviposition deterrence and repellency of petroleum ether extracts Azadirachta indica, Penganum harmala, Datura stramonium, Tribulus terrestris Chenopodium murale against 2nd instar larvae housefly was investigated. Five different concentrations (5%, 10%, 15%, 20% 25%) were used through larval feeding mortality data recorded after 24, 48 72 hrs. Highest induced by P. harmala (63.87%) followed D. stramonium (62.78%), A. indica (53.84%), T....

Background Cutaneous wound healing is a complex process involving several signaling pathways such as the Wnt and extracellular signal-regulated kinase (ERK) pathways. Valproic acid (VPA) commonly used antiepileptic drug that acts on these pathways; however, effect of VPA cutaneous unknown. Methods Findings We created full-thickness wounds backs C3H mice then applied VPA. After 7 d, we observed marked reduced size in VPA-treated mice. In neo-epidermis wounds, β-catenin markers for...

Pseudomonas aeruginosa is considered as foremost cause of hospital acquired infections due to its innate and plasmid mediated resistance multiple antibiotics making it a multi drug resistant (MDR) pathogen. Biofilm formation pathogenic mechanism harbored by this pathogen which further elevates host defense system. The aim the present study was evaluate biofilm forming potential distribution pslA gene in isolates obtained from different clinical samples. A total 200 samples were collected...

In developing countries, various sociodemographic and climatic factors such as urbanization, industrialization, improved living standard, water energy demands will all characterize wastewater’s future. Increasing population shortage are the main driving forces for reuse of untreated wastewater irrigation other uses in many poor posing a significant threat to global food security. Although contains essential nutrients required plant growth, it also toxic heavy metals pathogens that pose...

Background: Human brucellosis is a neglected and zoonotic disease also known as Malta fever or undulant fever. Objectives: A cross sectional study was conducted to determine the seroprevalence of access role risk factors associated with this in humans Punjab, Pakistan. Methods: total 250 serum samples were collected subjected Rose Bengal Plate Test Enzyme Linked Immunosorbent Assay for screening Brucella. predesigned questionnaire filled prior sampling collect data regarding socio -...

The Wnt/β-catenin pathway is a potential target for development of anabolic agents to treat osteoporosis because its role in osteoblast differentiation and bone formation. However, there no clinically available anti-osteoporosis drug that targets this pathway. In study, we screened library aqueous extracts 350 plants identified Hovenia dulcis Thunb (HDT) extract as activator. HDT induced osteogenic calvarial osteoblasts without cytotoxicity. addition, increased femoral mass inducing...

Drosophila melanogaster being used as model organism is considered pest of homes, restaurants, and fruit markets. The damaged fruits are also reported to serve a carrier for various diseases. current study was designed evaluate the toxicity petroleum extract some weed plants, namely, Euphorbia prostrata, Parthenium hysterophorus, Fumaria indica, Chenopodium murale, Azadirachta against D. melanogaster. Mortality at 10, 20, 30% concentrations after 24 48 hours found comparatively low. E....

Abstract Five different weed plants viz. Convulvulus arvensis, Chenopodium murale, Tribulus terrestris, Trianthema portulacastrum , and Achyranthes aspera were investigated for their entomocidal genotoxic effects against Culex quinquefasciatus mosquitoes. High mortality was observed at 72 hours in a dose dependent manner. Among all the tested plants, A. found highly significant which showed 100% 250 ppm after with LC 50 of 87.46, 39.08 9.22 24, 48, respectively. In combination Bacillus...

The prevalence of Giardia lamblia in Pakistani children is currently unknown. aim here was to evaluate the and risk factors exhibiting diarrhea.Cross-sectional study at different district healthcare hospitals Pakistan.A total 800 samples were collected from aged 0-10 years. Information regarding personal data, demographic data supposed through a structured questionnaire. detected direct microscopy antigens enzyme-linked immunosorbent assay (ELISA).The 2.75% inflated 9.5% ELISA. positively...

Urbanization and industrialization are the main anthropogenic activities that adding toxic heavy metals to environment. Among these, chromium (in hexavalent: Cr+6 and/or trivalent Cr+3) is being released abundantly in wastewater due its uses different industrial processes. It becomes highly mutagenic carcinogenic once it enters cell through sulfate uptake pathways after interacting with cellular proteins nucleic acids. However, can be bio-converted into more stable, less insoluble using...

Four commercial strains of chickens, namely, ISA brown leghorn (ISA), TETRA-SL (TETRA-SL), Lohmann (LB), and LSL (LSL), were infected with a well-defined clonal culture Histomonas meleagridis (H. meleagridis/Turkey/Austria/2922-C6/04) to investigate their susceptibility histomonosis. Each group included 16 which housed under the same conditions in separate pens. All chickens 104 histomonads orally intracloacally at 14 days age. No mortality or clinical signs observed during experiment all...

ABSTRACT Osteoporosis is a major global health issue in elderly people. Because Wnt/β-catenin signaling plays key role bone homeostasis, we screened activators of this pathway through cell-based screening, and investigated indirubin-3′-oxime (I3O), one the positive compounds known to inhibit GSK3β, as potential anti-osteoporotic agent. Here, show that I3O activated via inhibition interaction GSK3β with β-catenin, induced osteoblast differentiation vitro increased calvarial thickness ex vivo....

The emergence of resistance to beta-lactam agents in poultry results multidrug-resistant (MDR) phenotypes Escherichia coli isolates from birds. appearance mobile colistin (mcr) genes the sector has further worsened situation. Therefore, current study is aimed at investigating molecular epidemiology mcr harboring colistin-resistant E. among poultry.The isolation and identification (CR-Ec) were done broiler's fecal samples through culturing using selective media supplemented with sulfate (4...

In the present work, chickens and turkeys were infected with virulent or attenuated Histomonas meleagridis to investigate compare effect of both isolates on birds. Thereby, histomonads a clonal culture propagated in vitro either for short period time (21 passages) preserve virulence 295 passages achieve attenuation. On first day life birds each species parasites. Throughout experiment, all examined daily clinical signs attributable infection. Furthermore, excretion viable parasites was...

Collection of samples and serum separation From Faisalabad, a total 1053 children under 10 years age were surveyed from September, 2013 to May, 2014 about vaccination disease history measles. Out these, 182 blood (132 vaccinated 50 non-vaccinated individuals) collected randomly. Similarly during the same period, Jhang, 813 period The was each individual in 2.5 or 5ml disposable syringes. Each sample transferred immediately separate clot activator tube (Lab Vac Gel Clot Activators), kept on...

The efficiency of petroleum ether extracts weed plants, Euphorbia prostrata and Chenopodiastrum murale, for the control Trogoderma granarium was investigated. were prepared in using whole plants. Diet incorporation used mortality bioassay area preference repellency against third instar larvae T . results showed relatively high rate larval after 6 days with extract concentrations 10, 20 30%. At 30% corresponding rates induced by E. C. murale 25%, respectively. Low mortalities obtained both...

Probiotic properties of isolated lactic acid bacteria (LAB) from sustainable foods including camel milk are the potential research domains. For this purpose, samples ( n = 20), four different herds Camelus dromedarius , were processed for identification LAB strains based on biochemical profiles followed by amplification and sequence analysis 16S rRNA gene. The probiotic characteristics, i.e., acids bile salts tolerance, antimicrobial susceptibility profiles, hemolytic activities,...