A5025598471- Multiple Myeloma Research and Treatments
- Bone health and treatments
- Bone Metabolism and Diseases
- TGF-β signaling in diseases
- Liver physiology and pathology
- Cytokine Signaling Pathways and Interactions
- Peptidase Inhibition and Analysis
- Chemokine receptors and signaling
- Chronic Myeloid Leukemia Treatments
- Immune Cell Function and Interaction
- Glycosylation and Glycoproteins Research
- Bone Tissue Engineering Materials
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Bone and Dental Protein Studies
- Titanium Alloys Microstructure and Properties
- Rheumatoid Arthritis Research and Therapies
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Proteoglycans and glycosaminoglycans research
- Extracellular vesicles in disease
- 3D Printing in Biomedical Research
- Mesenchymal stem cell research
- Cancer Mechanisms and Therapy
- GDF15 and Related Biomarkers
Norwegian University of Science and Technology
2015-2024
St Olav's University Hospital
2006-2024
Centre for Inflammation Research
2017
St Vincents Institute of Medical Research
2013-2014
Karolinska Institutet
2002
Lund University
2002
University of Arkansas for Medical Sciences
2002
Vejle Sygehus
2002
PD1/PDL1-directed therapies have been unsuccessful for multiple myeloma (MM), an incurable cancer of plasma cells in the bone marrow (BM). Therefore, other immune checkpoints such as extracellular adenosine and its immunosuppressive receptor should be considered. CD39 CD73 convert ATP to adenosine, which inhibits T-cell effector functions via A2A (A2AR). We set out investigate whether blocking pathway could a therapy MM.Expression on BM from patients proliferation were determined by flow...
Interleukin-6 (IL-6) family cytokines act via gp130 in the osteoblast lineage to stimulate formation of osteoclasts (bone resorbing cells) and activity osteoblasts forming cells), inhibit expression osteocyte protein, sclerostin. We report here that a profound reduction trabecular bone mass occurs both when is deleted entire (Osx1Cre f/f) this deletion restricted osteocytes (DMP1Cre f/f). This was caused not by an alteration osteoclastogenesis, but low level specific compartment. In...
Mineralized biomaterials are promising for use in bone tissue engineering. Culturing osteogenic cells such materials will potentially generate biological grafts that may even further augment healing. Here, we studied differentiation of human mesenchymal stem (MSC) an alginate hydrogel system where the were co-immobilized with alkaline phosphatase (ALP) gradual mineralization microenvironment. MSC embedded unmodified beads and mineralized ALP to a polymer/hydroxyapatite scaffold mimicking...
In this study we set out to investigate whether anti PDL1 or PD–1 treatment targeting the immune system could be used against multiple myeloma. DCs are important in regulating T cell responses tumors. We therefore determined and PDL2 expression on DC populations bone marrow of patients with plasma disorders using multicolour Flow Cytometry. specifically looked at CD141+ CD141- myeloid CD303+ plasmacytoid DC. The majority cells (PC) subpopulations expressed PDL1, but proportion positive PDL1+...
Multiple myeloma (MM) is a hematologic cancer characterized by expansion of malignant plasma cells in the bone marrow. Most patients develop an osteolytic disease, largely caused increased osteoclastogenesis. The marrow hypoxic, and hypoxia may contribute to MM disease progression, including loss. Here we identified interleukin-32 (IL-32) as novel inflammatory cytokine expressed subset primary cell lines. We found that high IL-32 gene expression correlated with inferior survival was higher...
Circulating tumor DNA is a promising biomarker to monitor load and genome alterations. We explored the presence of circulating in multiple myeloma patients its relation disease activity during long-term follow-up. used digital droplet polymerase chain reaction analysis recurrent mutations, mainly mitogen activated protein kinase pathway genes NRAS, KRAS BRAF. Mutations were identified by next-generation sequencing or bone marrow plasma cells, their analyzed 251 archived serum samples...
Angiogenesis is a complex process involved in the proliferation and metastasis of malignant tumours, partly triggered by secretion various angiogenic factors tumour cells or stromal environment. We investigated correlation between bone marrow angiogenesis, estimated as microvessel density (MVD), interleukin-6 (IL-6), basic fibroblastic growth factor (bFGF), hepatocyte (HGF) syndecan-1 67 patients with newly diagnosed multiple myeloma, evaluated prognostic value these parameters. Circulating...
Multiple myeloma is a malignancy of plasma cells predominantly located in the bone marrow. A number morphogenetic proteins (BMPs) induce apoptosis vitro, and with this study we add BMP-9 to list. has been found human serum at concentrations that inhibit cancer cell growth vitro. We here show level was elevated patients (median 176 pg/ml, range 8-809) compared healthy controls 110 8-359). also present marrow able 4 out 11 primary samples by signaling through ALK2. BMP-9-induced associated...
Abstract Background c‐ MET is the tyrosine kinase receptor of hepatocyte growth factor ( HGF ). ‐c‐ signaling involved in many human malignancies, including multiple myeloma MM Recently, agents have been developed directed to interfere at different levels pathway. Nanobodies ® are therapeutic proteins based on smallest functional fragments heavy‐chain‐only antibodies. In this study, we wanted determine anticancer effect a novel anti‐c‐ Nanobody . Methods We examined effects an thymidine...
A multitude of autogenous/allogeneic and semi-synthetic bone graft materials have been developed to reconstruct the defective tissue but with high bio-cost potential environmental pollution. With calcium content several trace elements, chicken eggshells are no longer considered as wastes attractive sources high-value-added biomaterials. This study used synthetic hydroxyapatite (HAp) synthesize amorphous phosphate (ACP) materials, namely Control Eggshell. The physiochemical characteristics,...
Abstract Obesity is associated with an increased risk of developing multiple myeloma (MM). The molecular mechanisms causing this association complex and incompletely understood. Whether obesity affects bone marrow immune cell composition in not characterized. Here, we examined the effect diet-induced on tumor growth a Vk*MYC (Vk12653) transplant model myeloma. We find that promoted spleen reduced relative number T B cells marrow. Our results suggest may reduce MM surveillance thus contribute to MM.
Single-cell technologies offer a unique opportunity to explore cellular heterogeneity in hematopoiesis, reveal malignant hematopoietic cells with clinically significant features and measure gene signatures linked pathological pathways. However, reliable identification of cell types is crucial bottleneck single-cell analysis. Available databases contain dissimilar nomenclature non-concurrent marker sets, leading inconsistent annotations poor interpretability. Furthermore, current tools focus...
Parathyroid hormone (PTH) treatment stimulates osteoblast differentiation and bone formation, is the only currently approved anabolic therapy for osteoporosis. In cells of lineage, PTH also expression members interleukin 6 (IL-6) cytokine superfamily. Although similarity gene targets regulated by these cytokines suggest cooperative action, dependence action on IL-6 signaling unknown. To determine whether in osteocyte through glycoprotein 130 (gp130), common superfamily receptor subunit,...
Abstract Multiple myeloma (MM) is an incurable cancer of terminally differentiated plasma cells that proliferate in the bone marrow. miRNAs are promising biomarkers for risk stratification MM and several shown to have a function disease pathogenesis. However, date, surprisingly few miRNA-mRNA interactions been described functionally validated MM. In this study, we performed miRNA-seq mRNA-seq on CD138 + isolated from marrow aspirates 86 patients identify novel between sRNAs mRNAs. We...
Hepatocyte growth factor (HGF) is a cytokine produced by myeloma cells. We examined serum HGF levels in population of young patients (median age 52 years) treated with high-dose chemotherapy. Sera from 128 at diagnosis and serial samples 16 were analysed. Compared 62 healthy controls, was elevated 25% 0.48 1.08 ng/ml respectively; P < 0.0001). The 95 who completed therapy analysed for the impact on survival. Median survival not reached after 77 months patient group normal values (< 1.7...