A5000119408- Cancer Research and Treatments
- Animal Genetics and Reproduction
- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- Microtubule and mitosis dynamics
- Cancer-related Molecular Pathways
- RNA modifications and cancer
- Pluripotent Stem Cells Research
- Cancer, Hypoxia, and Metabolism
- RNA Research and Splicing
- Genetics, Aging, and Longevity in Model Organisms
- Hippo pathway signaling and YAP/TAZ
- Circadian rhythm and melatonin
- Cancer-related gene regulation
- Cancer Mechanisms and Therapy
- Renal and related cancers
- Biomedical Ethics and Regulation
- Developmental Biology and Gene Regulation
- Peptidase Inhibition and Analysis
- Ubiquitin and proteasome pathways
- Molecular Biology Techniques and Applications
- RNA Interference and Gene Delivery
- Ginger and Zingiberaceae research
- Dietary Effects on Health
- Cancer Genomics and Diagnostics
Hospital Universitario Virgen del Rocío
2016-2024
Consejo Superior de Investigaciones Científicas
2015-2024
Instituto de Biomedicina de Sevilla
2015-2024
Universidad de Sevilla
2015-2024
Instituto de Salud Carlos III
2017-2024
Centro de Investigación Biomédica en Red de Cáncer
2020-2024
Weatherford College
2023
Centro de Investigación Biomédica en Red
2017
Ovarian cancer is one of the most common and malignant cancers, partly due to its late diagnosis high recurrence. Chemotherapy resistance has been linked poor prognosis believed be stem cell (CSC) pool. Therefore, elucidating molecular mechanisms mediating therapy essential finding new targets for therapy-resistant tumors.shRNA depletion MYPT1 in ovarian lines, miRNA overexpression, RT-qPCR analysis, patient tumor samples, line- tumorsphere-derived xenografts, vitro vivo treatments, analysis...
Abstract Background Hypoxia in solid tumors is an important source of chemoresistance that can determine poor patient prognosis. Such relies on the presence cancer stem cells (CSCs), and hypoxia promotes their generation through transcriptional activation by HIF transcription factors. Methods We used ovarian (OC) cell lines, xenograft models, OC samples, databases, induced pluripotent (iPSCs) Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq). Results Here, we show...
Purpose: Cancer stem cells (CSC) are self-renewing tumor cells, with the ability to generate diverse differentiated cell subpopulations. They differ from normal in deregulation of mechanisms that normally control physiology. CSCs origin metastasis and highly resistant therapy. Therefore, understanding CSC deregulated pathways is important for control.Experimental Design: We have included experiments vitro, lines tumors different origins. used patient-derived xenografts (PDX) public...
// José M. García-Heredia 1, 2 , Eva Verdugo Sivianes 1 Antonio Lucena-Cacace Sonia Molina-Pinelo 3 Amancio Carnero Instituto de Biomedicina Sevilla (IBIS), Hospital Universitario Virgen del Rocio, Universidad Sevilla, Consejo Superior Investigaciones Cientificas, Seville, Spain Department of Vegetal Biochemistry and Molecular Biology, University Present address: Investigación 12 Octubre, Madrid, Correspondence to: Carnero, email: acarnero-ibis@us.es Keywords: NumbL, Notch, cancer stem...
Ovarian cancer is the leading cause of gynecologic cancer-related death, due in part to a late diagnosis and high rate recurrence. Primary acquired platinum resistance related low response probability subsequent lines treatment poor survival. Therefore, comprehensive understanding mechanisms that drive urgently needed.We used bioinformatics analysis public databases RT-qPCR quantitate relative gene expression profiles ovarian tumors. Many dysregulated genes were stem cell (CSC) factors, we...
The epicardium is a mesothelial layer covering the myocardium serving as progenitor source during cardiac development. reactivates upon injury supporting repair and regeneration. Fine-tuned balanced signaling regulates cell plasticity cell-fate decisions of epicardial-derived cells (EPCDs) via epicardial-to-mesenchymal transition (EMT). However, powerful tools to investigate epicardial function, including markers with pivotal roles in developmental signaling, are still lacking. Here, we...
Summary Head and neck squamous cell carcinoma (HNSCC) is a heterogeneous group of tumors that affect different anatomical locations. Despite this heterogeneity, HNSCC treatment depends on the location, TNM stage resectability tumor. Classical chemotherapy based platinum-derived drugs (cisplatin, carboplatin oxaliplatin), taxanes (docetaxel, paclitaxel) 5-fluorouracil 1 . advances in treatment, rate tumor recurrence patient mortality remain high. Therefore, search for new prognostic...
The tumor microenvironment may alter the original tumorigenic potential of cells. Under harsh environmental conditions, genetic alterations conferring selective advantages initiate growth subclones, providing new opportunities for these tumors to grow. We performed a loss‐of‐function screen identify able promote cell in absence glucose. identified that downregulation MYBBP1A increases properties under nonpermissive conditions. simultaneously activates PGC1α, directly by alleviating direct...
// Eva M. Verdugo-Sivianes 1, 2 , Lola Navas Sonia Molina-Pinelo Irene Ferrer 2, 3 Alvaro Quintanal-Villalonga Javier Peinado 4 Jose Garcia-Heredia 5 Blanca Felipe-Abrio Sandra Muñoz-Galvan Juan J. Marin 6 Luis Montuenga 7 Paz-Ares and Amancio Carnero 1 Instituto de Biomedicina Sevilla (IBIS), Hospital Universitario Virgen del Rocío, Universidad Sevilla, Consejo Superior Investigaciones Científicas, Spain CIBER Cáncer, Salud Carlos III, Pabellón 11, Planta 0, Madrid, H120-CNIO Lung Cancer...
Tumors are cellular ecosystems where different populations and subpopulations of cells coexist. Among these cells, cancer stem (CSCs) considered to be the origin tumor mass, being involved in metastasis resistance conventional therapies. Furthermore, have an enormous plasticity a phenomenon de-differentiation mature CSCs may occur. Therefore, it is essential identify genetic alterations that cause for future design therapeutic strategies. In this study, we characterized role MYBBP1A by...
Background: As age increases, the risk of developing fragility also increases.Improving knowledge frailty could contribute to maintaining functional ability elderly people.Interleukin (IL)-10 homozygous knockout mice (IL-10 tm/tm [IL10KO]) constitute an excellent tool for study frailty.Because patients with demonstrate overexpression CCR5, rapamycin (RAPA) and/or maraviroc (MVC), two molecules able decrease CCR5 expression, were evaluated.Results: Muscle myostatin was reduced in all...
Non-small-cell lung cancer (NSCLC) is the leading cause of death worldwide, generating an enormous economic and social impact that has not stopped growing in recent years. Cancer treatment for this neoplasm usually includes surgery, chemotherapy, molecular targeted treatments, ionizing radiation. The prognosis terms overall survival (OS) disparate therapeutic responses among patients can be explained, to a great extent, by existence widely heterogeneous profiles. main objective study was...
// Jose Luis Lopez-Guerra 1,2 , Eva M. Verdugo-Sivianes 1 Daniel Otero-Albiol Begoña Vieites 3 Maria J. Ortiz-Gordillo 2 De León 4 Juan Praena-Fernandez 5 Marin 1,6 and Amancio Carnero 1,7 Instituto de Biomedicina Sevilla (IBIS/HUVR/CSIC/Universidad Sevilla), Seville, Spain Department of Radiation Oncology, University Hospital Virgen del Rocío, Pathology, Del Unidad Funcional Patología Mamaria, Methodology Unit- Fundación Pública Andaluza para la Gestión Investigación en Salud Sevilla, Rocío...
Rationale: SPINOPHILIN (SPN, PPP1R9B) is an important tumor suppressor involved in the progression and malignancy of different tumors depending on its association with protein phosphatase 1 (PP1) ability PP1-SPN holoenzyme to dephosphorylate retinoblastoma (pRB).Methods: We performed a mutational analysis SPN human tumors, focusing region interaction PP1 pRB.We explored effect SPN-A566V mutation immortalized non-tumorigenic cell line epithelial breast tissue, MCF10A, two p53-mutated cancer...
Abstract Cellular senescence connects aging and cancer. is a common program activated by cells in response to various types of stress. During this process, lose their proliferative capacity undergo distinct morphological metabolic changes. Senescence itself constitutes tumor suppression mechanism plays significant role organismal promoting chronic inflammation. Additionally, age one the major risk factors for developing breast Therefore, while can suppress development early life, it also...
Cellular senescence and low-grade inflammation favor the acceleration of aging. The liver is an essential metabolic organ because changes related to its function are age-related diseases. objective this study was evaluate effects maraviroc (MVC) and/or rapamycin (RAPA) on tissue in experimental model frailty syndrome mice, since MVC RAPA two molecules able decrease CCR5 expression, which overexpressed patients with frailty. Methods: Eighty male homozygous IL10KO mice were randomly assigned...
Summary Hypoxia in solid tumors is a source of chemoresistance that determines poor patient prognosis and relies on the presence cancer stem cells (CSCs). Here we use ovarian (OC) as model combination 2D 3D cell cultures, xenograft models, samples, transcriptional databases, iPSCs ATAC-seq, to address mechanisms leading hypoxia-induced CSC generation chemoresistance. We show hypoxia activates expression PLD2 gene encoding phospholipase D2. overexpression leads increased CSC-like features,...