A5063985660- Cancer Research and Treatments
- Cancer-related Molecular Pathways
- PI3K/AKT/mTOR signaling in cancer
- Ubiquitin and proteasome pathways
- Peptidase Inhibition and Analysis
- Epigenetics and DNA Methylation
- Microtubule and mitosis dynamics
- Telomeres, Telomerase, and Senescence
- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- Cancer Mechanisms and Therapy
- Virus-based gene therapy research
- RNA modifications and cancer
- X-ray Diffraction in Crystallography
- Protein Kinase Regulation and GTPase Signaling
- Crystallization and Solubility Studies
- Signaling Pathways in Disease
- Genetics, Aging, and Longevity in Model Organisms
- Metal complexes synthesis and properties
- Animal Genetics and Reproduction
- RNA Interference and Gene Delivery
- Fibroblast Growth Factor Research
- MicroRNA in disease regulation
- Heat shock proteins research
- Cancer-related molecular mechanisms research
Hospital Universitario Virgen del Rocío
2016-2025
Universidad de Sevilla
2016-2025
Instituto de Biomedicina de Sevilla
2016-2025
Anhui Medical University
2025
Consejo Superior de Investigaciones Científicas
2015-2024
Centro de Investigación Biomédica en Red de Cáncer
2016-2024
Instituto de Salud Carlos III
2005-2024
Spanish National Cancer Research Centre
2004-2023
Centro de Investigación Biomédica en Red
2017-2021
Kyoto University
2018
p53 has a key role in the negative regulation of cell proliferation, maintenance genomic stability, and suppression transformation tumorigenesis. To identify novel regulators p53, we undertook two functional screens to isolate genes which bypassed either p53-mediated growth arrest or apoptosis. In both screens, isolated cDNAs encoding macrophage migration inhibitory factor (MIF), cytokine that was shown previously exert local systemic proinflammatory activities. Treatment with MIF overcame...
An unbiased screen for genes that can immortalize mouse embryonic fibroblasts identified the glycolytic enzyme phosphoglycerate mutase (PGM). A 2-fold increase in PGM activity enhances flux, allows indefinite proliferation, and renders cells resistant to ras-induced arrest. Glucosephosphate isomerase, another enzyme, displays similar and, conversely, depletion of or glucosephosphate isomerase with short interfering RNA triggers premature senescence. Immortalized stem display higher flux more...
(R)-Roscovitine (CYC202) is often referred to as a "selective inhibitor of cyclin-dependent kinases." Besides its use biological tool in cell cycle, neuronal functions, and apoptosis studies, it currently evaluated potential drug treat cancers, neurodegenerative diseases, viral infections, glomerulonephritis. We have investigated the selectivity (R)-roscovitine using three different methods: 1) testing on wide panel purified kinases that, along with previously published data, now reaches 151...
Abstract Purpose: Colorectal cancer is the second most common in women and third men worldwide. However, despite current progress, many patients with advanced metastatic tumors still die from malignancy. Refractory disease often relies on nicotinamide adenine dinucleotide (NAD)-dependent mechanisms. NAD metabolism a stable regeneration circuit are required to maintain tissue homeostasis metabolism. high levels of confer therapy resistance tumors. Experimental Design: Ectopic overexpression...
The purpose of this study was to determine the global patterns aberrant DNA methylation in thyroid cancer.We have used arrays determine, for first time, genome-wide promoter status papillary, follicular, medullary, and anaplastic tumors.We identified 262 352 hypermethylated 13 21 hypomethylated genes differentiated papillary follicular tumors, respectively. Interestingly, other tumor types analyzed displayed more (280 393 medullary tumors) than aberrantly (86 131 tumors). Among indentified,...
Lung adenocarcinoma and chronic obstructive pulmonary disease (COPD) are diseases that share common aetiological factors (tobacco smoking) probable dysregulated pathways. MicroRNAs (miRNAs) play an essential role in regulating numerous physiological pathological processes. The purpose of this study was to assess global miRNA expression patterns patients with COPD and/or elucidate distinct regulatory networks involved the pathogenesis these two smoking-related diseases. Expression 381 miRNAs...
Ovarian cancer is one of the most common and malignant cancers, partly due to its late diagnosis high recurrence. Chemotherapy resistance has been linked poor prognosis believed be stem cell (CSC) pool. Therefore, elucidating molecular mechanisms mediating therapy essential finding new targets for therapy-resistant tumors.shRNA depletion MYPT1 in ovarian lines, miRNA overexpression, RT-qPCR analysis, patient tumor samples, line- tumorsphere-derived xenografts, vitro vivo treatments, analysis...