A5076001164- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Genomics and Phylogenetic Studies
- Plant Pathogens and Fungal Diseases
- bioluminescence and chemiluminescence research
- Photoreceptor and optogenetics research
- Coleoptera: Cerambycidae studies
- Molecular Biology Techniques and Applications
- Coleoptera Taxonomy and Distribution
- Plant biochemistry and biosynthesis
- Insect and Pesticide Research
- Enzyme Production and Characterization
- Plant Disease Resistance and Genetics
- Forest Insect Ecology and Management
- Reproductive System and Pregnancy
- Plant Reproductive Biology
- Biofuel production and bioconversion
- Plant Stress Responses and Tolerance
- Parasitic Diseases Research and Treatment
- Plant tissue culture and regeneration
- Animal Genetics and Reproduction
- Mycobacterium research and diagnosis
- Lepidoptera: Biology and Taxonomy
- Gene expression and cancer classification
- Chromosomal and Genetic Variations
University of Georgia
2012-2023
Environmental Protection Agency
2003
Philipps University of Marburg
1999
University of Iowa
1996
Deutsche Ophthalmologische Gesellschaft
1967
Deutschen Ornithologen-Gesellschaft e.V.
1967
Abstract Background The size and complexity of conifer genomes has, until now, prevented full genome sequencing assembly. large research community economic importance loblolly pine, Pinus taeda L., made it an early candidate for reference sequence determination. Results We develop a novel strategy to the pine that combines unique aspects reproductive biology assembly methodology. use whole shotgun approach relying primarily on next generation generated from single haploid seed...
Renilla reniformis is an anthozoan coelenterate capable of exhibiting bioluminescence. Bioluminescence in results from the oxidation luciferin (coelenterazine) by luciferase [Renilla-luciferin:oxygen 2-oxidoreductase (decarboxylating), EC 1.13.12.5]. In vivo, excited state luciferin-luciferase complex undergoes process nonradiative energy transfer to accessory protein, green fluorescent which vitro, emits blue light absence any protein. A cDNA library has been constructed lambda gt11 and...
Abstract Background Global transcriptional analysis of loblolly pine ( Pinus taeda L.) is challenging due to limited molecular tools. PtGen2, a 26,496 feature cDNA microarray, was fabricated and used assess drought-induced gene expression in propagule roots. Statistical differential weighted correlation network were identify drought-responsive genes further characterize the basis drought tolerance pine. Results Microarrays interrogate root populations obtained from 12 genotype × treatment...
The infectious yeast Candida albicans progresses through two developmental programs which involve differential gene expression, the bud-hypha transition and high-frequency phenotypic switching. To understand how differentially expressed genes are regulated in this organism, promoters of phase-specific must be functionally characterized, a bioluminescent reporter system would facilitate such characterization. However, C. has adopted nontraditional codon strategy that involves tRNA with CAG...
ABSTRACT A gene ( yacK ) encoding a putative multicopper oxidase (MCO) was cloned from Escherichia coli , and the expressed enzyme demonstrated to exhibit phenoloxidase ferroxidase activities. The purified protein contained six copper atoms per polypeptide chain displayed optical electron paramagnetic resonance (EPR) spectra consistent with presence of type 1, 2, 3 centers. strong 610 (Ε = 10,890 M −1 cm stoichiometry were taken as evidence that, similar ceruloplasmin, likely contains...
Abstract Background Phenylalanine ammonia lyase (PAL) is a key enzyme of the phenylpropanoid pathway that catalyzes deamination phenylalanine to trans-cinnamic acid, precursor for lignin and flavonoid biosynthetic pathways. To date, PAL genes have been less extensively studied in gymnosperms than angiosperms. Our interest stems from their potential role defense responses Pinus taeda , especially with respect lignification production low molecular weight phenolic compounds under various...
Wood formation has been studied extensively at the cellular and biochemical levels, but remains poorly understood with respect to gene expression regulation. As a first step toward identifying genes specifically involved in wood characterizing their roles determining quality, serial analysis of (SAGE) was used quantify lignifying xylem from single, 10-year-old loblolly pine (Pinus taeda L.). Two SAGE libraries were generated based on isolated either upper (crown) or lower (base) portions...
Abstract Serial analysis of gene expression was used to profile transcript levels in Arabidopsis roots and assess their responses 2,4,6-trinitrotoluene (TNT) exposure. SAGE libraries representing control TNT-exposed seedling root transcripts were constructed, each sequenced a depth roughly 32,000 tags. More than 19,000 unique tags identified overall. The second most highly induced tag (27-fold increase) represented glutathione S-transferase. Cytochrome P450 enzymes, as well an ABC...
The industrial application of thermophilic (eu)bacteria is hampered by the lack genetic systems for these bacteria. We report here first unequivocal transformation a Gram-positive, thermophilic, anaerobic microorganism, Thermoanaerobacterium, with kanamycin resistance-mediating plasmid pIKM1. construct pIKM1 based on Escherichia coli–Clostridium acetobutylicum shuttle vector pIMP1 and contains thermostable cassette from S. faecalis pKD102. Using electrotransformation, mediated resistance in...
Drought stress is the principal cause of seedling mortality in pine forests southeastern United States and many other forested regions around globe. As part a larger effort to discover loblolly genes, this study subjected rooted cuttings three unrelated genotypes watering regimens. Expressed sequence tags (ESTs) were obtained from both 3' 5' ends 12,918 randomly selected cDNAs generated root tissues. These ESTs clustered identify 6,765 unique transcripts (UniScripts) derived 6,202 putative...
Douglas-fir (Pseudotsuga menziesii), one of the most economically and ecologically important tree species in world, also has largest breeding programs. Although coastal interior varieties (vars. menziesii glauca) are native to North America, variety is widely planted for timber production Europe, New Zealand, Australia, Chile. Our main goal was develop a SNP resource large enough facilitate genomic selection To accomplish this, we developed 454-based reference transcriptome Douglas-fir,...
A cDNA encoding the Renilla reniformis luciferase was expressed in similan and murine cells a transient stable manner, respectively. Light emission catalyzed by detected from transfected both vitro vivo. This work establishes gene as new efficient marker of expression mammalian cells.
Abstract Background It is during embryogenesis that the plant body plan established and meristems responsible for all post-embryonic growth are specified. The molecular mechanisms governing conifer still largely unknown. Their elucidation may contribute valuable information to clarify if distinct features of embryo development in angiosperms gymnosperms result from differential gene regulation. To address this issue, we have performed first transcriptomic analysis zygotic a species ( Pinus...
During their lifetime, conifer trees are exposed to numerous herbivorous insects. To protect themselves against pests, have developed a broad repertoire of protective mechanisms. Many the plant's defence reactions activated upon an insect attack, and underlying regulatory mechanisms not entirely understood yet, in particular trees. Here, we present results our studies on transcriptional response volatile compounds production Scots pine (Pinus sylvestris) large weevil (Hylobius abietis)...
The genes encoding acetyl xylan esterase 1 (axe1) and a beta-xylosidase (xylB) have been cloned sequenced from Thermoanaerobacterium sp. strain JW/SL YS485. axe1 is located 22 nucleotides 3' of the xylB sequence. identity was confirmed by comparison deduced amino acid sequence to peptide analysis data purified 1. gene identified expression cloning homology known beta-xylosidases. Plasmids which independently expressed either (pAct1BK) or (pXylo-1.1) were constructed in Escherichia coli....
During oviposition, female Sirex noctilio (F.) (Siricidae) woodwasps inject their conifer hosts with a venom gland secretion. The secretion induces variety of host physiological changes that facilitate subsequent lethal infection by symbiotic fungus. A heat-stable factor can migrate from the site oviposition in trunk through xylem to needles crown attacked pines was purified size-fractionation and reversed-phase-high-performance liquid chromatography using activity assays based on defense...
Genital C. trachomatis infection may cause pelvic inflammatory disease (PID) that can lead to tubal factor infertility (TFI). Understanding the pathogenesis of chlamydial complications including pathophysiological processes within female host genital tract is important in preventing adverse pathology. MicroRNAs regulate several infectious and non-infectious etiologies. In this study, we tested hypothesis miRNA profile single repeat infections will be different these differences time...
Many therapeutic monoclonal antibodies require binding to Fc γ receptors (FcγRs) for full effect and increasing the affinity increases efficacy. Preeminent among five activating human FcγRs is FcγRIIIa/CD16a expressed by natural killer (NK) cells. CD16a heavily processed, recent reports indicate that composition of asparagine(N)-linked carbohydrates (glycans) impacts affinity. These observations specific manipulation N-glycan in CD16a-expressing effector cells including NK may improve...
Two closely related isolates, 27335T and 24999, of rapidly growing, non-pigmented mycobacteria, were cultured from two clinically ill fish the family Syngnathidae. Whole genome sequencing isolates revealed low sequence homology to documented mycobacteria within public databases such as NCBI. Evaluation targeted housekeeping genes, including 16S rRNA, ITS, rpoB hsp65, bacteria distantly Mycobacterium senegalense CK2 M4421 farcinogenes DSM 43637. Phenotypic, biochemical dDNA-DNA hybridization...
Mycobacterium chelonae is a member of the chelonae-abscessus complex and cause opportunistic disease in fish, reptiles, birds, mammals including humans. Isolates are often difficult to identify have differing antimicrobial susceptibilities. Thirty-one previously identified rapidly-growing, non-tuberculous sp. isolates cultured from biofilms, mammals, humans, three ATCC reference strains were evaluated with nine M. whole genome sequences GenBank by phylogenomic analysis, targeted gene...
Tissues isolated from conifer species, particularly those belonging to the Pinaceae family, such as loblolly pine (Pinus taeda L.), contain high concentrations of phenolic compounds and polysaccharides that interfere with RNA purification. Isolation high-quality these species requires rigorous tissue collection procedures in field employment an isolation protocol comprised multiple organic extraction steps order isolate sufficient quality for microarray other genomic analyses. The...