Glutamate oxaloacetate transaminase 1 (GOT1) regulates cellular metabolism through coordinating the utilization of carbohydrates and amino acids to meet nutrient requirements. KRAS mutated cancer cells were recently shown rely on GOT1 support long-term cell proliferation. The aim present study was address role in metabolic adaption cells.GOT1-null knockdown lines established CRISPR/Cas9 shRNA techniques. growth properties, colony formation ability, autophagy selected gene expression profiles...

// Xiaoshan Zhou, João A. Paredes, Shuba Krishnan, Sophie Curbo, Anna Karlsson Division of Clinical Microbiology, Department Laboratory Medicine, Karolinska Institute, University Hospital Huddinge, 141 86 Stockholm, Sweden Correspondence to: Karlsson, e-mail: anna.karlsson@ki.se Keywords: oxidative stress, redox homeostasis, ROS, SLC25A10 Received: December 18, 2014 Accepted: February 11, 2015 Published: March 09, ABSTRACT Dysregulation cell metabolism is critical for the growth properties...

Abstract Adult neurogenesis, the production of newborn neurons from neural stem cells (NSCs) has been suggested to be decreased in patients with schizophrenia. A similar finding was observed an animal model schizophrenia, as indicated by bromodeoxyuridine (BrdU) labelling response a non-competitive N-methyl-d-aspartate (NMDA) receptor antagonist. The antipsychotic drug clozapine shown counteract decrease BrdU-labelled hippocampal dentate gyrus (DG). However, phenotypic determination...

Thymidine kinase 2 (TK2) deficiency in humans leads to a myopathic form of mitochondrial DNA (mtDNA) deficiency. Here we present skeletal and cardiac muscle specific TK2 knockout mouse (mTk2 KO). The mice showed dilated hearts markedly reduced adipose tissue during week 12 16. A severe decrease mtDNA was found only heart mTk2 KO mice. Expression analysis key metabolic genes 16 weeks significant changes involved lipid metabolism, with different patterns muscle. Our study further suggests that...

Background The entry of HIV into its host cell is an interesting target for chemotherapeutic intervention in the life-cycle virus. During entry, reduction disulfide bridges viral envelope glycoprotein gp120 by cellular oxidoreductases crucial. thioredoxin reductase-1 plays important role this oxidoreduction process recycling electrons to thioredoxin-1. Therefore, inhibitors may inhibit during HIV-1 entry. In present study, tellurium-based were investigated as potential Results...

Thymidine kinase 2 (TK2) deficiency in humans causes mitochondrial DNA (mtDNA) depletion syndrome. To study the molecular mechanisms underlying disease and search for treatment options, we previously generated described a TK2 deficient mouse strain (TK2−/−) that progressively loses its mtDNA. The TK2−/− model displays symptoms similar to harboring infantile fatal encephalomyopathy. Here, have studied clarify pathological role of progressive mtDNA liver severe outcome deficiency. We observed...

Abstract Deoxyguanosine kinase (DGUOK) provides guanosine and adenosine nucleotides for mitochondrial DNA (mtDNA) replication, its deficiency in humans leads to hepatocerebral mtDNA depletion syndrome or isolated hepatic disease. There are poor treatment options DGUOK the aim of this study was generate a model further studies disease that could reveal novel strategies. We report Dguok-deficient mouse strain that, similar humans, is most severely affected liver. The Dguok complete knockout...

Abstract Human immunodeficiency virus (HIV-1) entry is initiated by the binding between viral envelope glycoprotein gp120 and host receptor CD4, followed reduction of structural disulfides CD4. The thioredoxin-1 (Trx1) efficiently reduces CD4 in vitro , recently CD4-dependent HIV-1 was shown to be inhibited anti-Trx1-antibodies, indicating a central role for Trx1. 1-methylpropyl-2-imidazolyl disulfide (PX-12) reversible inhibitor Trx1 system that may also cause slow irreversible...

Many educational institutions transitioned to digital distance-based learning and assessment formats in 2020 due the severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) pandemic. This shift has often been associated with concerns about increased student cheating heightened stress. In this study, we compared major course grades of students a microbiology delivered through format, including take-home examination

ChatGPT (GPT-3.5) has entered higher education and there is a need to determine how use it effectively. This descriptive study compared the ability of GPT-3.5 teachers answer questions from dental students construct detailed intended learning outcomes. When analyzed according Likert scale, we found that answered in similar or even more elaborate way answers had previously been provided by teacher. was also asked outcomes for course microbial pathogenesis, when these were scale they were,...

Thioredoxin-1 (Trx1) is a protein antioxidant involved in major cellular processes. Increased plasma levels of Trx1 have been associated with human diseases suggesting that marker for oxidative stress putative clinical use. However, the reported mean control cohorts vary hundred-fold between studies (0.8–87 ng/ml), possibly due to methodological differences capture ELISA used different studies. The aim this study was investigate aspects related measurement Trx1. ELISAs utilizing and...

Purpose: It aims to find students’ performance of and perspectives on an objective structured practical examination (OSPE) for assessment laboratory preclinical skills in biomedical science (BLS). also investigate the perception, acceptability, usefulness OSPE from examiners’ point view.Methods: This was a longitudinal study implement BLS. The student group consisted 198 BLS students enrolled semester 4, 2015–2019 at Karolinska University Hospital Huddinge, Sweden. Fourteen teachers...

The global protein thiol pool has been reported to play a major role in the defense against oxidative stress as redox buffer similar glutathione. present study uses novel method visualize cellular changes of response induced stress. Unexpectedly, results showed an uneven distribution thiols resting cells with no apparent change their level or diamide previously. Further analysis revealed that oxidation is artificially high due insufficient activity widely used sample quencher trichloroacetic...

Deoxyguanosine kinase (DGUOK) deficiency causes mtDNA depletion and mitochondrial dysfunction. We reported long survival of DGUOK knockout (Dguok-/-) mice despite low (<5%) content in liver tissue. However, the molecular mechanisms enabling extended remain unknown. Using transcriptomics, proteomics metabolomics followed by vitro assays, we aimed to identify pathways involved Dguok-/- mice. At early stage, serine synthesis folate cycle were activated but declined later. Increased activity...