A5042664406- Cervical Cancer and HPV Research
- Virus-based gene therapy research
- Cell death mechanisms and regulation
- Cancer-related Molecular Pathways
- Molecular Biology Techniques and Applications
- DNA Repair Mechanisms
- RNA Research and Splicing
- Hepatitis B Virus Studies
- Carcinogens and Genotoxicity Assessment
- Ubiquitin and proteasome pathways
- Sphingolipid Metabolism and Signaling
- RNA Interference and Gene Delivery
- interferon and immune responses
- NF-κB Signaling Pathways
- Cancer Cells and Metastasis
- CAR-T cell therapy research
- Adipokines, Inflammation, and Metabolic Diseases
- Lipid Membrane Structure and Behavior
- Adipose Tissue and Metabolism
- Head and Neck Cancer Studies
- Cancer-related gene regulation
- Peptidase Inhibition and Analysis
- Histone Deacetylase Inhibitors Research
- COVID-19 Clinical Research Studies
- T-cell and Retrovirus Studies
Loma Linda University
2014-2024
Loma Linda University Medical Center
2020
City Of Hope National Medical Center
2020
Loma Linda University Health Care
2012
Office of Basic Energy Sciences
2011
Wellesley College
2004
Zhejiang University
2004
Ministry of Education of the People's Republic of China
2004
Georgia State University
1999-2003
Emory University
1987-1996
A complementary DNA (cDNA) for ubiquitin carboxyl-terminal hydrolase isozyme L3 was cloned from human B cells. The cDNA encodes a protein of 230 amino acids with molecular mass 26.182 daltons. is very similar to the bovine homolog, only three differing in over 100 residues compared. acid sequence deduced 54% identical that neuron-specific PGP 9.5. Purification 9.5 confirmed it also hydrolase. These results suggest family such related proteins exists and their expression tissue-specific.
The rat has been used extensively as a model for evaluating chemical toxicities and understanding drug mechanisms. However, its transcriptome across multiple organs, or developmental stages, not yet reported. Here we show, part of the SEQC consortium efforts, comprehensive transcriptomic BodyMap created by performing RNA-Seq on 320 samples from 11 organs both sexes juvenile, adolescent, adult aged Fischer 344 rats. We catalogue expression profiles 40,064 genes, 65,167 transcripts, 31,909...
Ceramide has been suggested to participate in the neuronal cell death that leads Alzheimer's disease (AD), but its role is not yet well-understood. We compared levels of six ceramide subspecies, which differ length their fatty acid moieties, brains from patients who suffered AD, other neuropathological disorders, or both. found elevated Cer16, Cer18, Cer20, and Cer24 with any tested neural defects. Moreover, were highest more than one neuropathologic abnormality. Interestingly, range values...
HPV-DNA integration into cellular chromatin is usually a necessary event in the pathogenesis of HPV-related cancer; however, mechanism has not been clearly defined. Breaks must be created both host DNA and circular viral episome for to occur, studies have shown that indeed increased by induction double strand breaks. Inflammation generates reactive oxygen species, which turn potential create such It plausible these breaks enable greater frequency integration, this way contribute...
ABSTRACT High-risk types of human papillomavirus (HPV) are the causative agents virtually all cases cervical cancer and a significant proportion other anogenital cancers, as well both oral pharyngeal cancers. The high-risk encode two viral oncogenes, E6 E7, which work together to initiate cell transformation. Multiple steps involving activities interactions cellular proteins involved in progression from HPV infection transformation cancer. oncoprotein is expressed several isoforms:...
High risk strains of human papillomavirus (HPV), such as HPV 16, cause cervical carcinoma. The E6 protein 16 mediates the rapid degradation p53, although this is not only function and cannot completely explain its transforming potential. Previous work in our laboratory has demonstrated that transfection into tumor necrosis factor (TNF)-sensitive LM cell line protects expressing cells from TNF-induced apoptosis a p53-independent manner, purpose study was to determine molecular mechanism...
High risk strains of human papillomavirus (HPV), such as HPV 16, cause cervical carcinoma. The E6 protein 16 mediates the rapid degradation tumor suppressor p53, although this is not only function and cannot completely explain its transforming potential. Previous work in our laboratory has demonstrated that can protect cells from necrosis factor-induced apoptosis by binding to C-terminal end factor R1, thus blocking apoptotic signal transduction. In study, was shown also induced via Fas...
We have reported that the E3 14,700-dalton protein (E3 14.7K protein) protects adenovirus-infected mouse C3HA fibroblasts against lysis by tumor necrosis factor (TNF) (L. R. Gooding, L. W. Elmore, A. E. Tollefson, H. Brady, and S. M. Wold, Cell 53:341-346, 1988). also observed E1B 19K human but not cells TNF Aquino, P. J. Duerksen-Hughes, D. Day, T. Horton, Yei, Virol. 65:3083-3094, 1991). now report that, in absence of 14.7K, 10.4K 14.5K proteins are both required to protect C127 as well...
Tumor necrosis factor (TNF) is a multifunctional immunoregulatory protein that secreted by activated macrophages and believed to have antiviral activities. We reported earlier when mouse C3HA fibroblasts are infected with human adenoviruses, the 289R 243R proteins encoded region E1A render cells susceptible lysis TNF, 14,700-molecular-weight (14.7K protein) E3 protects against TNF. now report 19,000-molecular-weight (19K) (176R) E1B transcription unit can protect HEL-299 ME-180 cervical...
TNF is a multifunctional protein that secreted by activated macrophages and believed to have antiviral properties. We reported previously certain murine fibroblasts infected with group C human adenoviruses become sensitive cytolysis TNF, 14,700 m.w. (14.7K) encoded the E3 transcription unit protects cells from cytolysis. now report mapping of adenovirus genes induce sensitivity Experiments using hydroxyurea or 1-beta-D-arabinofuranosylcytosine showed conferred an early gene. Further was done...
ABSTRACT Human papillomavirus type 16 (HPV-16) has developed numerous ways to modulate host-initiated immune mechanisms. The HPV-16 E6 oncoprotein, for example, can the cellular level, and consequently activity, of procaspase 8, thus modifying response cytokines tumor necrosis factor family. from HPV-16, but not low-risk types 6b 11, alters level 8 in a dose-dependent manner. Both large small (E6*) isoforms E6, which originate by way alternate splicing, stability. Intriguingly, although both...
We have previously described a 14,700 m.w. protein (14.7K) encoded by the E3 region of adenovirus that prevents TNF-mediated cytolysis adenovirus-infected C3HA mouse fibroblasts. In studies here we extended our analysis TNF cells and circumstances under which 14.7K protects these from cytolysis. were killed in presence inhibitors synthesis, cytochalasin E (which disrupts microfilaments), when E1A was expressed. As for other cell types, pretreatment with prevented plus cycloheximide or E,...
Treatment of advanced and relapsed cervical cancer is frequently ineffective, due in large part to chemoresistance. To examine the pathways responsible, we employed carcinoma-derived SiHa CaSki cells as cellular models resistance sensitivity, respectively, treatment with chemotherapeutic agents, doxorubicin, cisplatin. We compared proteomic profiles identified potential contribute differential response. then extended these findings by comparing expression level genes involved reactive oxygen...
In vivo carcinogenicity testing is an expensive and time-consuming process, as a result, only relatively small fraction of new existing chemicals has been tested in this manner. Therefore, the development validation alternative approaches desirable. We previously developed mammalian vitro assay for genotoxicity based on ability cells to increase their level tumor-suppressor protein p53 response DNA damage. Cultured are treated with various amounts test substances, at defined times following...