Methylobacterium species frequently inhabit plant surfaces and are able to utilize the methanol emitted from plants as carbon energy sources. As some of known promote growth, significant attention has been paid mechanism growth promotion specificity plant–microbe interactions. By screening our isolate collection for high effect in vitro, we selected candidates field pot tests rice barley, respectively. We found that inoculation resulted better ripening seeds, increased size barley grains but...

Methylobacterium species are ubiquitous α-proteobacteria that reside in the phyllosphere and fed by methanol is emitted from plants. In this study, we applied whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis (WC-MS) to evaluate diversity of collected a variety The WC-MS spectrum was reproducible through two weeks cultivation on different media. peaks M. extorquens strain AM1 cells were attributed ribosomal proteins, but those not also found. We...

The pink-pigmented facultative methylotrophs (PPFMs), a major bacterial group found in the plant phyllosphere, comprise two genera: Methylobacterium and Methylorubrum. They have been separated into three clades: A, B (Methylorubrum), C. Within these genera, however, some species lack either pigmentation or methylotrophy, which raises question of what actually defines PPFMs. present study employed comprehensive comparative genomics approach to reveal phylogenetic relationship among PPFMs...

  Lilium candidum L. is a species which grows in the South West Anatolia region of Turkey. It bulbous plant with beautifully scented flowers and used floral industry. The bulbs are produced by using traditional propagation and in vitrotechniques. Micropropagation rapid technique, but greatest problem contamination fungi bacteria. Antibiotic fungicide treatments were done after sterilization for micropropagation of L. candidum. Fungal contaminants formed during culture...

Five isolates, designated TA2, TA4, TA25(T), KOx(T) and NS15(T) were isolated in previous studies by enrichment mineral medium with potassium oxalate as the sole carbon source characterized using a polyphasic approach. The isolates Gram-reaction-negative, aerobic, non-spore-forming rods. Phylogenetic analyses based on 16S rRNA DNA gyrase B subunit (gyrB) gene sequences confirmed that belonged to genus Pandoraea most closely related sputorum pnomenusa (97.2-99.7 % sequence similarity). could...

Recently, it has been found that two types of methanol dehydrogenases (MDHs) exist in Gram-negative bacterial methylotrophs, calcium-dependent MxaFI-MDH and lanthanide-dependent XoxF-MDH the latter is more widespread genomes. We aimed to isolate characterize methylotrophs. The growth strain La2-4T on methanol, which was isolated from rice rhizosphere soil, strictly lanthanide dependent. Its 16S rRNA gene sequence showed only 93.4% identity Methylophilus luteus MimT , name Novimethylophilus...

Two strains of aerobic, non-spore-forming, Gram-negative, rod-shaped bacteria (ND5 and MY14(T)), previously isolated from urban soil using the membrane-filter enrichment technique, were characterized. Analysis their 16S rRNA gene sequence grouped ND5 MY14(T) within family Oxalobacteraceae (Betaproteobacteria). The highest pairwise similarities for strain found with members genus Herminiimonas, namely Herminiimonas saxobsidens NS11(T) (99.8%) glaciei UMB49(T) (99.6%). Although some fatty acid...

A pink-pigmented, facultatively methylotrophic bacterium, strain 35a(T), was isolated from the leaves of Oxalis corniculata. Cells 35a(T) were Gram-reaction-negative, motile, non-spore-forming rods. The highest 16S rRNA gene pairwise sequence similarities for found with strains Methylobacterium iners 5317S-33(T) (96.7%), 'Methylobacterium soli' YIM 48816 (96.6%) and jeotgali S2R03-9(T) (96.3%). type all other recognized species genus below 96%. Major cellular fatty acids C(18:1)ω7c, C(18:0)...

The current study presents an evaluation of the laboratory instructional tasks prepared based on innovative teaching approaches (research-inquiry, problem solving, project, argumentation and web-based interdisciplinary learning approaches) designed to enhance science teachers’ biotechnology knowledge, awareness experiences. developed by researchers aim improve experiences, as well support through approaches. For this purpose, in-service training course titled Biotechnology Education...

A new lanthanide (Ln3+)-dependent methanol-utilizing bacterial strain, La3113T, was isolated from rice field soil and its taxonomic position investigated using polyphasic approaches. The strain aerobic, Gram-stain-negative, strongly motile, catalase-positive cytochrome oxidase-positive. It could neither catalyse the hydrolysis of urea nor reduce nitrate to nitrite. Growth observed within a temperature range 10-40 °C pH 6-8, with optimum growth at 28 7. Methylamine utilized as single source...

A novel facultatively methanol-utilizing bacterial strain, SM30T, was isolated from rice rhizosphere. Strain SM30T Gram-stain-negative, aerobic, motile, short rods, and grew optimally at pH 7 28 °C. It could tolerate 0 to 2 % (w/v) NaCl. Based on 16S rRNA gene sequence comparisons, strain most closely related Pleomorphomonas oryzae DSM 16300T, with a low similarity of 94.17 %. One the lanthanide metals, lanthanum, enhance its growth slightly methanol. Phylogenetic trees, based mxaF, xoxF...

A Gram-negative, rod-shaped, non-spore-forming bacterium (strain NS11T) was isolated from a lichen-colonized rock surface. On the basis of 16S rRNA gene sequence similarity, strain NS11T shown to belong Betaproteobacteria, and most closely related Herminiimonas arsenicoxydans ULPAs1T (98.8%), aquatilis CCUG 36956T (98.0%) fonticola S-94T (98.0%). Major whole-cell fatty acids were C16:0, C17:0 cyclo C16:1omega7c. Strain also contained high proportions C10:0 3-OH C18:1omega7c. This pattern is...

In the present study, a questionnaire was administered to primary school students (n=836) from 17 different schools determine their basic knowledge, opinions and risk perceptions concerning microorganism. The survey results showed that, 53% (n=475) of defined microorganism as dirt, pollutant, harmful. When they were asked give some examples microorganisms, 37.4% not able any examples. Most think that risks microorganisms are more than benefits, stated feel bad when hear word microbe or light...

Three strains of aerobic, Gram-negative, rod-shaped, non-spore-forming, yellow-pigmented bacteria (OD1(T), YOx(T) and NS13), which were isolated in previous studies by enrichment a mineral medium with potassium oxalate as the sole carbon source, characterized. On basis 16S rRNA gene sequence similarity, OD1(T), NS13 belong to Betaproteobacteria, most closely related Oxalicibacterium flavum TA17(T) (97.2-99.7% similarity). The major whole-cell fatty acids C(16:0), C(16:1)omega7c C(17:0)...

Teachers' perceptions and knowledge concerning nanotechnologies can influence their approach to science-technology teaching behaviors. The purpose of the present study is gain insight into Science Technology teachers (n = 24) teacher candidates' 147) basic opinions about risk perception nanotechnology. In study, nanotechnology questionnaire consisting 9 items was used. analysis data, descriptive interpretive statistical methods were general, it concluded from results that they have...

A pink-pigmented, facultatively methylotrophic bacterium, strain 23e T , was isolated from the leaves of Gnaphalium spicatum (cudweed). The cells were Gram-reaction negative, motile and non-spore-forming rods. On basis 16S rRNA gene sequence similarities, related to Methylobacterium organophilum ATCC 27886 (97.1 %) marchantiae JT1 (97 %), phylogenetic similarities all other species with validly published names less than 97 %. Major cellular fatty acids C 18 : 1 ω7 c 16 00 0 . results DNA–DNA...

A Gram-negative, pleomorphic, rod-shaped, non-spore-forming bacterium, designated strain NS05T, was isolated from soil after enrichment with oxalate. On the basis of 16S rRNA gene sequence similarity, NS05T shown to be phylogenetically related genera Ancylobacter, Starkeya and Angulomicrobium (96.3-98.1% similarity), class Alphaproteobacteria. Strain most closely Ancylobacter rudongensis AS 1.1761T (98.1% similarity). The whole-cell fatty acid pattern typical those found in members genus...

A gram-negative, motile, non-spore-forming rod, designated NS12(T), was isolated from macerated petioles of Rumex sp. after enrichment with oxalate. On the basis 16S rRNA gene sequence similarity, strain NS12(T) phylogenetically related to genera Azorhizobium and Xanthobacter in class Alphaproteobacteria. Strain most closely doebereinerae BR 5401(T) caulinodans ORS 571(T) (98.3 97.3 % respectively). Membership genus excluded by phenotypic characterization. The whole-cell fatty acid...