A5019065330- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Protein Hydrolysis and Bioactive Peptides
- Food Quality and Safety Studies
- Traditional Chinese Medicine Analysis
- Proteins in Food Systems
- Seed Germination and Physiology
- Phytoestrogen effects and research
- Fungal Biology and Applications
- GABA and Rice Research
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- Genetically Modified Organisms Research
- Polyamine Metabolism and Applications
- Food Allergy and Anaphylaxis Research
- Probiotics and Fermented Foods
- Biochemical effects in animals
- Plant-Microbe Interactions and Immunity
- Infant Nutrition and Health
- Plant-based Medicinal Research
- Microbial Metabolism and Applications
- Growth and nutrition in plants
- Metabolism and Genetic Disorders
- Plant Reproductive Biology
- Phytase and its Applications
State Key Laboratory of Food Science and Technology
2010-2024
Nanchang University
2010-2024
Shanghai Agrobiological Gene Center
2021
Northeast Forestry University
2017
Shaanxi University of Science and Technology
2012
Wuhan Polytechnic University
2012
Jinzhou Medical University
2008-2010
Hangzhou Quality and Technical Supervision and Testing Institute
2010
Shenzhen Center for Disease Control and Prevention
2004
Here, we describe a protocol based on semi-site-specific primer PCR (3SP-PCR) to access unknown flanking DNA sequences. We specify the guidelines for designing primers 3SP-PCR. also experimental procedures 3SP-PCR, along with product purification and subsequent sequencing analysis. For complete details use execution of this protocol, please refer Wei et al.1
Genome walking is a method used to retrieve unknown flanking DNA. Here, we reported wristwatch (WW) PCR, an efficient genome technique mediated by WW primers (WWPs). WWPs feature 5'- and 3'-overlap heterologous interval. Therefore, wristwatch-like structure can be formed between under relatively low temperatures. Each WW-PCR set composed of three nested (primary, secondary, tertiary) PCRs individually performed WWPs. The WWP arbitrarily annealed somewhere on the in one low-stringency cycle...
Here we describe a protocol for wristwatch PCR, an approach based on wristwatch-like structure formed between walking primers to obtain unknown flanks. We specify the criteria designing and gene-specific primers. detail how set primer permutations personalized outcomes improve efficiency. experimental procedures isolating DNA of interest using three rounds nested PCR as well subsequent steps purification, cloning, sequencing. For complete details use execution this protocol, please refer...
Abstract Various PCR-based genome-walking methods have been developed to acquire unknown flanking DNA sequences. However, the specificity and efficacy levels, operational processes, of available are unsatisfactory. This work proposes a novel walking approach, termed differential annealing-mediated racket PCR (DAR-PCR). The key DAR-PCR is use primer-mediated intra-strand annealing (ISA). An ISA primer consists 5’ root homologous known sequence heterologous 3’ bud. In single low-stringency...
There is a recent trend of using lactic acid bacteria for the production gamma-aminobutyric (GABA). This study described method that combines fermentation and self-buffered whole-cell catalysis efficient GABA Levilactobacillus brevis CD0817. Upon completion fermentation, cells were recovered to conduct by which substrate L-glutamic was catalytically decarboxylated GABA. itself maintained acidity essential decarboxylation. To maximize ability, effects cell culture method, temperature, time,...
Gamma-aminobutyric acid (GABA) has positive effects on many physiological processes. Lactic bacterial production of GABA is a future trend. This study aimed to produce sodium-ion-free fermentation process for Levilactobacillus brevis CD0817. In this fermentation, both the seed and media used L-glutamic instead monosodium L-glutamate as substrate. We optimized key factors influencing formation, adopting Erlenmeyer flask fermentation. The values glucose, yeast extract, Tween 80, manganese ion,...
A polyphenol oxidase was isolated from Agaricus bisporus using ammonium sulfate precipitation, combined with DEAE–Sepharose Fast Flow chromatography and Phenyl Sepharose CL-4B chromatography. The molecular weight of the purified determined to be about 43 kDa N-terminal sequence Ala-Thr-Asn-Ser-Gly-Thr-Leu-Ile-Ile-Phe by Edman degradation. optimum temperature pH for activity 20°C a 6.5–7.0. Moreover, it stable at 30 40°C over 60 min pre-incubation time period. Km Vmax values this catechol...
Enzymatic cross-linking is frequently used in bio-processing of dairy products since it could change the physiochemical and functional characterization. In our study, bovine α-lactalbumin was cross-linked by polyphenol oxidase from Agaricus bisporus changes structure, digestibility allergenicity were explored after cross-linking, structural alterations polymers analyzed circular dichroism spectroscopy, ultraviolet absorption spectroscopy fluorescence spectroscopy. The evaluated simulated...
Strawberry (Fragaria × ananassa Duch.) is a kind of fruit with great economic importance and widely cultivated in the world. From 2019 to 2020, serious crown rot disease was sporadically observed several strawberry cultivars including 'Zhang Ji', 'Hong Yan' 'Yue Xiu' Shanghai, China. Initially, water-soaked appeared inner tissue crown, then progressed into browning hollowing symptoms accompanied yellow discolorations young leaves. To isolate identify causal agent, small pieces taken from ten...