Phytopathogenic Sclerotinia minor Jagger causes lettuce drop, a destructive soil-borne disease. As potential biocontrol agents for this disease, 2 of 31 bacterial strains isolated from soil samples fields containing S. were identified using in vitro antagonistic assays against Jagger. Bioactivity experiments showed that Bac20 had higher inhibitory activity than Bac45. Based on 16S rRNA sequences and phylogenetic analysis combination gyrA, rpoB, purH, polC, groEL, Bac45 as Bacillus velezensis...

Curvularia lunata is an important maize foliar fungal pathogen that distributes widely in growing area China. Genome sequencing of the will provide information for globally understanding its virulence mechanism. We report genome sequences a highly virulent C. strain. Phylogenomic analysis indicates was evolved from Bipolaris maydis (Cochliobolus heterostrophus). The strain has high potential to evolve into other pathogenic stains based on analyses transposases and repeat-induced point...

In August 2022, melon (Cucumis melo), cucumber sativus) and luffa (Luffa aegyptiaca) plants showed virus-like symptoms characteristic of geminiviruses (yellowish green, mosaic patterns severe curling leaves, short internodes, stunting) in 10 greenhouses Fengxian district 20 greenhoues Jiading Shanghai, China. Fifty symptomatic leaf samples were randomly collected: 28 from melon, 17 cucumber, 5 luffa. To investigate the etiology observed disease, total DNA RNA was extracted via a extraction...

Curvularia lunata is an important pathogen causing leaf spot in maize. Significant pathogenic variation has been found C. lunata. To better understand the mechanism of this phenomenon, we consecutively put selective pressures resistant maize population on strain WS18 (low virulence) artificially. As a result, virulence was significantly enhanced. Using 2DE, 12 up-regulated and four down-regulated proteins were identified virulence-increased compared to WS18. Our analysis revealed that...

Gray leaf spot is a devastating disease caused by Stemphylium lycopersici that threatens tomato-growing areas worldwide. Typically, many pathogenesis-related and unrelated secreted proteins can be predicted in genomes using bioinformatics computer-based prediction algorithms, which help to elucidate the molecular mechanisms of pathogen-plant interactions. S. lycopersici-secreted were from 8997 set internet-based programs, including SignalP v4.1 TMHMM v2.0, big-PI Fungal Predictor, ProtComp...

We investigated the properties of platelet-activating factor acetylhydrolase (PAF-AH) derived from Trichoderma harzianum. The enzyme, comprised 572 amino acids, shares high homology with PAF-AH proteins T. koningii and other microbial species. optimum enzymatic activity occurred at pH 6 in absence Ca2+ it localized cytoplasm, we observed upregulation expression response to carbon starvation strong heat shock. Furthermore, knockout transformant growth more slowly than wild type cells...

The spontaneous combustion of residual coal in compound goaf formed by shallow and close seam mining is one the difficulties mine fire prevention control. In order to explore mechanism 22304 working face Huojitu minefield Daliuta mine, causes are studied comprehensively applying methods experiment, numerical calculation, field measurement, limit parameters obtained, risk faces divided. results show that critical oxygen concentration 8%, width inlet airside return oxidation temperature rise...

Xylanolytic enzymes are widely used in processing industries, e.g., pulp and paper, food, livestock feeds, textile. Furthermore, certain xylanotic have demonstrated the capability to improve resistance immunity of plants. Screening high-yield microbial xylanolytic enzyme producers is significant for improving large-scale cost-effective production. This study provided new evidence high-level production by a novel fungus, designated Leptosphaerulina chartarum SJTU59. Under laboratory...

Abstract Background Corynespora cassiicola , as a necrotrophic phytopathogenic ascomycetous fungus, can infect hundreds of species plants and rarely causes human diseases. This pathogen infects cucumber target spot, which has recently caused large yield losses in China. Genome sequence spore germination-associated transcriptome analysis will contribute to the understanding molecular mechanism pathogenicity germination C. . Results First, we reported draft genome sequences cucumber-sampled...

Interactions of pathogen infection, host plant resistance, and fungal communities are poorly understood. Although the use resistant watermelon cultivars is an effective control measure wilt disease, may also have significant effects on development soil-borne complexes. We characterized root rhizosphere associated with healthy diseased watermelons three different susceptibilities toward disease by paired-end Illumina MiSeq sequencing. Thirty plants including highly wilt-resistant, moderately...

Journal Article Rapid and sensitive detection of Curvularia lunata associated with maize leaf spot based on its Clg2p gene using semi‐nested PCR Get access J.M. Hou, Hou Institute Plant Pathology Applied Microbiology Heilongjiang Bayi Agricultural University Daqing China Search for other works by this author on: Oxford Academic Google Scholar B.C. Ma, Ma Y.H. Zuo, Zuo L.L. Guo, Guo S.G. Gao, Gao School Agriculture Biology Shanghai Jiaotong Key Laboratory Urban (South) Ministry Y.Y. Wang,...

This paper is the first report of impact on bacterial community in midgut Asian corn borer (Ostrinia furnacalis) by chitinase from transgenic Trichoderma strain. In this study, we detected a change fourth instar larvae using culture-independent method. Results suggested that Proteobacteria and Firmicutes were most highly represented phyla, being present all communities. The observed species richness was simple, ranging four to five 16S rRNA clone libraries. When fermentation liquids as...

The chit42 gene cloned from Metarhizium anisopliae lacks chitin-binding domain (chBD), which plays important roles in binding insoluble chitin. Five kinds of hybrid chitinase Trichoderma transformants were constructed this study, where the was fused to chBDs derived plant, bacterial, and insect sources. transformant Mc4 harboring bitter melon (Momordica charantia) displayed highest activity among all chBDs. activities Mc4, Mchit3, wild-type strain T30 44.94, 32.48, 12.38 U/mL, respectively....

Abstract Curvularia lunata is a widespread fungal pathogen infecting maize leaves. In this study, we developed genetic transformation system for C. by using the plasmid (pCPXHY1Egfp) with both enhanced green fluorescent protein gene egfp and hygromycin resistance hph. GFP rendered hyphae of transformants in culture. Most that showed constitutively expressing EGFP were comparable to wild-type strain morphological features. PCR Southern blot analyses hph further confirmed was integrated into...

Curvularia leaf spot, caused mainly by lunata, is a widespread plant disease in China. In the recent years, directional host selection pathogen, which likely results virulence differentiation pathogens, widely reported. Among hallmarks potentially associated to pathogen variation virulence, superoxide dismutase gene Sod has been found be closely related enhancement of virulence. present study, full-length was obtained via Blastn alignment against GenBank and whole genome C. lunata. order...

Strawberry (Fragaria × ananassa Duch.) is a kind of fruit with great economic importance and widely cultivated in the world. From 2019 to 2020, serious crown rot disease was sporadically observed several strawberry cultivars including 'Zhang Ji', 'Hong Yan' 'Yue Xiu' Shanghai, China. Initially, water-soaked appeared inner tissue crown, then progressed into browning hollowing symptoms accompanied yellow discolorations young leaves. To isolate identify causal agent, small pieces taken from ten...

A set of 78 Rhizoctonia solani isolates from Shanghai, China, were examined for their sensitivity in vitro to four demethylase inhibitor (DMI) fungicides, epoxiconazole, difenoconazole, hexaconazole, and tebuconazole, by the mycelial growth rate method. The very sensitive DMI fungicides. ranges means EC50 values 0.004-0.045 0.014 ± 0.007 μg ml-1 0-0.103 0.010 0.016 0.001-0.078 0.011 0.013 0.001-0.210 0.034 0.035 respectively. frequency distribution showed a normal curve epoxiconazole an...

Abstract Salmonella , Shigella and Escherichia coli O157:H7 contaminate similar types of food cause foodborne disease. The objective this study was to develop a selective enrichment broth for simultaneous Enteritidis, E . O157:H7, flexneri (SES) using Bile salt No. 3, potassium tellurite, novobiocin sodium, lithium chloride as agents. Developed SES not only enriched both the target pathogens high 9 log10 CFU/ml after 24 hr incubation at 37 °C with 10–100 inoculation concentration, but also...