A5019307239- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Birth, Development, and Health
- Nematode management and characterization studies
- Reproductive Biology and Fertility
- RNA modifications and cancer
- Ovarian function and disorders
- Fungal and yeast genetics research
- Liver Disease Diagnosis and Treatment
- Genomics and Phylogenetic Studies
- Ovarian cancer diagnosis and treatment
- Insect Resistance and Genetics
- Genetic Mapping and Diversity in Plants and Animals
- Bacterial biofilms and quorum sensing
- Plant Stress Responses and Tolerance
- Fungal Biology and Applications
- Assisted Reproductive Technology and Twin Pregnancy
- Mycotoxins in Agriculture and Food
- Plant tissue culture and regeneration
- Plant Disease Resistance and Genetics
- Plant chemical constituents analysis
- Dietary Effects on Health
- Insect symbiosis and bacterial influences
- Insect Pest Control Strategies
- Insect and Pesticide Research
Obstetrics and Gynecology Hospital of Fudan University
2023
Shanghai Jiao Tong University
2013-2021
International Peace Maternity & Child Health Hospital
2020-2021
Shanghai Veterinary Research Institute
2017
Shanghai Normal University
2016
Tajen University
2010
Abstract Fusarium stalk rot (FSR) caused by graminearum (FG) significantly affects the productivity of maize grain crops. Application agrochemicals to control disease is harmful environment. In this regard, use biocontrol agent (BCA) an alternative agrochemicals. Although Trichoderma species are known as BCA, selection host-pathogen specific essential for successful field application. Hence, we screened a total 100 isolates against FG, selected harzianum (CCTCC-RW0024) greenhouse experiments...
Due to its efficient broad-spectrum antimicrobial activity, Trichoderma has been established as an internationally recognized biocontrol fungus. In this study, we found and identified a novel strain of asperellum, named GDFS1009. The mycelium T. asperellum GDFS1009 exhibits high growth rate, sporulation capacity, strong inhibitory effects against pathogens that cause cucumber fusarium wilt corn stalk rot. secretes chitinase, glucanase, protease, which can degrade the cell walls fungi...
Abstract Trichoderma harzianum is well known to exhibit induced systemic resistance (ISR) Curvularia leaf spot. We previously reported that a C6 zinc finger protein ( Thc6 ) responsible for major contribution the ISR disease, but types of effectors and signals mediated by from are unclear. In this work, we demonstrated two hydrolases, Thph1 Thph2 , T. were regulated . Furthermore, an electrophoretic mobility shift assay (EMSA) study revealed mRNA expression binding GGCTAA GGCTAAA in...
Bacillus spp. are a genus of biocontrol bacteria widely used for antibiosis, while Trichoderma fungi that abundantly explored. In this study, liquid co-cultivation these two organisms was tried firstly.Through chromatography-mass spectrometry/mass spectrometry (LC-MS/MS), it discovered with an inoculation in the ratio 1.9:1, antimicrobial effect co-cultured fermentation liquor amyloliquefaciens ACCC11060 and asperellum GDFS1009 found to be significantly higher than pure-cultivation. A raise...
The genus Trichoderma is important to humankind, with a wide range of applications in industry, agriculture, and bioremediation. Thus, quick accurate identification species paramount, since it usually the first step -based research. However, frequently becomes limitation, especially for researchers who lack taxonomic knowledge fungi. Moreover, as number studies has increased, growing unidentified sequences have been stored public databases, which made more ambiguous. In this study, we...
Sheath blight, causes by Rhizoctonia spp., threaten maize yield every year throughout the world. Trichoderma could degrade solani on mainly via competition and hyperparasitism, whereas validamycin A efficiently inhibit growth of R. disturbing energy system. By contrast, is efficient but it takes effect in a short period, while long period though time-consuming. To overcome disadvantages, asperellum GDFS1009 was used together with A. In vitro tests proved that combined pathogen-inhibiting...
Frozen and thawed embryo transfer (FET) is currently widely applied in routine assisted reproductive technology (ART) procedure. It of great necessity to assess the safety FET investigate long-term effect including glucose metabolism on FET-conceived offspring. The mouse model a highly efficient method figure out relationship between process offspring health. In this study, we obtained natural conception (NC), vitro fertilization (IVF), FET. Glucose insulin tolerance test (GTT/ITT) were...
Abstract Trichoderma harzianum is a plant‐beneficial fungus that secretes small cysteine‐rich proteins induce plant defense responses; however, the molecular mechanism involved in this induction largely unknown. Here, we report class II hydrophobin ThHyd1 acts as an elicitor of induced systemic resistance (ISR) plants. Immunogold labeling and immunofluorescence revealed localized on maize ( Zea mays ) root cell plasma membranes. To identify host protein interactors Hyd1, screened B73 cDNA...
To investigate the biodiversity of Trichoderma (Hypocreaceae) and their relation to sediment physical chemical properties, we collected a total 491 samples from coastal wetlands (tidal flat wetland) in Southeast China. Further, applied two types molecular approaches such as culture dependent independent methods for identification spp. A 254 isolates were obtained identified 13 species T. aureoviride, asperellum, harzianum, atroviride, koningiopsis, longibrachiatum, koningii. tawa,...
The ortholog of Aspergillus nidulans VelB, which is known as ClVelB, was studied to gain a broader insight into the functions velvet protein in Curvularia lunata. With expected common and specific deletion clvelB results similar though not identical phenotypes. pathogenicity assays revealed that ΔClVelB impaired colonizing host tissue, corresponds finding ClVelB controls production conidia methyl 5-(hydroxymethyl) furan-2-carboxylate toxin C. However, led increase aerial hyphae melanin...
Mutant T66 was isolated from 450 mutants (constructed with Agrobacterium tumefaciens ‐mediated transformation method) of Trichoderma harzianum . Maize seeds coated were more susceptible to Curvularia lunata when compared those wild‐type (WT) strain. The disease index maize treated and WT 62.5 42.1%, respectively. Further research showed T‐DNA has inserted into the ORF one gene, which resulted in functional difference between T66. gene cloned named Thc 6, encodes a novel 327 amino acid...
Amylin was found to regulate glucose and lipid metabolism by acting on the arcuate nucleus of hypothalamus (ARC). Maternal high-fat diet (HFD) induces sex-specific metabolic diseases mediated ARC in offspring. This study performed explore 1) effect maternal HFD-induced alterations amylin differentiation hypothalamic neurons disorders male offspring 2) specific molecular mechanism underlying regulation its receptor response HFD. HFD gestational hyper-amylin mice models were established role...
We investigated the properties of platelet-activating factor acetylhydrolase (PAF-AH) derived from Trichoderma harzianum. The enzyme, comprised 572 amino acids, shares high homology with PAF-AH proteins T. koningii and other microbial species. optimum enzymatic activity occurred at pH 6 in absence Ca2+ it localized cytoplasm, we observed upregulation expression response to carbon starvation strong heat shock. Furthermore, knockout transformant growth more slowly than wild type cells...
Abstract Cochliobolus heterostrophus is the causal pathogen of southern corn leaf blight ( SCLB ). There are three known races: race O, C and T. To determine which . races comprise field population in China to assess diversity these strains terms virulence, 200 isolates from diseased plants were collected nine provinces/municipalities. All that is, no T or found. Sixty O sporulated well chosen for further analysis. Virulence was measured using integral optical density IOD ) lesions on four...
Trichoderma atroviride SG3403 showed high biocontrol activity against southern corn leaf blight (SCLB; pathogen: Cochliobolus heterostrophus). T. could cause death of C. heterostrophus race O hypha on plates. Spraying conidia suspension over maize seedling leaves protected the from SCLB infection. Biocontrol effect lasted for 30 days in field. strain was able to induce resistance response pathogen In treated with SG3403, enzyme activities phenylalanine ammonia lyase (PAL) and superoxide...
Platelet-activating factor acetylhydrolase (PAF-AH) derived from Trichoderma harzianum was upregulated by the interaction of T. with maize roots or foliar pathogen Curvularia lunata. PAF-AH associated chitinase and cellulase expressions, but especially chitinase, because its activity in KO40 transformant (PAF-AH disruption transformant) lower, compared wild-type strain T28. The result demonstrated that colonization induced systemic protection leaves inoculated C. Such expression inducible...
The number of children born after assisted reproductive technology (ART) is accumulating rapidly, and the health problems are extensively concerned. This study aims to evaluate whether ART procedures alter behaviours in male offspring. Mouse models were utilized establish three groups offspring conceived by natural conception (NC), vitro fertilization embryo transfer (IVF-ET), frozen-thawed (IVF-FET), respectively. A battery behaviour experiments for evaluating anxiety depression levels,...
Abstract Objective To study the effect of aging on ovarian circadian rhythm. Design Human and animal study. Setting University hospital research laboratory. Patients/animals granulosa cells were obtained by follicular aspiration from women undergoing in vitro fertilization (IVF), liver tissues female C57BL/6 mice. Intervention(s) None. Main outcome measure(s) Expression genes young older human rhythm ovaries livers Result(s) All examined clock showed a downward trend expression with aging,...