A5100377530- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Neuroscience and Neural Engineering
- Metabolomics and Mass Spectrometry Studies
- Tuberculosis Research and Epidemiology
- Microbial infections and disease research
- Pluripotent Stem Cells Research
- Microbial Community Ecology and Physiology
- Innovative Microfluidic and Catalytic Techniques Innovation
- Plant Virus Research Studies
- Extracellular vesicles in disease
- Viral Infections and Immunology Research
- Genomics and Phylogenetic Studies
Southern University of Science and Technology
2023
Southern Medical University
2022
Hubei University of Chinese Medicine
2022
Tengzhou Central People's Hospital
2020
Zhejiang University
2018
The path toward field-effect transistor (FET) application from laboratory to clinic has delivered a compelling push in the biomedical domain, yet ultrasensitive and timely pathogen identification without PCR remains long-lasting challenge. Herein, we create generic check station termed "CRISPR-FET", first incorporating CRISPR/Cas13a system within FET modality, for accelerated unamplified detection of viral RNA. Unlike conventional FETs bearing target-specific receptors, this sensor holds...
Graphical Abstract Schematic of Mycoplasma hominis nucleic acid detection based on the CRISPR-Cas12a system. Clinical samples from cervical or urethral swabs were collected and incubated with lysis buffer to release (10 min). Extracted DNA (1 μl) is subjected RPA reaction specific primers at 37°C. After 20 min, product was for cleavage. The collateral nuclease activity Cas12a (250 nM) proteins activated upon binding crRNA (crRNA3, 62.5 product; thus, cut quenched fluorescent ssDNA reporter...
Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis, which infects more than 23% of the world's population. With emergence drug-resistant TB (DR-TB) and latent infection (LTBI), rapid diagnosis DR-TB LTBI has become a challenge for prevention control TB. Herein, we highlight these challenges discuss emerging clustered regularly interspaced short palindromic repeats (CRISPR)-based diagnostics in detection. Currently, clinical M. tuberculosis mainly depends on...
Basonuclin (BNC1) is expressed primarily in proliferative keratinocytes and gametogenic cells. However, its role spermatogenesis not clear. Previously we discovered a heterozygous BNC1 truncating mutation premature ovarian insufficiency pedigree. In this study, found that male mice carrying the exhibited sub-fertility. Genome-wide expression profiling direct binding studies(ChIP-seq) with mouse testis identified several specific gene promoters targeted by including Klhl10, Tex14, Spatc1....
Exosomes-derived miRNA-21 (miR-21) is intimately correlated with the lung cancer and can be used as a biomarker for early diagnosis of cancer. Due to low abundance, high homology complex matrix miR-21 in exosomes, accurate determination exosomal remains challenging. Combination CRISPR technology biosensors improves detection specificity sensitivity. This article reports CRISPR/Cas12a-based fluorescence bioassay derived from by coupling state-of-the-art technologies including strand...