A5024291281- RNA modifications and cancer
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Cancer-related gene regulation
- Bacteriophages and microbial interactions
- Genomics and Chromatin Dynamics
University of California, Berkeley
2024-2025
Tufts University
2017
Genome regulation relies on complex and dynamic interactions between DNA proteins. Recently, powerful methods have emerged that leverage third-generation sequencing to map protein-DNA genome-wide. For example, Directed Methylation with Long-read (DiMeLo-seq) enables mapping of along long, single chromatin fibers, including in highly repetitive genomic regions. However, DiMeLo-seq involves lossy centrifugation-based wash steps limit its applicability many sample types. To address this, we...
Abstract We recently developed Di rected Me thylation with Lo ng-read seq uencing (DiMeLo-seq) to map protein-DNA interactions genome wide. DiMeLo-seq is capable of mapping multiple interaction sites on single DNA molecules, profiling protein binding in the context endogenous methylation, identifying haplotype specific interactions, and repetitive regions that are difficult study short-read methods. With DiMeLo-seq, adenines vicinity a interest methylated situ by tethering Hia5...