A5059143372- Protein Structure and Dynamics
- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- Radioactive contamination and transfer
- Mitochondrial Function and Pathology
- Mass Spectrometry Techniques and Applications
- Neuroendocrine regulation and behavior
- Evolutionary Psychology and Human Behavior
- Protein Kinase Regulation and GTPase Signaling
- Neuroscience and Neuropharmacology Research
- Cellular transport and secretion
- Endoplasmic Reticulum Stress and Disease
- RNA and protein synthesis mechanisms
- Hypothalamic control of reproductive hormones
Stanford University
2025
Brown University
2021-2023
John Brown University
2022
Carleton College
2018
Genome regulation relies on complex and dynamic interactions between DNA proteins. Recently, powerful methods have emerged that leverage third-generation sequencing to map protein-DNA genome-wide. For example, Directed Methylation with Long-read (DiMeLo-seq) enables mapping of along long, single chromatin fibers, including in highly repetitive genomic regions. However, DiMeLo-seq involves lossy centrifugation-based wash steps limit its applicability many sample types. To address this, we...
In enterobacteria such as Escherichia coli, the general stress response is mediated by σs, stationary phase dissociable promoter specificity subunit of RNA polymerase. σs degraded ClpXP during active growth in a process dependent on RssB adaptor, which thought to be stimulated phosphorylation conserved aspartate its N-terminal receiver domain. Here we present crystal structure full-length bound beryllofluoride phosphomimic. Compared IraD anti-adaptor, our new with reveals conformational...
In the model organism Escherichia coli and related species, general stress response relies on tight regulation of intracellular levels promoter specificity subunit RpoS. RpoS turnover is exclusively dependent RssB, a two-domain regulator that functions as an adaptor delivers to ClpXP for proteolysis. Here, we report crystal structures receiver domain RssB both in its unphosphorylated form bound phosphomimic BeF3 - . Surprisingly, find only modest differences between these two structures,...
SUMMARY In γ-proteobacteria such as Escherichia coli , the general stress response is mediated by σ s stationary phase dissociable promoter specificity subunit of RNA polymerase. degraded ClpXP during active growth in a process dependent on RssB adaptor, which acts catalytically and thought to be stimulated phosphorylation conserved aspartate its N-terminal receiver domain. Here we present crystal structure full-length bound beryllofluoride phosphomimic. Compared inhibited IraD...
ABSTRACT In the model organism Escherichia coli and related species, general stress response relies on tight regulation of intracellular levels promoter specificity subunit RpoS. RpoS turnover is exclusively dependent RssB, a two-domain regulator that functions as an adaptor delivers to ClpXP for proteolysis. Here we report crystal structures receiver domain RssB both in its unphosphorylated form bound phosphomimic BeF 3 − . Surprisingly, find only modest differences between these two...