Ali Rezvan
A5007513526- Immune Cell Function and Interaction
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Cancer, Hypoxia, and Metabolism
- Vaccine Coverage and Hesitancy
- Single-cell and spatial transcriptomics
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- CRISPR and Genetic Engineering
- Cancer Genomics and Diagnostics
- Nanowire Synthesis and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Nanofabrication and Lithography Techniques
- Influenza Virus Research Studies
- Biosimilars and Bioanalytical Methods
- RNA Interference and Gene Delivery
- Digital Holography and Microscopy
- Respiratory viral infections research
- interferon and immune responses
- COVID-19 epidemiological studies
- Graphene and Nanomaterials Applications
- Virus-based gene therapy research
- Advanced Fluorescence Microscopy Techniques
University of Houston
2019-2024
BD Biosciences (United States)
2024
Allogene Therapeutics (United States)
2022
Bristol-Myers Squibb (Germany)
2022
Karus Therapeutics (United Kingdom)
2022
University of Tehran
2019
Despite remarkable progress in the development and authorization of vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), there is a need to validate vaccine platforms for broader application. The current intramuscular are designed elicit systemic immunity without conferring mucosal nasal compartment, which first barrier that SARS-CoV-2 virus breaches before dissemination lung. We report an intranasal subunit uses lyophilized spike protein liposomal STING agonist as...
The in vivo persistence of adoptively transferred T cells is predictive antitumor response. Identifying functional properties infused that lead to and tumor eradication has remained elusive. We profiled CD19-specific chimeric antigen receptor (CAR) as the infusion products used treat large B cell lymphomas using high-throughput single-cell technologies based on time-lapse imaging microscopy nanowell grids (TIMING), which integrates killing, cytokine secretion, transcriptional profiling. Our...
Multiple factors in the design of a chimeric antigen receptor (CAR) influence CAR T-cell activity, with costimulatory signals being key component. Yet, impact domains on downstream signaling and subsequent functionality CAR-engineered natural killer (NK) cells remains largely unexplored. Here, we evaluated various CAR-NK cell using CD70-targeting CAR. We found that CD28, molecule not inherently present mature NK cells, significantly enhanced antitumor efficacy long-term cytotoxicity both...
Abstract Chimeric antigen receptor (CAR) T cell show promise in cancer treatments, but their mechanism of action is not well understood. Decoding the mechanisms used by individual cells can help improve efficacy while also identifying failure leading to tumor escape. Here, we a suite assays including dynamic single-cell imaging cell-cell interactions, fluorescent reporters directly track cytotoxin activity cells, and scRNA-seq on patient infusion products investigate cytotoxic CAR killing...
Abstract Immunization programs against SARS-CoV-2 with commercial intramuscular vaccines prevent disease but are less efficient in preventing infections. Mucosal can provide improved protection transmission, ideally for different variants of concern (VOCs) and related sarbecoviruses. Here, we report a multi-antigen, intranasal vaccine, NanoSTING-SN (NanoSTING-Spike-Nucleocapsid), eliminates virus replication both the lungs nostrils upon challenge pathogenic Delta VOC. We further demonstrate...
Extracellular vesicles (EVs) regulate the tumor microenvironment by facilitating transport of biomolecules. Despite extensive investigation, heterogeneity in EV secretion among cancer cells and mechanisms that support are not well characterized. We developed an integrated method to identify individual with differences performed linked single-cell RNA-sequencing on cloned single from metastatic breast cells. Differential gene expression analyses identified a four-gene signature secretion:...
Abstract A safe and durable vaccine is urgently needed to tackle the COVID19 pandemic that has infected >15 million people caused >620,000 deaths worldwide. As with other respiratory pathogens, nasal compartment first barrier needs be breached by SARS-CoV-2 virus before dissemination lung. Despite progress at remarkable speed, current intramuscular vaccines are designed elicit systemic immunity without conferring mucosal immunity. We report development of an intranasal subunit contains...
Time lapse microscopy is essential for quantifying the dynamics of cells, subcellular organelles and biomolecules. Biologists use different fluorescent tags to label track structures biomolecules within cells. However, not all them are compatible with time imaging, labeling itself can perturb cells in undesirable ways. We hypothesized that phase image has requisite information identify nuclei By utilizing both traditional blob detection generate binary mask labels from stained channel images...
An increasing number of new strategies for skin tissue engineering have been developed with the potential to mimic biological properties native a high degree complexity, flexibility, and reproducibility. In this study, decellularized (DT) was prepared from bovine heart by using chemical treatments. However, mechanical DT constructs were poorer than extra cellular matrix tissue. To overcome challenge, hybrid scaffolds graphene oxide (GO) effects GO concentration on morphology, pore size,...
Abstract Full-length TCR and BCR analysis with single-cell multiomic assays provides an understanding of immune responses, offering insights into the complete receptor structure functionality that is crucial for accurately assessing diversity specificity repertoires. Studying mouse cell heterogeneity can be achieved by simultaneous mRNA surface protein profiling. However, when coupled full-length analysis, this integrated approach a more comprehensive responses cellular function in healthy...
<div>Abstract<p>Multiple factors in the design of a chimeric antigen receptor (CAR) influence CAR T-cell activity, with costimulatory signals being key component. Yet, impact domains on downstream signaling and subsequent functionality CAR-engineered natural killer (NK) cells remains largely unexplored. Here, we evaluated various CAR-NK cell using CD70-targeting CAR. We found that CD28, molecule not inherently present mature NK cells, significantly enhanced antitumor efficacy...
<p>In vitro characterization of the CAR27 constructs.</p>
<p>CAR27-28ζ NK cells show polyfunctionality.</p>
<p>Expression of CD70 at the protein and mRNA levels in different tumor types.</p>
<p>In vivo antitumor activity of CAR27-expressing NK cells.</p>
<p>CAR27-28ζ NK cell serial killing against Raji cells.</p>
<p>In a PDX mouse model of metastatic breast cancer, CAR27-28ζ NK cells lead to reduction in tumor size and increased cell infiltration the lungs.</p>
<p>CAR27-ζ NK cell serial killing against Raji cells.</p>