A5057011524- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- Mosquito-borne diseases and control
- Synthesis and Biological Evaluation
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- CAR-T cell therapy research
- Phagocytosis and Immune Regulation
- T-cell and B-cell Immunology
- interferon and immune responses
- Cancer Research and Treatments
- COVID-19 Clinical Research Studies
- Stress Responses and Cortisol
- Pancreatic and Hepatic Oncology Research
- Viral Infections and Vectors
- Lung Cancer Research Studies
- Tryptophan and brain disorders
- Neurogenesis and neuroplasticity mechanisms
- Acute Myeloid Leukemia Research
- Cancer Cells and Metastasis
- Ultrasound and Hyperthermia Applications
- Respiratory viral infections research
- PARP inhibition in cancer therapy
- HIV Research and Treatment
The University of Texas MD Anderson Cancer Center
1993-2024
Ben-Gurion University of the Negev
2017-2022
National Brain Research Centre
2012-2015
ABSTRACT MicroRNAs (miRNAs) are single-stranded small RNA molecules that regulate various cellular processes. miRNA 155 (miR-155) regulates aspects of innate and adaptive immune responses plays a key role in viral infections the resulting neuroinflammation. The present study evaluated involvement miR-155 modulating Japanese encephalitis virus (JEV)-induced We observed expression was upregulated during JEV infection mouse primary microglia, BV-2 microglia cell line, both human brains. In...
Multiple factors in the design of a chimeric antigen receptor (CAR) influence CAR T-cell activity, with costimulatory signals being key component. Yet, impact domains on downstream signaling and subsequent functionality CAR-engineered natural killer (NK) cells remains largely unexplored. Here, we evaluated various CAR-NK cell using CD70-targeting CAR. We found that CD28, molecule not inherently present mature NK cells, significantly enhanced antitumor efficacy long-term cytotoxicity both...
Japanese encephalitis virus (JEV), a single-stranded RNA (ssRNA) virus, is the leading cause of in Asia. Microglial activation one key events JEV-induced neuroinflammation. Although various microRNAs (miRNAs) has been shown to regulate microglia during pathological conditions including neuroviral infections, till date, involvement miRNAs JEV infection not evaluated. Hence, we sought evaluate possible role mediating activation. Initial screening revealed significant up-regulation miR-29b...
Coronavirus disease 2019 is an infectious caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which enters host cells through the cell surface proteins ACE2 and TMPRSS2. Using a variety of normal malignant models tissues from aerodigestive tracts, we investigated expression regulation We find that restricted to select population epithelial cells. Notably, infection with SARS-CoV-2 in cancer lines, bronchial organoids, patient nasal epithelium induces metabolic...
Myelodysplastic syndrome and acute myeloid leukemia (AML) belong to a continuous disease spectrum of malignancies with poor prognosis in the relapsed/refractory setting necessitating novel therapies. Natural killer (NK) cells from patients display global dysfunction impaired killing capacity, altered metabolism, an exhausted phenotype at single-cell transcriptomic proteomic levels. In this study, we identified that was mediated through cross-talk between NK blasts cell-cell contact. cell...
COVID-19 is an infectious disease caused by SARS-CoV-2, which enters host cells via the cell surface proteins ACE2 and TMPRSS2. Using a variety of normal malignant models tissues from aerodigestive respiratory tracts, we investigated expression regulation
Lurbinectedin recently received FDA accelerated approval as a second line treatment option for metastatic small cell lung cancer (SCLC). However, there are currently no established biomarkers to predict SCLC sensitivity or resistance lurbinectedin preclinical studies guide rational combinations.Drug was assayed in proliferation assays and xenograft models. Baseline proteomic profiling performed by reverse-phase protein array. Lurbinectedin-induced changes intracellular signaling pathways...
Abstract mAb-based blocking of the immune checkpoints involving CTLA4-B7 and PD1-PDL1 inhibitory axes enhance T-cell–based adaptive responses in patients with cancer. We show here that antitumor by natural killer (NK) cells can be enhanced a checkpoint-blocking mAb, 14-25-9, which we developed against proliferating cell nuclear antigen (PCNA). PCNA is expressed on surface cancer acts as an ligand for NK-cell receptor, NKp44-isoform1. tested cytoplasmic- membrane-associated FACS-...
Proliferating Cell Nuclear Antigen (PCNA) is considered as a hub protein and key regulator of DNA replication, repair, cell cycle control apoptosis. PCNA over-expressed in many cancer types PCNA-overexpression correlated with virulence. Membrane-associated ligand for the NKp44 (NCR2) innate immune receptor. The purpose this study was to characterize binding site within NKp44. We have identified NKp44-derived linear peptide (pep8), which can specifically interact partly block NKp44-PCNA...
Abstract IL-2 is the master-regulator cytokine for T cell dependent responses and crucial proliferation survival of cells. However, IL-2-based treatments remained marginal, in part due to short half-life. Thus, we aimed extend half-life by flanking core with sequences derived from extensively glycosylated hinge region NCR2 receptor. We termed this modified IL-2: “S2A”. Importantly, S2A blood was extended 14-fold compared clinical grade IL-2, Proleukin. Low doses inoculation significantly...
Abstract Harnessing the immune-system to eradicate cancer is becoming a reality in recent years. Engineered immune cells, such as chimeric antigen receptor (CAR) T are facing danger of an overt life-threatening response due ON-target OFF-tumor cytotoxicity and Cytokine Release Syndrome. We therefore developed synthetic promoters for regulation gene expression under control inflammation Hypoxia-induced signals that associated with tumor microenvironment (TME). termed this methodology...
Abstract Objectives Emergence of multidrug resistance among Streptococcus pneumoniae (SP), has limited the available options used to treat infections caused by this organism. The objective study was compare role monotherapy and combination therapy with ampicillin (AMP) azithromycin (AZM) in eradicating bacterial burden down regulating lung inflammation a murine experimental pneumococcal infection model. Methods Balb/C mice were infected 10 6 CFU SP. Treatments intravenous (200 mg/kg) (50...
Despite of remarkable progress made in the head and neck cancer (HNC) therapy, survival rate this metastatic disease remain low. Tailoring appropriate therapy to patients is a major challenge highlights unmet need have good preclinical model that will predict clinical response. Hence, we developed an accurate time efficient drug screening method tumor ex vivo analysis (TEVA) system, which can patient-specific responses. In study, generated six patient derived xenografts (PDXs) were utilized...
An immune role of neural stem/progenitor cells (NSPCs) has been proposed in many recent studies; however much still remains to be elucidated. In the current investigation, we report that NSPCs possess ability convert encephalitogenic T into CD4(+)-CD25(+)-FOXP3(+) regulatory (T(reg)). Encephalitogenic and nonencephalitogenic isolated from sham Japanese encephalitis virus (JEV) infected animals were co-cultured with mouse NSPCs. Post co-culture, significant increase number T(regs) was...
The Ebola Virus (EBOV) glycoprotein (GP) sterically shields cell-membrane ligands to immune receptors such as human leukocyte antigen class-1 (HLA-I) and MHC class I polypeptide-related sequence A (MICA), thus mediating immunity evasion. It was suggested that the abundant N-glycosylation of EBOV-GP is involved in this steric shielding. We aimed characterize (i) GP sites contributing shielding, (ii) effect mutating these on subversion by EBOV-GP. two highly glycosylated domains are mucin-like...
The natural killer (NK) cell activating receptor NKp46/NCR1 plays a critical role in elimination of virus-infected and tumor cells. NCR1 gene can be transcribed into five different splice variants, but the functional importance physiological distribution NKp46 isoforms are not yet fully understood. Here, we shed light on differential expression variants viral respiratory tract infections their difference at cellular level. was most predominantly expressed cytotoxicity nasal lavage patients...