Mohsen Fathi
A5046787141- CAR-T cell therapy research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Nanowire Synthesis and Applications
- Single-cell and spatial transcriptomics
- interferon and immune responses
- Influenza Virus Research Studies
- Cancer Genomics and Diagnostics
- Cancer, Hypoxia, and Metabolism
- Enzyme Structure and Function
- Respiratory viral infections research
- RNA Research and Splicing
- Vaccine Coverage and Hesitancy
- Monoclonal and Polyclonal Antibodies Research
- Enzyme Production and Characterization
- Biosimilars and Bioanalytical Methods
- Nanofabrication and Lithography Techniques
- Mycotoxins in Agriculture and Food
- Breast Cancer Treatment Studies
- Molecular Biology Techniques and Applications
- Electrostatic Discharge in Electronics
University of Houston
2019-2024
Occidental Petroleum (United States)
2023
Despite remarkable progress in the development and authorization of vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), there is a need to validate vaccine platforms for broader application. The current intramuscular are designed elicit systemic immunity without conferring mucosal nasal compartment, which first barrier that SARS-CoV-2 virus breaches before dissemination lung. We report an intranasal subunit uses lyophilized spike protein liposomal STING agonist as...
The in vivo persistence of adoptively transferred T cells is predictive antitumor response. Identifying functional properties infused that lead to and tumor eradication has remained elusive. We profiled CD19-specific chimeric antigen receptor (CAR) as the infusion products used treat large B cell lymphomas using high-throughput single-cell technologies based on time-lapse imaging microscopy nanowell grids (TIMING), which integrates killing, cytokine secretion, transcriptional profiling. Our...
Multiple factors in the design of a chimeric antigen receptor (CAR) influence CAR T-cell activity, with costimulatory signals being key component. Yet, impact domains on downstream signaling and subsequent functionality CAR-engineered natural killer (NK) cells remains largely unexplored. Here, we evaluated various CAR-NK cell using CD70-targeting CAR. We found that CD28, molecule not inherently present mature NK cells, significantly enhanced antitumor efficacy long-term cytotoxicity both...
Significance The mesoscopic p53-rich clusters that we discover represent a new class of biological condensate, distinct from amorphous and ordered aggregates the dense liquids found with several physiologically active proteins. demonstrated two-step mechanism amyloid fibril nucleation, whereby host nucleation p53 fibrils, illustrates potency recently identified nonclassical concepts to understand intracellular processes. This finding establishes biophysical paradigm for assembly numerous...
Extracellular vesicles (EVs) mediate communication in health and disease. Conventional assays are limited profiling EVs secreted from large populations of cells cannot map EV secretion onto individual their functional profiles. We developed a high-throughput single-cell technique that enabled the mapping dynamics secretion. By utilizing breast cancer cell lines, we established is heterogeneous at level non-metastatic can secrete specific subsets EVs. Single-cell RNA sequencing confirmed...
Abstract Respiratory viral infections cause morbidity and mortality worldwide. Despite the success of vaccines, vaccination efficacy is weakened by rapid emergence variants with immunoevasive properties. The development an off-the-shelf, effective, safe therapy against respiratory thus desirable. Here, we develop NanoSTING, a nanoparticle formulation endogenous STING agonist, 2′−3′ cGAMP, to function as immune activator demonstrate its safety in mice rats. A single intranasal dose NanoSTING...
Extracellular vesicles (EVs) regulate the tumor microenvironment by facilitating transport of biomolecules. Despite extensive investigation, heterogeneity in EV secretion among cancer cells and mechanisms that support are not well characterized. We developed an integrated method to identify individual with differences performed linked single-cell RNA-sequencing on cloned single from metastatic breast cells. Differential gene expression analyses identified a four-gene signature secretion:...
Abstract A safe and durable vaccine is urgently needed to tackle the COVID19 pandemic that has infected >15 million people caused >620,000 deaths worldwide. As with other respiratory pathogens, nasal compartment first barrier needs be breached by SARS-CoV-2 virus before dissemination lung. Despite progress at remarkable speed, current intramuscular vaccines are designed elicit systemic immunity without conferring mucosal immunity. We report development of an intranasal subunit contains...
Abstract Respiratory viral infections, especially Influenza (endemic) or SARS-CoV-2 (pandemic since 2020), cause morbidity and mortality worldwide. Despite remarkable progress in the development deployment of vaccines, they are clearly impacted by rapid emergence variants. The an off-the-shelf, effective, safe, low-cost drug for prophylaxis against respiratory infections is a major unmet medical need. Here, we developed NanoSTING, liposomally encapsulated formulation endogenous STING...
Abstract The oncogenic properties of mutant p53 have been ascribed to destabilization the conformation, followed by aggregation into insoluble fibrils. Here we combine immunofluorescent 3D confocal microscopy breast cancer cells expressing Arg248Gln (R248Q) with light scattering from solutions purified protein and molecular simulations probe mechanisms that govern phase behaviors across multiple length scales, cellular molecular. We establish R248Q forms mesoscopic protein-rich clusters, an...
Understanding immune response to infections and vaccines lags understanding humoral responses. While neutralizing antibody responses wane over time, T cells are instrumental in long-term immunity. We apply machine learning time-lapse imaging microscopy nanowell grids (TIMING) study thousands of videos with specificity for SARS-CoV-2 eliminating targets bearing spike protein as a surrogate viral infection. The data on effector functions, including cytokine secretion cytotoxicity, provide the...
ABSTRACT Adoptive chimeric antigen receptor T-cell (CAR-T) therapy is transformative and approved for hematologic malignancies. It also being developed the treatment of solid tumors, autoimmune disorders, heart disease, aging. Despite unprecedented clinical outcomes, CAR-T other engineered cell therapies face a variety manufacturing safety challenges. Traditional methods, such as lentivirus transduction electroporation, result in random integration or cause significant cellular damage, which...
<title>Abstract</title> Adoptive chimeric antigen receptor T-cell (CAR-T) therapy is transformative and approved for hematologic malignancies. It also being developed the treatment of solid tumors, autoimmune disorders, heart disease, aging. Despite unprecedented clinical outcomes, CAR-T other engineered cell therapies face a variety manufacturing safety challenges. Traditional methods, such as lentivirus transduction electroporation, result in random integration or cause significant...
<h3>Background</h3> Cellular immunotherapy using chimeric antigen receptor T cell (CAR T) therapy has revolutionized treatment of patients with hematologic malignancy. Despite success, as many half leukemia experience disease progression. CAR infusion product potency is often compromised by the poor health cells recovered from and intrinsic cellular mechanisms, such activation-induced death (AICD), that limit duration anti-tumor response. To enhance overall efficacy predict potency,...
<div>Abstract<p>Multiple factors in the design of a chimeric antigen receptor (CAR) influence CAR T-cell activity, with costimulatory signals being key component. Yet, impact domains on downstream signaling and subsequent functionality CAR-engineered natural killer (NK) cells remains largely unexplored. Here, we evaluated various CAR-NK cell using CD70-targeting CAR. We found that CD28, molecule not inherently present mature NK cells, significantly enhanced antitumor efficacy...
<p>In vitro characterization of the CAR27 constructs.</p>
<p>CAR27-28ζ NK cells show polyfunctionality.</p>
<p>Expression of CD70 at the protein and mRNA levels in different tumor types.</p>
<p>In vivo antitumor activity of CAR27-expressing NK cells.</p>