A5029189632- Microfluidic and Bio-sensing Technologies
- CAR-T cell therapy research
- Microfluidic and Capillary Electrophoresis Applications
- Nanowire Synthesis and Applications
- Lattice Boltzmann Simulation Studies
- RNA Interference and Gene Delivery
- Fluid Dynamics and Mixing
- CRISPR and Genetic Engineering
- Electrical and Bioimpedance Tomography
- 3D Printing in Biomedical Research
- Electrowetting and Microfluidic Technologies
- Fluid Dynamics and Heat Transfer
- Flow Measurement and Analysis
- Aerosol Filtration and Electrostatic Precipitation
- Nanofabrication and Lithography Techniques
- Electrostatic Discharge in Electronics
UNSW Sydney
2013-2014
Abstract Intracellular delivery of functional macromolecules, such as DNA and RNA, across the cell membrane into cytosol, is a critical process in both biology medicine. Herein, we develop use microfluidic chips containing post arrays to induce vortex shedding , or μVS for poration that permits mRNA primary human T lymphocytes. We demonstrate transfection with by 996-nucleotide construct encoding enhanced green fluorescent protein (EGFP) assessed efficiencies quantifying levels EGFP...
Deterministic lateral displacement (DLD) is a microfluidic size-based particle separation or filter technology with applications in cell and enrichment. Currently, there are no cost-effective manufacturing methods for this promising technology. In fabrication paper, however, we develop simple, yet robust protocol thermoplastic DLD devices using regulatory-approved materials biocompatible methods. The final standalone device allowed volumetric flow rates of 660 μl min(-1) while reducing the...
Abstract Microfluidic vortex shedding ( µVS ) can rapidly deliver mRNA to T cells with high yield and minimal perturbation of the cell state. The mechanistic underpinning intracellular delivery remains undefined -Cas9 genome editing requires further studies. Herein, we evaluated a series devices containing splitter plates attenuate understand contribution computed force frequency on efficiency viability. We then selected design knockout expression endogenous receptor in primary human via...
ABSTRACT Adoptive chimeric antigen receptor T-cell (CAR-T) therapy is transformative and approved for hematologic malignancies. It also being developed the treatment of solid tumors, autoimmune disorders, heart disease, aging. Despite unprecedented clinical outcomes, CAR-T other engineered cell therapies face a variety manufacturing safety challenges. Traditional methods, such as lentivirus transduction electroporation, result in random integration or cause significant cellular damage, which...
<title>Abstract</title> Adoptive chimeric antigen receptor T-cell (CAR-T) therapy is transformative and approved for hematologic malignancies. It also being developed the treatment of solid tumors, autoimmune disorders, heart disease, aging. Despite unprecedented clinical outcomes, CAR-T other engineered cell therapies face a variety manufacturing safety challenges. Traditional methods, such as lentivirus transduction electroporation, result in random integration or cause significant...
<h3>Background</h3> Cellular immunotherapy using chimeric antigen receptor T cell (CAR T) therapy has revolutionized treatment of patients with hematologic malignancy. Despite success, as many half leukemia experience disease progression. CAR infusion product potency is often compromised by the poor health cells recovered from and intrinsic cellular mechanisms, such activation-induced death (AICD), that limit duration anti-tumor response. To enhance overall efficacy predict potency,...
Abstract Microfluidic vortex shedding ( μVS ) can rapidly deliver mRNA to T cells with high yield. The mechanistic underpinning of intracellular delivery remains undefined and -Cas9 genome editing requires further studies. Herein, we evaluated a series devices containing splitter plates attenuate understand the contribution computed force frequency on efficiency viability. We then selected design knockout expression endogenous cell receptor in primary human via CRISPR-Cas9 ribonucleoprotein...
Microfluidic particle separation technologies are useful for enriching rare cell populations academic and clinical purposes. In order to separate particles based on size, deterministic lateral displacement (DLD) arrays designed assuming that the flow profile between posts is parabolic or shifted (depending post geometry). The design process also assumes shape of normalized speed-invariant. work presented here shows shapes vary, in with circular triangular posts, from this assumption at...
Image analysis can play an important role in the validation and of computational studies. Computational Fluid Dynamics (CFD) compared to experiments, has advantage detailed information across domain interest. However, with image not only better be extracted from experimental results, but also numerical methods CFD benefit post processing information. In this paper, we show how may applied data understand results. More specifically, simulate transient behaviour a 9 mm bubble rising 20 wide...
Micropost arrays serve as a plaform for the next generation of diagnostic devices. These are found in microfluidic devices peripheral blood-based diagnostics and metastatic cancer management. The function performance these is determined by underlying micro-scale fluid mechanics. Typically, operate creeping regime ( Re << 1) where viscous forces fluids dominate. Recent advances manufacturing allow higher Reynolds number flows >> inertial In this work, we use computational...
Abstract Intracellular delivery of functional macromolecules, such as DNA and RNA, across the cell membrane into cytosol, is a critical process in both biology medicine. Herein, we develop use microfluidic chips containing post arrays to induce vortex shedding , or μVS for poration that permits mRNA primary human T lymphocytes. We demonstrate transfection with by 996-nucleotide construct encoding enhanced green fluorescent protein (EGFP) assessed efficiencies quantifying levels EGFP...