A5014697149- Pancreatic function and diabetes
- Cardiomyopathy and Myosin Studies
- Diabetes and associated disorders
- Cellular Mechanics and Interactions
- Renal and related cancers
- Ion Transport and Channel Regulation
- Genetic and Kidney Cyst Diseases
- Bacterial biofilms and quorum sensing
- Metabolism, Diabetes, and Cancer
- Renal Diseases and Glomerulopathies
- Adhesion, Friction, and Surface Interactions
- Muscle Physiology and Disorders
- Force Microscopy Techniques and Applications
- Antimicrobial Peptides and Activities
- Kidney Stones and Urolithiasis Treatments
- Bacterial Genetics and Biotechnology
- bioluminescence and chemiluminescence research
- Antimicrobial Resistance in Staphylococcus
Sanford Research
2017-2024
University of California, Davis
2020-2024
A.T. Still University
2021
Pancreatic islets are three-dimensional cell aggregates consisting of unique cellular composition, cell-to-cell contacts, and interactions with blood vessels. Cell aggregation is essential for islet endocrine function; however, it remains unclear how developing establish aggregation. By combining genetic animal models, imaging tools, gene expression profiling, we demonstrate that regulated by extracellular matrix signaling cell-cell adhesion. Islet cell-specific inactivation receptor...
ABSTRACT Nonmuscle myosin II (NMII) generates cytoskeletal forces that drive cell division, embryogenesis, muscle contraction and many other cellular functions. However, at present there is no method can directly measure the generated by myosins in living cells. Here, we describe a Förster resonance energy transfer (FRET)-based tension sensor detect myosin-associated force along filamentous actin network. Fluorescence lifetime imaging microscopy (FLIM)-FRET measurements indicate NMII isoform...
Actin-associated nonmuscle myosin II (NM2) motor proteins play critical roles in a myriad of cellular functions, including endocytosis and organelle transport pathways. Cell type–specific expression unique subcellular localization the NM2 proteins, encoded by Myh9 Myh10 genes, mouse kidney tubules led us to hypothesize that these have specialized functional within renal epithelium. Inducible conditional knockout (cKO) adult mice resulted progressive disease. Prior overt tubular injury, we...
The diverse epithelial cell types of the kidneys are segregated into nephron segments and collecting ducts in order to endow each tubular segment with unique functions. rich diversity is highlighted by membrane channels receptors expressed within segment. Our previous work identified a critical role for Myh9 Myh10 mammalian endocytosis. Here, we examined expression patterns Nonmuscle myosin 2 (NM2) heavy chains encoded Myh9, Myh10, Myh14 mouse as these genes may confer segment-specific...
Abstract Type II myosin motors generate cytoskeletal forces that are central to cell division, embryogenesis, muscle contraction, and many other cellular functions. However, at present there is no method can directly measure the generated by myosins in living cells. Here we describe a Förster resonance energy transfer (FRET)-based tension sensor Nonmuscle Myosin IIB (NMIIB) cells with piconewton (pN) sensitivity. Fluorescence lifetime imaging microscopy (FLIM)-FRET measurements indicate...
SUMMARY Pancreatic islets are 3-dimensional cell aggregates consisting of unique cellular composition, cell-to-cell contacts, and interactions with blood vessels. Cell aggregation is essential for islet endocrine function; however, it remains unclear how developing establish aggregation. By combining genetic animal models, imaging tools, gene expression profiling, we demonstrate that regulated by extracellular matrix signaling cell-cell adhesion. Islet cell-specific inactivation receptor...
Introduction. This study describes the identification and partial characterization of persistence-inducing factors (PIFs) from staphylococci. Hypothesis/Gap Statement. Increases in persisters during mid-log phase growth indicate that quorum-sensing might be produced by Aim. To identify partially characterize PIFs Staphylococcus epidermidis RP62A aureus SH1000. Methodology. Others have demonstrated a significant increase persister numbers phase. Inducers this yet to identified Optical density...
Mutations that alter the trafficking and function of uromodulin, a membrane associated GPI anchored protein expressed in TAL segment, result autosomal dominant tubulointerstitial kidney disease (ADTKD)1. Proper localization GPI‐anchored proteins requires intact ER to Golgi apical transport. Recently Nonmuscle myosin II (NM2) have been linked vesicular transport2, we identified novel for NM2 isoforms Myh9 (NM2A) Myh10 (NM2B) regulating remodeling mammalian cells3. Due differential expression...
Nonmuscle Myosin 2 (NM2) is an actin associated motor protein that essential for cellular processes like adhesion, migration, polarity, and division. The NM2 family composed of three isoforms, NM2A, NM2B, NM2C encoded by genes Myh9 , Myh10 Myh14 respectively. Each these isoforms have both unique redundant functions due to differences in their affinities ATPase activities. Our previous work demonstrated a novel role Myh9&10 compensatory constitutive clathrin mediated endocytosis. Previous...
Abstract Background This study describes the identification and partial characterization of persistence inducing factors (PIF) from Staphylococcus aureus (S. aureus) epidermidis epidermidis). Persistence is an epigenetic process that results in tolerance bacterial cells to antibiotic treatment, which can result chronic human infections. Methods Others have demonstrated a significant increase persister numbers during mid-log phase. Inducers this yet be identified staphylococci. Optical...