Integral membrane proteins of Treponema pallidum subsp. (T. pallidum) were identified by phase partitioning with the nonionic detergent Triton X-114; antigens apparent molecular masses 47, 38, 36, 34, 32, 17, and 15 kilodaltons (kDa) in phase. Immunoblotting murine monoclonal antibodies directed against pathogen-specific 47- 34-kDa T. confirmed their presence Endoflagellar not detected immunoblots detergent-phase when monospecific antisera endoflagella nonpathogenic phagedenis biotype Reiter...

A number of the major pathogen-specific immunogens Treponema pallidum were characterized recently as amphiphilic, integral membrane proteins by phase partitioning with Triton X-114 (J. D. Radolf, N. R. Chamberlain, A. Clausell, and M. V. Norgard. Infect. Immun. 56:490-498, 1988). In present study, we demonstrated that same (designated detergent [DPPs]) become radiolabeled upon in vitro incubation T. various 3H-labeled fatty acids. Radioimmunoprecipitation a monoclonal antibody confirmed...

Heat-shock proteins are essential for stress tolerance and allowing organisms to survive conditions that cause protein unfolding. The role of the Staphylococcus aureus DnaK system in various stresses was studied by disruption dnaK partial deletion insertion a kanamycin gene cassette. Deletion S. strain COL resulted poor growth at temperatures 37 °C above, reduced carotenoid production. mutant also exhibited increased susceptibility oxidative cell-wall-active antibiotic conditions. In...

ABSTRACT Staphylococcus aureus is a major community and nosocomial pathogen. Its ability to withstand multiple stress conditions quickly develop resistance antibiotics complicates the control of staphylococcal infections. Adaptation lower temperatures key for survival bacterial species outside host. Branched-chain α-keto acid dehydrogenase (BKD) an enzyme complex that catalyzes early stages branched-chain fatty (BCFA) production. In this study, BKD was inactivated, resulting in reduced...

Staphylococcus aureus is susceptible to killing by host-derived fatty acids. Studies were performed test for a correlation between carotenoid production S. and protection against oleic acid. Oleic acid of cells grown in expression medium was determined as the dosage 2 M NaCl-2 mM EDTA that would kill 20% 60 min at 37 degrees C (i.e., lethal dose). Compared with wild-type strain (18Z), carotenoid-deficient mutant (18Z-76) 18Z suppressed both showed increased sensitivity Spontaneous revertants...

The pathogenesis of Staphylococcus epidermidis in foreign device-related infections is attributed primarily to its ability form biofilms on a polymer surface. One mechanism proposed for the survival organisms biofilm presence persister cells. Persister cells survive antibiotic treatment without acquiring heritable resistance. This study was conducted determine if S. RP62a growing planktonic cultures and could as following with levofloxacin vancomycin. produced small percentage persisters...

Summary: The growth of several adenine-independent strains yeasts in defined media containing suboptimal concentrations biotin and relatively high methionine is associated with the formation a pink pigment cells an arylamine culture fluid. Under similar conditions, are not produced by adenine-requiring strain Schizosaccharomyces octosporus. Culture filtrates promote Sch. octosporus adenine-free medium, but lactobacillus responds only after considerable lag. results interpreted to mean that...

Monoclonal antibodies directed against the 47-kilodalton (kDa) major outer membrane surface immunogen of virulent Treponema pallidum subsp. were used to select Escherichia coli recombinant clones expressing 47-kDa immunogen. The phenotype was dependent on presence plasmid in host cell. Southern hybridization revealed that cloned T. DNA sequence an accurate representation genomic arrangement. Purified immunoglobulin G from rabbits experimentally infected with and human secondary syphilitic...

Staphylococcus aureus is responsible for a wide variety of infections that include superficial skin and soft tissue infections, septicaemia, central nervous system endocarditis, osteomyelitis pneumonia. Others have demonstrated the importance toxin–antitoxin (TA) modules in formation persisters role Clp proteolytic regulation these TA modules. This study was conducted to determine effect clpP clpC deletion on S. persister cell numbers following antibiotic treatment. Deletion resulted...

A diazotizable amine and hypoxanthine accumulate in cultures of Yeast 47 grown a defined medium containing added methionine suboptimal biotin. Amine accumulation is depressed by the presence either adenine, or certain amino acids, which aspartate, norleucine, norvaline threonine are most effective. The differs from 4-amino-5-imidazolecarboxamide corresponding amidine. Spectrophotometric measurements suggest that lacks completed 6-membered ring structure purine skeleton, but it has biological...

The complete primary amino acid sequence for the 47-kilodalton (kDa) major integral membrane immunogen of Treponema pallidum subsp. was obtained by using a combined strategy DNA sequencing (of cloned gene in Escherichia coli) and N-terminal native (T. pallidum-derived) antigen. An open reading frame believed to encode 47-kDa antigen comprised 367 codons, which gave rise calculated molecular weight corresponding 40,701. Of acids, 113 (31%) were sequenced trypsin hydroxylamine cleavage...

The 47-kilodalton (kDa) major integral membrane immunogen of Treponema pallidum was recently found to be a proteolipid. Similar two-dimensional electrophoretic mobilities and common hydrophobic properties displayed by the native (T. pallidum) recombinant (Escherichia coli) 47-kDa antigens suggested that antigen also possesses covalently bound lipid. Both intact E. coli minicells acylated antigen; immunoprecipitation with monoclonal antibody specific for supported contention product...

The major outer membrane protein (P2) of Haemophilus influenzae type b (Hib) with an apparent molecular weight 37,000 to 40,000 has been previously shown function as a porin and also target for antibodies protective against experimental Hib disease. gene encoding the P2 was cloned by using shuttle vector capable replication in both Escherichia coli H. influenzae. amino acid sequence terminus determined used design oligonucleotide probe corresponding first 20 acids this protein. This identify...

The production of fatty-acid modifying enzyme (FAME) - first identified as a possible virulence factor in Staphylococcus aureus has also been S. epidermidis. This extracellular inactivates bactericidal fatty acids by esterifying them to cholesterol. FAME may provide protection for epidermidis inactivating these lipids present on the skin. Over 88% 51 randomly collected isolates produced FAME; 92.2% and 13.7% same strains lipase slime, respectively. There appeared be no correlation activity...

Previous work has established the importance of 47-kilodalton (kDa) surface immunogen Treponema pallidum subsp. (T. pallidum) in immunopathogenesis syphilis; 47-kDa gene was cloned and expressed Escherichia coli (M. V. Norgard, N. R. Chamberlain, M. A. Swancutt, S. Goldberg, Infect. Immun. 54:500-506, 1986). To facilitate additional structural-functional analysis this protein for studies, recombinant DNA-derived molecule examined with respect to its genetic expression physicochemical...

Pathogen-specific membrane immunogens of Treponema pallidum subsp. (T. pallidum) have been identified previously by phase partitioning with the nonionic detergent Triton X-114. One these antigens, a 15-kilodalton (kDa) polypeptide, is expressed in relatively small quantities T. but highly immunogenic both human and experimental syphilis. The native antigen was purified to homogeneity from mixture X-114 detergent-phase proteins chromatofocusing. Recombinant Escherichia coli clones were...

Background : Small-group case presentation exercises (CPs) were created to increase course relevance for medical students taking Medical Microbiology (MM) and Infectious Diseases (ID) Methods Each student received a unique paper had 10 minutes review patient history, physical exam data, laboratory data. Students then three orally present their defend why they ruled in or out each of the answer choices provided, followed by an additional questions. Results Exam scores differed significantly...

Fatty acid modifying enzyme (FAME) is an extracellular that inactivates staphylocidal lipids by catalysing the esterification of these to cholesterol. In-vitro expression FAME began at start stationary phase. This was very similar other staphylococcal proteins controlled global regulators Agr and Sar. A Staphylococcus aureus strain ISP546 (Agr−) produced c. 80% less than isogenic Agr+ ISP479C. Similar results were obtained with Agr+/Agr− pair RN6390 RN6911. S. R (Sar−) 86% Sar+ RN6390....

Abstract A number of Streptomyces strains producing streptothricin‐like antibiotics have been examined, and their products compared with known members this group. Circular paper chromatography has indicated that these are mixtures up to six components high antibacterial antifungal activity. Components separated by column tested against powdery mildews on plants showed eradicant protective action. Very antimildew activity was found in a major component D also F which corresponded...

Summary Three Staphylococcus aureus strains (303, 18Z and TG), exhibiting various patterns of survival within abscesses, were significantly more sensitive to the bactericidal activity oleic acid during log phase growth than at other stages cycle. Cells entering stationary showed diminished sensitivity fatty acid. These changes reflected by in LD50 also differences rate killing Additional noted: The declined over a 4-day period; progressively greater proportion staphylococcal population...