A5078135954- Sphingolipid Metabolism and Signaling
- Lipid Membrane Structure and Behavior
- Alzheimer's disease research and treatments
- Endoplasmic Reticulum Stress and Disease
- Neonatal Health and Biochemistry
- Amyotrophic Lateral Sclerosis Research
- Phagocytosis and Immune Regulation
- Liver Disease Diagnosis and Treatment
- Biochemical Acid Research Studies
- Cell death mechanisms and regulation
- Inflammasome and immune disorders
- Erythrocyte Function and Pathophysiology
- Neuroscience and Neuropharmacology Research
- Parkinson's Disease Mechanisms and Treatments
- Mitochondrial Function and Pathology
- Lysosomal Storage Disorders Research
- Lipid metabolism and disorders
- Cancer, Lipids, and Metabolism
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Protein Kinase Regulation and GTPase Signaling
- Metabolomics and Mass Spectrometry Studies
- Drug-Induced Hepatotoxicity and Protection
- Eicosanoids and Hypertension Pharmacology
- Heme Oxygenase-1 and Carbon Monoxide
- Biochemical effects in animals
Institute of Biochemical Physics NM Emanuel
2007-2024
Russian Academy of Sciences
1990-2020
Johns Hopkins University
1995
Duke University
1992
Duke University Hospital
1992
Duke Medical Center
1992
Burroughs Wellcome Fund
1992
Triangle
1992
Alzheimer's disease (AD) is characterized by progressive decline in cognition, memory and intellect. It has been hypothesized that amyloid-β peptide (A-β) may have a prominent role neurodegeneration. Oxidative mechanisms implicated this pathway. There substantial evidence inflammatory mechanisms, induced tumour necrosis factor α (TNF-α), are also involved AD. TNF-α activates receptors linked to multiple effector systems, including sphingomyelin pathway peroxide oxidation. We determined the...
Sphingosine displays multiple biochemical and biological effects, in particular inhibition activation of protein kinases.To determine the predominant interaction sphingosine with cellular kinases, effects on endogenous phosphorylation Jurkat T lymphoblastic cells were investigated vitro.Sphingosine was found to cause prominent a number cytosolic proteins ranging molecular mass from 18 165 kDa.Phosphorylation calcium-independent.Phosphorylation substrates increased response concentrations as...
Cycloheximide in sublethal doses caused apoptosis liver cells vivo, inducing c‐ myc , fos jun and p53 genes accumulation of sphingosine, a toxic product the sphingomyelin cycle. These data support hypothesis that continuous synthesis labile protective proteins is required to restrain liver; sphingosine might be important mediating cycloheximide‐induced as an endogenous modulator protein kinase C activity.
We used animal models to study connection between oxidating system and sphingomyelin signaling cascade, because this are more close related people disease. Activation of n‐sphingomyelinase (n‐SMase) in mice liver brain is coincided time with increased level peroxide products (conjugated dienes) after injection tumor necrosis factor α (TNF‐α). found that ceramide can induce oxidation lead accumulation TNF‐α organs. Nitric oxide (NO) donors ( S ‐nitrosoglutathione dinitrosyl iron complex)...
The review discusses the functional role of sphingolipids in pathogenesis Alzheimer's disease. Certain evidence exist that imbalance such as sphingomyelin, ceramide, sphingosine, sphingosine-1-phosphate and galactosylceramide brain animals humans, cerebrospinal fluid blood plasma patients with disease play a crucial neuronal function by regulating growth, differentiation cell death CNS. Activation sphingomyelinase, which leads to accumulation proapoptotic agent, can be considered new...
Glucan linked to proteins is a natural mega-glycoconjugate (mGC) playing the central role as structural component of yeast cell wall (CW). Regulation functioning non-covalently bound glucanosyltransglycosylases (ncGTGs) that have remodel mGC provide CW extension poorly understood. We demonstrate main ncGTGs Bgl2 and Scw4 phosphorylated glutathionylated residues are represented in different pools molecules having various firmness attachment. Identified contain with unmodified peptides, but...
Parkinson's disease (PD) is a neurodegenerative incurable due to late diagnosis and treatment. Therefore, one of the priorities neurology study mechanisms PD pathogenesis at preclinical early clinical stages. Given important role sphingolipids in diseases, we aimed analyze gene expression key sphingolipid metabolism enzymes (ASAH1, ASAH2, CERS1, CERS3, CERS5, GBA1, SMPD1, SMPD2, UGCG) content 32 (subspecies ceramides, sphingomyelins, monohexosylceramides sphinganine, sphingosine,...
Lipid metabolism disorders are the most significant risk factor of development cardiovascular diseases (CVD). In process diagnosing ischemic heart disease and other pathologies, levels total cholesterol, low- high- density lipoprotein triglycerides determined. However, in recent years, close attention has been paid to intersection metabolic pathways biosynthesis cholesterol sphingolipids. Sphingolipids - a group lipids, which include molecule aliphatic alcohol sphingosine. This includes...
Background: The aim of our study was to determine the role sphingolipids, which control proliferation and apoptosis, in placenta pregnant women with pregnancy-associated breast cancer (PABC) after chemotherapy compared healthy patients. Methods: We analyzed (by PCR method) gene expression key sphingolipid metabolism enzymes (sphingomyelinases (SMPD1 SMPD3), acid ceramidase (ASAH1), ceramide synthases (CERS 1–6), sphingosine kinase1 (SPHK1), sphingosine-1-phosphate lyase 1 (SGPL1), receptors...
Dimebon (Dimebolin) is an antihistamine drug which has been used in Russia since 1983. Recently Dimebolin attracted renewed interest after being shown to have positive effects on persons suffering from Alzheimer's disease. Animal studies that dimebon acts through multiple mechanisms, both blocking the action of neurotoxic beta-amyloid peptides and inhibiting L-type calcium channels, modulating AMPA NMDA glutamate receptors. Our experiments with cell culture L929 mice may exert its...