A5022461497- Immune Response and Inflammation
- Barrier Structure and Function Studies
- Helicobacter pylori-related gastroenterology studies
- Gut microbiota and health
- Cancer-related molecular mechanisms research
- Bladder and Urothelial Cancer Treatments
- Inflammatory mediators and NSAID effects
- MicroRNA in disease regulation
- Oral microbiology and periodontitis research
- Probiotics and Fermented Foods
- Cancer, Hypoxia, and Metabolism
- Urological Disorders and Treatments
- Urinary and Genital Oncology Studies
- Clinical Nutrition and Gastroenterology
- Renal and related cancers
- Nanoparticle-Based Drug Delivery
- Renal cell carcinoma treatment
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Nanoplatforms for cancer theranostics
- Plant Pathogenic Bacteria Studies
- Dental materials and restorations
- Immune cells in cancer
- Single-cell and spatial transcriptomics
- Ferroptosis and cancer prognosis
Kaohsiung Veterans General Hospital
2015-2025
National Taiwan University
2008-2025
National Taiwan University Hospital
2014-2025
National Yang Ming Chiao Tung University
2009-2024
National Defense Medical Center
2016-2024
Brigham and Women's Hospital
2017-2024
Harvard University
2017-2024
Taichung Armed Forces General Hospital
2024
Tri-Service General Hospital
2016-2024
Tatung University
2021
Cancer cells may survive under oxygen and nutrient deprivation by metabolic reprogramming for high levels of anaerobic glycolysis, which contributes to tumor growth drug resistance. Abnormally expressed glucose transporters (GLUTs) are colocalized with hypoxia (Hx) inducible factor (HIF)1α in peri-necrotic regions human colorectal carcinoma. However, the underlying mechanisms anti-necrotic resistance conferred metabolism hypoxic cancer remain poorly understood. Our aim was investigate...
The tight junction protein claudin-2 is upregulated in disease. Although many studies have linked intestinal barrier loss to local and systemic disease, these relied on macromolecular probes. In vitro analyses show, however, that probes cannot be accommodated by size- charge-selective channels. We sought define the impact of channels Transgenic overexpression or IL-13–induced upregulation increased small cation permeability vivo. IL-13 did not, affect claudin-2–knockout mice. Claudin-2...
Polarized epithelia assemble into sheets that compartmentalize organs and generate tissue barriers by integrating apical surfaces a single, unified structure. This organization is shared across organs, species, developmental stages. The processes regulate development maintenance of epithelial are, however, undefined. Here, using an intestinal epithelial-specific knockout (KO) mouse cultured cells, we show the tight junction scaffolding protein zonula occludens-1 (ZO-1) essential for in vivo...
Epithelial regeneration is essential for homeostasis and repair of the mucosal barrier. In context infectious immune-mediated intestinal disease, interleukin (IL) 22 thought to augment these processes. We sought define mechanisms by which IL22 promotes healing.Intestinal stem cell cultures mice were treated with recombinant IL22. Cell proliferation, death, differentiation assessed in vitro vivo morphometric analysis, quantitative reverse transcriptase polymerase chain reaction,...
Antibiotic usage promotes intestinal colonization of antibiotic-resistant bacteria. However, whether resistant bacteria gain dominance in enteric microflora or disseminate to extraintestinal viscera remains unclear. Our aim was investigate temporal diversity changes microbiota and transepithelial routes bacterial translocation after enterobacterial colonization. Mice drinking water with without antibiotics were intragastrically gavaged ampicillin-resistant (Amp-r) nonpathogenic Escherichia...
MicroRNAs (miRNAs) are small non-coding RNAs that have crucial regulatory functions in carcinogenesis. miR-324-5p and miR-324-3p generated from the same hairpin RNA structure, however, both diverse their direct target genes expression levels. We report of -3p was frequently observed to be either up-regulated or down-regulated, selection preference miR-324 for 5p 3p arms significantly varied various types human cancer. Overexpression suppressed growth invasion breast cancer cells. reduced...
Colorectal carcinogenesis is affected by overexpression of the lipopolysaccharide (LPS) receptors CD14 and TLR4, which antagonize each other affecting epithelial cell proliferation apoptosis. Eritoran an investigational drug for sepsis treatment that resembles lipid A moiety LPS therefore acts as a TLR4 inhibitor. In present study, we explored potential therapeutic uses mechanisms action eritoran in reducing colon cancer progression. administration via intracolonic, intragastric, or...
Objective Intestinal barrier loss is a Crohn’s disease (CD) risk factor. This may be related to increased expression and enzymatic activation of myosin light chain kinase 1 (MLCK1), which increases intestinal paracellular permeability correlates with CD severity. Moreover, preclinical studies have shown that MLCK1 recruitment cell junctions required for tumour necrosis factor (TNF)-induced as well experimental inflammatory bowel progression. We sought define mechanisms target this process...
Objectives: Titanium implants are regarded as a promising treatment modality for replacing missing teeth. Osteointegration and antibacterial properties both desirable characteristics titanium dental implants. The aim of this study was to create zinc (Zn)-, strontium (Sr)-, magnesium (Mg)-multidoped hydroxyapatite (HAp) porous coatings, including HAp, Zn-doped Zn-Sr-Mg-doped on discs using the vapor-induced pore-forming atmospheric plasma spraying (VIPF-APS) technique. Methods: mRNA protein...
Cyclic di-GMP (c-di-GMP) is a novel secondary-messenger molecule that involved in regulating plethora of important bacterial activities through binding to an unprecedented array effectors. Proteins with canonical PilZ domain bind c-di-GMP play crucial roles flagellum-based motility. In contrast, noncanonical type II domains do not effectively regulate twitching motility, which dependent on IV pili (T4P). Recent data indicate T4P biogenesis initiated via the interaction protein...
Abnormal bacterial adherence and internalization in enterocytes have been documented Crohn disease, celiac surgical stress, intestinal obstruction are associated with low-level interferon (IFN)-γ production. How commensals gain access to epithelial soma through densely packed microvilli rooted on the terminal web (TW) remains unclear. We investigated molecular ultrastructural mechanisms of endocytosis, focusing regulatory roles IFN-γ myosin light chain kinase (MLCK) TW phosphorylation brush...
Our study investigated how arecoline-induced extracellular vesicle (EV) secretion suppresses PAX1 protein production through DNA hypermethylation and examined whether downregulation enhances cancer stemness immunosuppression in the tumor microenvironment.
Abstract Sulfatases mediate the sulfation level of cell-surface heparan sulfates to regulate signal transduction, thereby promoting cancer progression. Sulfatase activation requires a sulfatase modifying factor (SUMF) for modification its catalytic domain. The role SUMF family in urothelial carcinoma (UC) has not been adequately evaluated. In this study, we used an online database and immunohistochemistry assess genetic changes mRNA protein expression SUMFs related candidate targets UC. We...
Cardiovascular diseases (CVDs) are some of the leading causes death and bypass surgery is one common treatment options for critical CVD patients. There no ideal material available arterial surgery. Herein, a fibroin keratin porous membrane was prepared by electro-spinning proposed tissue-engineered vascular grafts (TEVGs). The purified (F) (K) were mixed in different weight ratios 9 : 1 (FK91), 8 2 (FK82), 7 3 (FK73) to obtain membrane. SEM images revealed that electro-spinned membranes have...