A5101643010- Organ Transplantation Techniques and Outcomes
- Gut microbiota and health
- Clinical Nutrition and Gastroenterology
- Helicobacter pylori-related gastroenterology studies
- Immune Response and Inflammation
- Barrier Structure and Function Studies
- Cardiac Ischemia and Reperfusion
- Neurological Disease Mechanisms and Treatments
- Immune cells in cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Peptidase Inhibition and Analysis
- Laser Applications in Dentistry and Medicine
- Mitochondrial Function and Pathology
- Connexins and lens biology
- Liver Disease and Transplantation
- Anesthesia and Neurotoxicity Research
- Neurogenesis and neuroplasticity mechanisms
- Intestinal and Peritoneal Adhesions
- Signaling Pathways in Disease
- Pain Mechanisms and Treatments
- Pregnancy and preeclampsia studies
- Photoreceptor and optogenetics research
- Wound Healing and Treatments
- Tissue Engineering and Regenerative Medicine
- Retinal Development and Disorders
National Taiwan University
2007-2024
Australian Regenerative Medicine Institute
2020-2024
Monash University
2020-2024
Australian National University
2015-2018
National Chung Hsing University
2004-2006
Abstract The immune system has a critical role in orchestrating tissue healing. As result, regenerative strategies that control components have proved effective 1,2 . This is particularly relevant when dysregulation results from conditions such as diabetes or advanced age impairs healing following injury 2,3 Nociceptive sensory neurons crucial immunoregulators and exert both protective harmful effects depending on the context 4–12 However, how neuro–immune interactions affect repair...
Regulatory T cells (Tregs) are key immune regulators that have shown promise in enhancing cardiac repair post-MI, although the mechanisms remain elusive. Here, we show rapidly increasing Treg number circulation post-MI via systemic administration of exogenous Tregs improves function male mice, by limiting cardiomyocyte death and reducing fibrosis. Mechanistically, quickly home to infarcted heart adopt an injury-specific transcriptome mediates modulating monocytes/macrophages. Specially, lead...
Recent studies of Giardia lamblia outbreaks have indicated that 40-80% infected patients experience long-lasting functional gastrointestinal disorders after parasitic clearance. Our aim was to assess changes in the intestinal barrier and spatial distribution commensal bacteria post-clearance phase infection.Mice were orogastrically inoculated with G. trophozoites (strain GS/M) or pair-fed saline sacrificed on post-infective (PI) days 7 (colonization phase) 35 (post-clearance phase). Gut...
Abstract Chronic wounds are a major clinical problem where wound closure is prevented by pathologic factors, including immune dysregulation. To design efficient immunotherapies, an understanding of the key molecular pathways which immunity impairs healing needed. Interleukin-1 (IL-1) plays central role in regulating response to tissue injury through IL-1 receptor (IL-1R1). Generating knockout mouse model, we demonstrate that IL-1–IL-1R1 axis delays diabetic conditions. We used protein...
Scaffolds for bone defect treatment should ideally support vascularization and promote formation, to facilitate the translation into biomedical device applications. This study presents a novel approach utilizing 3D-printed water-dissolvable polyvinyl alcohol (PVA) sacrificial molds engineer polymerized High Internal Phase Emulsion (polyHIPE) scaffolds with microchannels distinct multiscale porosity. Two mold variants (250 µm 500 µm) were generated using fused deposition modeling, filled...
Objective: Gut barrier dysfunction and bacterial translocation occur in various disorders, including intestinal obstruction. Overexpression of inducible nitric oxide synthase is implicated the pathogenesis translocation, which molecular mechanism remains unclear. Epithelial permeability regulated by tight junction reorganization myosin light chain phosphorylation. Our aim was to investigate roles Rho-associated kinase protein C ζ epithelial synthase-mediated damage. Design: Animal study cell...
Among therapeutic proteins, cytokines and growth factors have great potential for regenerative medicine applications. However, these molecules encountered limited clinical success due to low effectiveness major safety concerns, highlighting the need develop better approaches that increase efficacy safety. Promising leverage how extracellular matrix (ECM) controls activity of during tissue healing. Using a protein motif screening strategy, we discovered amphiregulin possesses an exceptionally...
Aging-related changes of tubular cell apoptosis and its mechanisms in renal ischemia/reperfusion (I/R) injury are unclear. In the present study, aged (27-month-old) young (3-month-old) Wistar rats were used to investigate aging-related setting I/R injury. The model was induced by clamping bilateral arteries for 30 minutes followed reperfusion 18 hours. Cyclosporine A (CsA, 2 mg/kg) or mycophenolate mofetil (MMF, 20 mg/kg/d) before ischemia. Age-matched sham-operated served as controls. We...
A novel engineered form of interleukin-1 receptor antagonist augments the regenerative activity recombinant growth factors.
Bowel obstruction is a common cause of abdominal emergency, since the patients are at increased risk septicemia resulting in high mortality rate. While compartmentalized changes enteric microfloral population and augmentation bacterial translocation (BT) have already been reported using experimental models, alterations epithelial permeability obstructed guts has not studied detail. Myosin light chain kinase (MLCK) actively involved contraction perijunctional actinomyosin ring thereby...
To develop an in vivo intestinal permeability assay applying magnetic resonance imaging (MRI) to monitor real-time gut barrier defects animal models of acute mesenteric ischemia/reperfusion (I/R) insult.Twenty Wistar rats were divided 2 groups for I/R challenge or sham controls. received occlusion superior artery 20 minutes and reperfusion 1 hour. Sham-operation controls laparotomy without manipulation artery. assess permeability, a 10-cm jejunal sac was created distal the ligament Treitz...
Balloon-assisted enteroscopy (BAE) is an emerging standard procedure by utilizing distensible balloons to facilitate deep endoscopy in the small and large intestine. Sporadic cases of bacteraemia were found after BAE. Balloon distension BAE caused gut tissue hypoxia. The impact balloon distension-induced hypoxia on intestinal barriers remains unclear. Murine models colonic showed that short- long-term evoked opposite effects epithelial tight junctions (TJs). Short-term fortified TJ...
Purpose: Light is a requirement for the function of photoreceptors in visual processing. However, prolonged light exposure can be toxic to photoreceptors, leading increased reactive oxygen species (ROS), lipid peroxidation, and photoreceptor cell death. We used 661W mouse cone photoreceptor-like line study effects pyruvate protecting these cells from light-induced toxicity.Methods: were exposed 15,000 lux continuous bright 5 hours incubated Dulbecco's modified eagle medium (DMEM) with...