A5080144361- Neonatal Respiratory Health Research
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Congenital Diaphragmatic Hernia Studies
- Preterm Birth and Chorioamnionitis
- Congenital Anomalies and Fetal Surgery
- Extracellular vesicles in disease
- Muscle Physiology and Disorders
- T-cell and B-cell Immunology
- Thermal Regulation in Medicine
- Immune Response and Inflammation
- Non-Invasive Vital Sign Monitoring
- CAR-T cell therapy research
- Cardiac Fibrosis and Remodeling
- Hearing, Cochlea, Tinnitus, Genetics
- Energy Harvesting in Wireless Networks
- Autophagy in Disease and Therapy
- Tendon Structure and Treatment
- COVID-19 Impact on Reproduction
- Neuroinflammation and Neurodegeneration Mechanisms
- Fibroblast Growth Factor Research
- Fatty Acid Research and Health
- Reproductive System and Pregnancy
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Corneal Surgery and Treatments
Australian Regenerative Medicine Institute
2018-2024
Monash University
2013-2024
Hudson Institute of Medical Research
2016-2023
EMBL Australia
2021
Monash Medical Centre
2014-2016
Monash Institute of Medical Research
2012-2015
Hudson Institute
2015
Seattle Children's Hospital
2010
Idiopathic pulmonary fibrosis (IPF) is characterized by chronic inflammation, severe scarring, and stem cell senescence. Stem cell-based therapies modulate inflammatory fibrogenic pathways release of soluble factors. cell-derived extracellular vesicles should be explored as a potential therapy for IPF. Human amnion epithelial exosomes (hAEC Exo) were isolated compared against human lung fibroblasts exosomes. hAEC Exo assessed fibrosis. Exosomes evaluated their protein miRNA cargo. Direct...
Abstract Bronchopulmonary dysplasia (BPD) is a chronic lung disease that mainly affects premature babies who require ventilator support. The pathogenesis of BPD complex but includes vascular maldevelopment, alveolarization arrest, and inflammation. There no cure for BPD. Clinical care limited to supportive respiratory measures. A population stem-like cells derived from placental membranes, human amnion epithelial (hAECs), has shown therapeutic promise in preclinical models With view future...
Human amnion epithelial cells (hAECs) have been shown to modulate inflammation and restore normal lung structure respiratory function following bleomycin challenge in immune-competent mice. These effects are exerted despite a lack of significant engraftment hAECs, suggesting that immunomodulatory effect mechanisms at play. In this study, using the model injury, we explored interactions between hAECs macrophages. We administered 4 million intraperitoneally C57Bl6 mice 24 h challenge. Using...
Regulatory T cells (Tregs) are key immune regulators that have shown promise in enhancing cardiac repair post-MI, although the mechanisms remain elusive. Here, we show rapidly increasing Treg number circulation post-MI via systemic administration of exogenous Tregs improves function male mice, by limiting cardiomyocyte death and reducing fibrosis. Mechanistically, quickly home to infarcted heart adopt an injury-specific transcriptome mediates modulating monocytes/macrophages. Specially, lead...
The immunomodulatory properties of human amnion epithelial cells (hAECs) have been previously described in several disease models. We reported on the ability hAECs to influence macrophage phenotype and chemotaxis. In this study, we aim elucidate contribution regulatory T (Tregs) polarisation downstream effects inflammation fibrosis a bleomycin model lung injury.Either CD45(+)/FoxP3(+) Tregs or CD45(+)/FoxP3 (-) non-Tregs were adoptively transferred into Rag1 (-/-) mice immediately prior...
Abstract Chronic wounds are a major clinical problem where wound closure is prevented by pathologic factors, including immune dysregulation. To design efficient immunotherapies, an understanding of the key molecular pathways which immunity impairs healing needed. Interleukin-1 (IL-1) plays central role in regulating response to tissue injury through IL-1 receptor (IL-1R1). Generating knockout mouse model, we demonstrate that IL-1–IL-1R1 axis delays diabetic conditions. We used protein...
Mesenchymal stromal cells (MSCs) have significant therapeutic potential due to their ability differentiate into musculoskeletal lineages suitable for tissue-engineering, as well the immunomodulatory and pro-regenerative effects of paracrine factors that these secrete. Cues from extracellular environment, including physical stimuli such substrate stiffness, are strong drivers MSC differentiation, but upon activity not understood. This study, therefore sought determine impact stiffness on...
Since current treatments for both acute and chronic lung diseases are less than ideal, there has been recent interest in the use of cell-based therapies inflammatory disease. Specifically, human amnion epithelial cells (hAECs) have shown to reduce bleomycin-induced injury prevent subsequent loss respiratory function, primarily through modulation host immune response. The precise mechanisms this effect remain unclear. We aimed investigate potential hAECs mitigate surfactant protein C...
Respiratory dysfunction is one of the leading causes morbidity and mortality in world rates continue to rise. Quantitative assessment lung function rodent models an important tool development future therapies. Commonly used techniques for assessing respiratory including invasive plethysmography forced oscillation. While these provide valuable information, data collection can be fraught with artefacts experimental variability due need anesthesia and/or instrumentation animal. In contrast,...
Despite advances in neonatal care, bronchopulmonary dysplasia (BPD) remains a significant contributor to infant mortality and morbidity. While human amnion epithelial cells (hAECs) have shown promise small large animal models of BPD, there is scarce information on long-term benefit clinically relevant questions surrounding administration strategy remain unanswered. In assessing the therapeutic potential hAECs, we investigated impact cell dosage, routes timing treatment pre-clinical model...
Human amnion epithelial cells (hAECs) are clonogenic and have been proposed to reduce inflammatory-induced tissue injury. Perturbation of the immune response is implicated in pathogenesis perinatal brain injury; modulating this could thus be a novel therapy for treating or preventing such The immunomodulatory properties hAECs shown other animal models, but detailed investigation effects on following injury has not undertaken. Here, we investigate microglia, first responders within brain. We...
Respiratory dysfunction is one of the leading causes morbidity and mortality in world rates continue to rise. Quantitative assessment lung function rodent models an important tool development future therapies. Commonly used techniques for assessing respiratory including invasive plethysmography forced oscillation. While these provide valuable information, data collection can be fraught with artefacts experimental variability due need anesthesia and/or instrumentation animal. In contrast,...
Abstract Human amnion epithelial cells (hAECs) have been shown to possess potent immunomodulatory properties across a number of disease models. Recently, we reported that hAECs influence macrophage polarization and activity, this step was dependent on regulatory T cells. In study, aimed assess the effects hAEC-derived proresolution lipoxin-A4 (LXA4) T-cell, macrophage, neutrophil phenotype function during acute phase bleomycin-induced lung injury. Using C57Bl6 mice, administered 4 million...
Perivascular mesenchymal stem/stromal cells can be isolated from the human endometrium using surface marker SUSD2 and are being investigated for use in tissue repair. Mesenchymal other tissues modulate T cell responses via mechanisms including interleukin-10, prostaglandin E2, TGF-β1 regulatory cells. Animal studies demonstrate that endometrial also modify immune to implanted mesh, but mechanism/s they employ have not been explored. We examined immunomodulatory properties of on lymphocyte...
Abstract There is a growing appreciation of the role lung stem/progenitor cells in development and perpetuation chronic disease including idiopathic pulmonary fibrosis. Human amniotic epithelial (hAECs) were previously shown to improve architecture bleomycin-induced injury, with further suggestion that hAECs obtained from term pregnancies possessed superior anti-fibrotic properties compared their preterm counterparts. In present study, we aimed elucidate differential effects on involved...
<h3>Introduction</h3> Ex-vivo lung perfusion (EVLP) is used to assess and potentially recondition donor lungs that are not initially suitable for transplantation. In a recent UK study, EVLP was associated with higher primary graft dysfunction (PGD) rates compared standard evaluation procedure. PGD process characterised by leucocyte-endothelial adhesion acute injury. this leucocyte content endothelial activation before after assessed then the potential of human amnion epithelial cell (hAEC)...