A5044772658- Muscle Physiology and Disorders
- Cell Adhesion Molecules Research
- Signaling Pathways in Disease
- Zebrafish Biomedical Research Applications
- Congenital heart defects research
- Developmental Biology and Gene Regulation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cellular Mechanics and Interactions
- Adipose Tissue and Metabolism
- COVID-19 and healthcare impacts
- Bone and Dental Protein Studies
- Autophagy in Disease and Therapy
- Retinoids in leukemia and cellular processes
- Regulation of Appetite and Obesity
- SARS-CoV-2 detection and testing
- Calpain Protease Function and Regulation
- Nuclear Receptors and Signaling
- Tissue Engineering and Regenerative Medicine
- Barrier Structure and Function Studies
- Physiological and biochemical adaptations
- Health disparities and outcomes
- Respiratory Support and Mechanisms
- Congenital Diaphragmatic Hernia Studies
- Prosthetics and Rehabilitation Robotics
- RNA Research and Splicing
Australian Regenerative Medicine Institute
2014-2022
Monash University
2016-2022
EMBL Australia
2020-2021
Imperial College Healthcare NHS Trust
2016
Homerton University Hospital NHS Foundation Trust
2013
Newcastle University
2010-2012
Centre for Life
2011
Medical Research Council
1996
University of British Columbia
1956-1961
Abstract The muscular dystrophies encompass a broad range of pathologies with varied clinical outcomes. In the case patients carrying defects in fukutin-related protein (FKRP), these diverse arise from mutations within same gene. This is surprising as FKRP glycosyltransferase, whose only identified function to transfer ribitol-5-phosphate α-dystroglycan (α-DG). Although this modification critical for extracellular matrix attachment, α-DG’s glycosylation status relates poorly disease...
Fukutin and fukutin-related protein (FKRP) are involved in the glycosylation of α-dystroglycan, a key receptor for basement membrane proteins. Aberrant α-dystroglycan leads to broad spectrum disorders, ranging from limb girdle muscular dystrophy Walker-Warburg syndrome. This is first study investigating role fukutin FKRP-mediated angiogenesis. Transgenic zebrafish expressing enhanced green fluorescent blood vessels were treated with morpholino antisense oligonucleotides that blocked...
Laminins comprise structural components of basement membranes, critical in the regulation differentiation, survival and migration a diverse range cell types, including skeletal muscle. Mutations one muscle enriched Laminin isoform, alpha2 (Lama2), results most common form congenital muscular dystrophy, dystrophy type 1A (MDC1A). However, exact cellular mechanism by which loss pathological spectrum associated with MDC1A remains elusive. Here we show, via live tracking individual fibres, that...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) biosensors have captured more attention than the conventional methodologies for SARS‐CoV‐2 detection due to having cost‐effective platforms and fast detection. However, these reported suffer from drawbacks including issues in sensitivity, degradation of biomaterials on sensor's surface, incapability reuse biosensors. To overcome shortcomings, molecularly imprinted polymer nanoparticles (nanoMIPs) incorporated...
Deficiency of muscle basement membrane (MBM) component laminin-α2 leads to muscular dystrophy congenital type 1A (MDC1A), a currently untreatable myopathy. Laminin--α2 has two main binding partners within the MBM, dystroglycan and integrin. Integrins coordinate both cell adhesion signalling; however, there is little mechanistic insight into integrin's function at MBM. In order study role in development how this relates MBM's capacity handle force, an itgβ1.b−/− zebrafish line was created....
Abstract Skeletal muscle is paradigmatic of a regenerative tissue that repairs itself via the activation resident stem cell 1 . Termed satellite cell, these normally quiescent cells are induced to proliferate by ill-defined wound-derived signals 2 Identifying source and nature pro-regenerative cues has been hampered an inability visualise complex cellular interactions occur within wound environment. We therefore developed zebrafish injury model systematically capture site, in real time,...
Abstract The Nuclear receptor subfamily 4 group A member 2 (Nr4a2) is crucial for the formation or maintenance of dopaminergic neurons in central nervous system including retina, where amacrine cells contribute to visual function. Little known about which express Nr4a2 at developmental stage. Furthermore, whether functions combination with other genes poorly understood. Thus, we generated a novel transgenic visualize expression vivo during zebrafish retinogenesis. 4.1 kb fragment nr4a2a...
THE present study analysed the susceptibility of neuronal cells to human complement. A precursor cell line (NTera2) which can be induced differentiate into post-mitotic was employed. Using immunocytochemistry, NTera2 neurones, but not their precursors (stem cells), were shown activate complement in absence antibody and lack CD59, an inhibitor terminal stages cascade. Following exposure serum, neurones stem lysed by complement, as demonstrated a viability assay consistent rise intracellular...
It has been demonstrated (Barber et al., 1951, Becker Bennison Braude Leuecke Barber 1952) that antibiotic supplementation of apparently adequate diets may result in an accelerated growth rate and improved feed efficiency animals consuming such diets. The practical application these results is already finding extensive use despite the fact common agreement as to fundamental mode action antibiotics yet be secured, under certain circumstances, improvement not obtained on supplemented
Muscle fiber detachment from myoseptal boundaries is a common finding in zebrafish models of muscular dystrophies. In some instances, there weakening the interaction between muscle and myosepta, which yet to manifest as phenotype. Therefore, push muscle, mutant fish but not their wild-type siblings, beyond binding threshold, series small electrical pulses can be applied larvae create maximal force contraction ultimately detachment. To do this, we built digital pulse generator delivers four 8...
The design of an interactive computer program in microbial genetics is described. divided into three stages: background information, questions, and the simulation. results simulated conjugation experiments with Escherichia coli allow eight chromosomal genes to be sequenced. These can mapped relative different sites F plasmid insertion transfer orientation. simulation requires student choose appropriate donor recipient strains bacterium, specify constituents selective media on which progeny...