A5066177252- Pancreatic function and diabetes
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Extracellular vesicles in disease
- Cell Adhesion Molecules Research
- Immune cells in cancer
- Platelet Disorders and Treatments
- Bacterial Infections and Vaccines
- Muscle Physiology and Disorders
- Ion-surface interactions and analysis
- Advanced Electron Microscopy Techniques and Applications
- Cardiomyopathy and Myosin Studies
- Lipid metabolism and biosynthesis
- Autophagy in Disease and Therapy
- Microscopic Colitis
- Cholesterol and Lipid Metabolism
- Calcium signaling and nucleotide metabolism
- Amoebic Infections and Treatments
- Pneumonia and Respiratory Infections
- Cellular Mechanics and Interactions
- Gastrointestinal disorders and treatments
- Nanopore and Nanochannel Transport Studies
- Growth Hormone and Insulin-like Growth Factors
- Immune Cell Function and Interaction
- Trypanosoma species research and implications
- Mass Spectrometry Techniques and Applications
Monash University
2014-2023
University of Oxford
2020-2022
The University of Sydney
2021
Australian Research Council
2020
The University of Queensland
2005-2011
Abstract Bacterial outer membrane vesicles (OMVs) are extracellular sacs containing biologically active products, such as proteins, cell wall components and toxins. OMVs reported to contain DNA, however, little is known about the nature of this nor whether it can be transported into host cells. Our work demonstrates that chromosomal DNA packaged shed by bacteria during exponential phase. Most was present on external surfaces OMVs, with smaller amounts located internally. The within internal...
Endocrine cells are continually regulating the balance between hormone biosynthesis, secretion, and intracellular degradation to ensure that cellular stores maintained at optimal levels. In pancreatic β-cells, insulin in β-granules mostly upheld by efficiently up-regulating proinsulin biosynthesis translational level rapidly replenish lost via exocytosis. Under normal circumstances, of plays a relatively minor janitorial role retiring aged β-granules, apparently crinophagy. However, this...
Neisseria gonorrhoeae causes the sexually transmitted disease gonorrhoea by evading innate immunity. Colonizing mucosa of reproductive tract depends on bacterial outer membrane porin, PorB, which is essential for ion and nutrient uptake. PorB also targeted to host mitochondria regulates apoptosis pathways promote infections. How traffics from N. whether it modulates immune cells, such as macrophages, remains unclear. Here, we show that secretes via vesicles (OMVs). Purified OMVs contained...
Abstract Aims Acute myocardial infarction rapidly increases blood neutrophils (<2 h). Release from bone marrow, in response to chemokine elevation, has been considered their source, but levels peak up 24 h after injury, and neutrophil elevation. This suggests that additional non-chemokine-dependent processes may be involved. Endothelial cell (EC) activation promotes the rapid (<30 min) release of extracellular vesicles (EVs), which have emerged as an important means cell–cell...
Abstract Despite tremendous advances in sample preparation and classification algorithms for electron cryomicroscopy (cryo-EM) single-particle analysis (SPA), heterogeneity remains a major challenge can prevent access to high-resolution structures. In addition, optimization of conditions given be time-consuming. the current work, it is demonstrated that native electrospray ion-beam deposition (native ES-IBD) an alternative, reliable approach extremely high-purity samples, based on mass...
OBJECTIVE The ATP-binding cassette transporter A1 (ABCA1) is essential for normal insulin secretion from β-cells. aim of this study was to elucidate the mechanisms underlying impaired in islets lacking β-cell ABCA1. RESEARCH DESIGN AND METHODS Calcium imaging, patch clamp, and membrane capacitance were used assess effect ABCA1 deficiency on calcium flux, ion channel function, exocytosis islet cells. Electron microscopy analyze ultrastructure. quantity distribution proteins involved...
Nemaline myopathy is characterized by muscle weakness and the presence of rod-like (nemaline) bodies. The genetic etiology nemaline becoming increasingly understood with mutations in ten genes now known to cause disease. Despite this, mechanism which skeletal occurs remains elusive, previous studies showing no correlation between frequency bodies disease severity. To investigate formation their role pathogenesis, we generated overexpression loss-of-function zebrafish models for α-actin...
Summary In G ram‐negative bacteria, β‐barrel proteins are integrated into the outer membrane by assembly machinery, with key components of machinery being O mp85 family members BamA and TamA . Recent crystal structures cryo‐electron microscopy show a diverse set secretion pores in α‐helix ( W za GspD ) or β‐strand CsgG transmembrane segments membrane. We developed assays to measure three distinct that mediate protein ), curli fibre capsular polysaccharide za) bacteria depletion does not...
Cell membrane thyroid hormone (TH) transport can be facilitated by the monocarboxylate transporter 8 (MCT8), encoded solute carrier family 16 member 2 (SLC16A2) gene. Human mutations of gene, SLC16A2, result in X-linked-inherited psychomotor retardation and hypomyelination disorder, Allan-Herndon-Dudley syndrome (AHDS). We posited that abrogating MCT8-dependent TH limits oligodendrogenesis myelination. show human oligodendrocytes (OL), derived from NKX2.1-GFP embryonic stem cell (hESC)...
Abstract Deciphering the molecular basis of leukocyte recruitment is critical to understanding inflammation. In this study, we investigated contribution tetraspanin CD37 key process. CD37-deficient mice showed impaired neutrophil in a peritonitis model. Intravital microscopic analysis indicated that absence capacity leukocytes follow CXCL1 chemotactic gradient accurately interstitium. Moreover, CXCL1-induced leukocyte-endothelial cell interactions postcapillary venules revealed adhesion and...
Deficiency of muscle basement membrane (MBM) component laminin-α2 leads to muscular dystrophy congenital type 1A (MDC1A), a currently untreatable myopathy. Laminin--α2 has two main binding partners within the MBM, dystroglycan and integrin. Integrins coordinate both cell adhesion signalling; however, there is little mechanistic insight into integrin's function at MBM. In order study role in development how this relates MBM's capacity handle force, an itgβ1.b−/− zebrafish line was created....
Abstract Electron cryomicroscopy (cryo-EM) and single-particle analysis (SPA) have revolutionized structure determination of homogeneous proteins. However, obtaining high-resolution structures from heterogeneous samples remains a major challenge, as the various protein states embedded in thin films vitreous ice may be classified incorrectly, resulting detrimental averaging features. Here we present native electrospray ion-beam deposition (native ES-IBD) for preparation extremely high-purity...
Abstract Objective The endothelial surface layer (ESL), a of macromolecules on the cells, can both impede and facilitate leukocyte recruitment. However, its role in monocyte neutrophil recruitment glomerular capillaries is unknown. Methods We used multiphoton intravital microscopy to examine behavior glomerulus following ESL disruption with hyaluronidase. Results Constitutive retention migration monocytes neutrophils within microvasculature was unaltered by Consistent this, inhibition...
Acute myocardial infarction rapidly increases blood neutrophils (<2 hours). Release of from bone marrow, in response to chemokine elevation, has been considered their source, but levels peak up 24 hours after injury, and neutrophil elevation. This suggests that additional non chemokine-dependent processes may be involved. Endothelial cell (EC) activation promotes the rapid (<30 minutes) release extracellular vesicles (EVs), which are enriched vascular adhesion molecule-1 (VCAM-1)...
Extract HTML view is not available for this content. However, as you have access to content, a full PDF via the ‘Save PDF’ action button. Extended abstract of paper presented at Microscopy and Microanalysis 2005 in Honolulu, Hawaii, USA, July 31--August 4,
Abstract Virtual memory T (TVM) cells are antigen-naïve CD8 cell that exist in a semi-differentiated state. They exhibit some characteristics classically associated with conventional (TMEM), including elevated expression of CD44, accelerated TCR-driven proliferation, and enhanced cytokine responsiveness. Despite rapid robust proliferation young TVM after TCR-mediated stimulation, aged marked proliferative dysfunction. Recently, high spare respiratory capacity (SRC) was proposed as defining...