MicroRNAs (miRNAs) are small noncoding RNAs that play fundamental roles in diverse biological and pathological processes by targeting the expression of specific genes. Here, we identified 38 methylation-associated miRNAs, which could be epigenetically restored cotreatment with 5-aza-2'-deoxycytidine trichostatin A. Among these further analyzed miR-34b, miR-127-3p, miR-129-3p miR-409 because CpG islands predicted adjacent to them. The methylation-silenced miRNAs reactivated gastric cancer...

Abstract Background MicroRNAs (miRNAs) are small non-protein-coding RNAs. miRNA genes need several biogenesis steps to form function miRNAs. However, the precise mechanism and biology involved in mature molecules not clearly investigated. In this study, we conducted in-depth analyses examine arm selection isomiRs using NGS platform. Methods We sequenced RNAs from one pair of normal gastric tumor tissues with Solexa By analyzing data, quantified expression profiles miRNAs tissues. Then,...

DNA and RNA samples from blood are the common examination target for non-invasive physical tests and/or biomedical studies. Since high-quality guarantee correctness of these studies, we investigated effects storage temperature duration whole on qualities. Subjects were enrolled to donate which stored different durations at temperatures, followed by examinations quality, qPCR, quality methylation. For RNA, observed obvious decline with longer than 24 hours. Storage low does not keep...

MicroRNAs (miRNAs) play crucial roles in various physiological processes through post-transcriptional regulation of gene expressions and are involved development, metabolism, many other important molecular mechanisms cellular processes. The Bombyx mori genome sequence provides opportunities for a thorough survey miRNAs as well comparative analyses with sequenced insect species.We identified 114 non-redundant conserved 148 novel putative from the B. an elaborate computational protocol. We...

Identification of microRNAs (miRNAs) is essential to studying their physiological functions. Due the difficulties in discovering truly expressed miRNAs from genomic random hairpin secondary structure sequences, it beneficial predict them sequences—expressed sequence tags (ESTs) and intronic sequences. We used a modified scanning pipeline using criteria based on features known pre-miRNAs phylogenetic conservation for predicting miRNAs. Upon examination, we found that 25% human belong regions...

Carcinogenesis of the stomach involves multiple steps including genetic mutation or epigenetic alteration tumor suppressor genes oncogenes. Recently, suppressive miRNAs have been shown to be deregulated by aberrant hypermethylation during gastric cancer progression. In this study, we demonstrate that three independent loci encoding for miR-9 (miR-9-1, miR-9-2 and miR-9-3) are simultaneously modified DNA methylation in cells. Methylation-mediated silencing these can reactivated cells through...

Abstract MicroRNAs (miRNAs) are short noncoding RNAs that play important roles in cellular processes and disease pathogenesis via the control of specific targeted gene expression. The miR‐196s miRNA is encoded at three paralogous loci HOX clusters acts as an oncogenic cancer progression. Recent studies have demonstrated expression miR‐196b increases cell proliferation survival leukemic cells. Here, we used a sequential methylation analysis to reveal status correlated well with different...

Abstract MicroRNAs (miRNAs) are short noncoding RNAs (˜22 nt) that play important roles in the pathogenesis of human diseases by negatively regulating gene expression. Here, we examined relationship between miR‐196a and gastric cancer. By analysis 72 cancer samples, found expression level microRNA significantly increased primary tissues versus adjacent normal tissues. In addition, extracellular detected conditioned medium was strongly correlated with its cellular status circulating patient...

MicroRNAs (miRNAs) are short noncoding RNAs derived from the 3' and 5' ends of same precursor. However, biological function mechanism miRNA arm expression preference remain unclear in breast cancer. We found significant decreases levels miR-193a-5p but no differences those miR-193a-3p MiR-193a-3p suppressed cancer cell growth migration invasion abilities, whereas did not influence motility. Furthermore, NLN CCND1, PLAU, SEPN1 were directly targeted by miR-193a-3p, respectively, cells. The...

Long noncoding RNAs (lncRNAs) are more than 200 nucleotides in length and lack transcriptional ability. The biological function of lncRNAs oral squamous cell carcinoma (OSCC) remains unclear. aim this study was to identify the dysfunction lncRNA OSCC. We analyzed transcriptome profiles human OSCC tissues paired adjacent normal from two patients through a next-generation sequencing approach. A total 14 were upregulated (fold change ≥3) 13 downregulated ≤−3) compared with tissues. SOX21-AS1...

MicroRNAs (miRNAs) are small non-coding RNAs that have crucial regulatory functions in carcinogenesis. miR-324-5p and miR-324-3p generated from the same hairpin RNA structure, however, both diverse their direct target genes expression levels. We report of -3p was frequently observed to be either up-regulated or down-regulated, selection preference miR-324 for 5p 3p arms significantly varied various types human cancer. Overexpression suppressed growth invasion breast cancer cells. reduced...

Melanoma is a highly aggressive cancer with high mortality in advanced stages.Metformin an oral biguanide drug used for diabetes and has demonstrated positive effects oncancer prevention treatment. Herein, we found that metformin significantly suppressedmelanoma cell motility growth through inducing cycle arrest at the G2/M phase andpromoting apoptosis. Using next-generation sequencing approach, identified threeupregulated microRNAs (miRNA; miR-192-5p, miR-584-3p, miR-1246) melanoma...

Abstract Attention-deficit/hyperactivity disorder (ADHD) is a common childhood mental with undetermined pathophysiological mechanisms. The gut microbiota and immunological dysfunction may influence brain functions social behaviours. In the current study, we aimed to explore correlation of microbiome imbalance inflammation in pathophysiology ADHD. Forty-one children ADHD thirty-nine healthy-control (HC) individuals were recruited. Faecal samples from all participants collected submitted for...

Background Dysbiosis in the gut microbial community might be involved pathophysiology of attention‐deficit/hyperactivity disorder (ADHD). The fungal component microbiome, namely mycobiota, is a hyperdiverse group multicellular eukaryotes that can influence host intestinal permeability. This study therefore aimed to investigate impact mycobiome dysbiosis and permeability on ADHD. Methods Faecal samples were collected from 35 children with ADHD healthy controls. Total DNA was extracted faecal...

Abstract Background MicroRNAs (miRNAs) are endogenous non-protein-coding RNA genes which exist in a wide variety of organisms, including animals, plants, virus and even unicellular organisms. Medaka ( Oryzias latipes ) is useful model organism among vertebrate animals. However, no medaka miRNAs have been investigated systematically. It beneficial to conduct genome-wide miRNA discovery study using the next generation sequencing (NGS) technology, has emerged as powerful tool for...

Gastric carcinogenesis is a complex multistep process involving genetic dysregulation of proto-oncogenes and tumorsuppressor genes, has recently entered the era microRNAs (miRNAs), class small non-coding RNAs that posttranscriptionally regulate gene expression control various cellular functions. MicroRNAs are (approximately 22 nucleotides) play fundamental roles in diverse biological pathological processes, including cell proliferation, differentiation, apoptosis, carcinogenesis. have been...

E26 transformation-specific sequence (ETS)-2 is a transcriptional modulator located on chromosome 21, alterations in its expression have been implicated with reduced incidence of solid tumors Down syndrome patients. MicroRNAs (miRNAs) are thought to participate diverse biological functions; however, the regulation miRNAs not well characterized. Recently, we reported that miR-196b highly expressed gastric cancers. Herein, demonstrate was significantly repressed by ETS2 during cancer...

Kawasaki disease (KD) or syndrome is known as a vasculitis of small to medium-sized vessels, and coronary arteries are predominantly involved in childhood. Generally, 20–25% untreated with IVIG 3–5% treated KD patients have been developed artery lesions (CALs), such dilatation aneurysm. Understanding how aneurysms (CAAs) established maintained therefore great importance. Upon our previous genotyping data 157 valid subjects, genome-wide association study (GWAS) has conducted among 11 (7%)...

MicroRNA-29 (miR-29) is found to modulate hepatic stellate cells’ (HSCs) activation and, thereby, reduces liver fibrosis pathogenesis. Histone methyltransferase regulation of epigenetic reactions reportedly participates in fibrosis. This study undertaken investigate the miR-29a signaling and program progression. transgenic mice (miR-29aTg mice) wild-type littermates were subjected bile duct-ligation (BDL) develop cholestatic Primary HSCs transfected with a mimic antisense inhibitor....

Abstract Background MicroRNAs (miRNAs) are short noncoding RNAs (approximately 22 nucleotides in length) that play important roles breast cancer progression by downregulating gene expression. The detailed mechanisms and biological functions of miRNA molecules carcinogenesis have yet to be fully elucidated. This study used bioinformatics experimental approaches conduct analysis the dysregulated miRNAs, arm selection preferences, 3' end modifications, position shifts isoforms miRNAs (isomiRs)...

The expression of microRNA (miRNA) genes undergoes several maturation steps. Recent studies brought new insights into the process, but also raised debates on mechanism. To understand mechanism better, we downloaded small RNA sequence reads from NCBI SRA and quantified profiles miRNAs in normal tumor liver tissues. From these miRNA profiles, studied issues related to biogenesis. First all, 3' ends mature usually carried modified nucleotides, generated nucleotide addition or editing. We found...