Hericium erinaceus, an edible mushroom, has been demonstrated to potentiate the effects of numerous biological activities. The aim this study was investigate whether H. erinaceus mycelium could act as anti-inflammatory agent bring about neuroprotection using a model global ischemic stroke and mechanisms involved. Rats were treated with its isolated diterpenoid derivative, erinacine A, after ischemia reperfusion brain injuries caused by occlusion two common carotid arteries. production...

Stromal cell-derived factor-1 (SDF-1) (CXC chemokine ligand-12)/CXC receptor 4 (CXCR4) is involved in the carcinogenesis of human gastric cancer, where it stimulates angiogenesis and favors metastasis tumor cells to distant organs. In addition, resistin suggested be an important link between obesity development cancer. Resistin has identified as player inflammatory responses, emerged a mediator inflammation-associated A limited number studies have investigated association SDF-1 with Herein,...

Toll-like receptors (TLRs) not only form an important part of the innate immune system but also serve to activate adaptive in response cancer. Real-time PCR; immunohistochemical stain and Western blotting analyses were performed clarify molecular alterations colorectal cancer (CRC) patients. We identified receptor 1 (TLR1), TLR2, TLR4 TLR8 gene expression levels downstream gene, i.e., interleukin-6 (IL-6), IL-8, interferon-α (IFN-α) myeloid differentiation primary-response protein-88...

Abstract Background Hericium erinaceus is an edible mushroom; its various pharmacological effects which have been investigated. This study aimed to demonstrate whether efficacy of oral administration H. mycelium (HEM) and isolated diterpenoid derivative, erinacine A, can act as anti-neuroinflammatory agent bring about neuroprotection using MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) mouse model Parkinson’s disease, results in motor disturbances, addition elucidating the mechanisms...

MicroRNA-29a is a key regulon that regulates hepatic stellate cells (HSCs) and mitigates liver fibrosis.However, the mechanism by which it does so remains largely undefined.The inhibition of bromodomain-4 protein (BRD4) represents novel therapeutic target in fibrosis.Therefore, purpose this study to investigate miR-29a regulation BRD4 signaling bile duct-ligation (BDL) animal model with regard developing cholestatic fibrosis.Hepatic tissue transgenic mice (miR-29aTg mice) displayed weak...

Arsenic trioxide (As2O3) exhibits promising anticarcinogenic activity in acute promyelocytic leukemic patients and induces apoptosis various tumor cells vitro. Here, we investigated the effect of natural alkaloid berberine on As2O3-mediated inhibition cancer cell migration using rat human glioma lines.The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to determine viability C6 U-87 after treatment with As2O3 or berberine, co-treatment berberine. The wound...

Abstract Adhesions are unavoidable consequences of surgery and other trauma. How to prevent the adhesions remains a big issue in healthcare system. The objective this study is test efficacy polycaprolactone (PCL) films as physical barriers reducing postoperative intra‐abdominal rat cecum‐abdominal wall model. PCL quite cheap compared with agents recently used market. fabrication method also very easy perform. Scanning electron microscope (SEM) showed multiple pores over upper bottom surfaces...

Objective Kawasaki disease (KD) is characterized by systemic vasculitis, and it the most common acquired heart in children. However, etiology immunopathogenesis of KD are still unclear. A genome‐wide association study (GWAS) identified polymorphisms CD40 , BLK FCGR2A as susceptibility genes for KD. No epigenetic array studies have previously been published. This was undertaken to investigate differences DNA methylation patients with compared controls. Methods The HumanMethylation27 BeadChip...

Kawasaki disease (KD) or syndrome is known as a vasculitis of small to medium-sized vessels, and coronary arteries are predominantly involved in childhood. Generally, 20–25% untreated with IVIG 3–5% treated KD patients have been developed artery lesions (CALs), such dilatation aneurysm. Understanding how aneurysms (CAAs) established maintained therefore great importance. Upon our previous genotyping data 157 valid subjects, genome-wide association study (GWAS) has conducted among 11 (7%)...

MicroRNA-29 (miR-29) has been found to reduce liver inflammation and fibrosis following a injury. Meanwhile, DNA methyltransferase reported participate in the development of non-alcoholic steatohepatitis (NASH). The aim this study is investigate miR-29a regulation signaling epigenetic program NASH progression. Methods: transgenic mice (miR-29aTg mice) wild-type littermates were subjected methionine-choline-deficient (MCD) diet-induced animal model NASH. Primary hepatic stellate cells...

In this study, high-performance indium-gallium-zinc oxide thin-film transistors (IGZO TFTs) with a dual-gate (DG) structure were manufactured using plasma treatment and rapid thermal annealing (RTA). Atomic force microscopy measurements showed that the surface roughness decreased upon increasing O

Melanin plays an important role in preventing ultraviolet (UV) light-induced skin damage. Overexposure to UV radiation can lead the formation of free radicals and trigger inflammation hyperpigmentation skin. Anthocyanin combat excessive body reduce occurrence inflammation. However, anthocyanin molecules are unstable highly susceptible degradation.The present study aims elucidate effects liposome-capsulated (LCA) from Hibiscus sabdariffa Linn. on melanogenesis human A375 melanocytes.The LCA...

Baicalein is the flavonoids with multiple pharmacological activities. The aim of our study was to investigate effects baicalein on colorectal cancer (CRC) and recognize targets treatment. To better understand baicalein's target, proteomic approaches were used purify identify protein substrates using 2D difference gel electrophoresis (2D SDS‐PAGE) elucidate proteins differential display. Results from this that treatment CRC cells results in reduced cell proliferation. As a result, displays...

Kawasaki disease (KD) is an acute febrile vasculitis in childhood, which the leading cause of acquired heart children. If untreated, KD can result coronary aneurysms 25% patients, and even under intravenous immunoglobulin (IVIG) treatment, 10-20% children will have IVIG resistance increased risk developing arteritis complication. Additional therapies should be explored to decrease incidence artery lesions improve prognosis KD. Autophagy has been reported play a critical role variety...

Background/Aims: Colorectal cancer (CRC) is the third most common type of and second leading cause cancer-related deaths worldwide. PRDXs are antioxidant enzymes that play an important role in cell differentiation, proliferation apoptosis have diverse functions malignancy development. However, mechanism aberrant overexpression PRDX6 CRC remains unclear. Methods: Boyden chamber assay, flow cytometry a lentiviral shRNA targeting transient transfection with pCMV-6-PRDX6 plasmid were used to...

Hericium erinaceus, a valuable pharmaceutical and edible mushroom, contains potent bioactive compounds such as H. erinaceus mycelium (HEM) its derived ethanol extraction of erinacine A, which have been found to regulate physiological functions in our previous study. However, HEM or A with post-treatment regimens also shows effects on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced neurotoxicity, but mechanisms remain unknown. By using annexin-V–fluorescein-isothiocyanate...

Hericium erinaceus, a consumable mushroom, has shown potential to enhance the production of neuroprotective bioactive metabolites. Traumatic brain injury (TBI) often leads cognitive, physical, and psychosocial impairments, resulting in neuroinflammation loss cortical neurons. In this research, effects H. erinaceus mycelium, its derivative erinacine C, along with underlying mechanisms, were examined terms oxidative stress modulation neurological improvement rat model mild traumatic (mTBI)....

Abstract The mechanism of homocysteine‐induced cell proliferation in human vascular smooth muscle cells (SMCs) remains unclear. We investigated the molecular mechanisms by which homocysteine affects expression cyclins A and D1 umbilical artery SMCs (HUASMCs). Homocysteine treatment induced HUASMCs increased levels D1. Knocking down either cyclin or small interfering RNA (siRNA) inhibited proliferation. Furthermore, with extracellular signal‐related kinase (ERK) inhibitor (PD98059) dominant...