A5102858775- Advanced Glycation End Products research
- Diet, Metabolism, and Disease
- Liver Disease Diagnosis and Treatment
- Alcohol Consumption and Health Effects
- DNA Repair Mechanisms
- RNA modifications and cancer
- Multiple and Secondary Primary Cancers
- Lipoproteins and Cardiovascular Health
- Cancer, Lipids, and Metabolism
- Anesthesia and Pain Management
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Carcinogens and Genotoxicity Assessment
- Lung Cancer Treatments and Mutations
- Plant tissue culture and regeneration
- Seed Germination and Physiology
- Cancer, Hypoxia, and Metabolism
- Cervical Cancer and HPV Research
- Genetic factors in colorectal cancer
- Cardiovascular Function and Risk Factors
- Peroxisome Proliferator-Activated Receptors
- Cardiac, Anesthesia and Surgical Outcomes
- Diabetic Foot Ulcer Assessment and Management
- Lipid metabolism and disorders
- Toxin Mechanisms and Immunotoxins
Kanazawa Medical University
2015-2024
Kanazawa Medical University Hospital
2024
Sapporo Medical University
2020-2023
Izumi City General Hospital
2022
Niigata University
2022
Creative Commons
2022
Okayama University
2001-2021
Tokyo Women's Medical University
2009-2021
Nara Institute of Science and Technology
2006-2020
National Hospital Organization
2018
The molecular role of the RecF protein in loading RecA onto single-stranded DNA (ssDNA)-binding protein-coated ssDNA has been obscured by facility with which RecO and RecR proteins alone perform this function. We now show that RecFOR RecOR define distinct functions operate optimally different contexts. RecFOR, but not RecOR, is most effective when RecF(R) bound near an ssDNA/double-stranded (dsDNA) junction. However, no enhanced binding affinity for such a are both required under all...
Reactive oxygen species (ROS) are potent oxidants that attack chromosomal DNA and free nucleotides, leading to oxidative damage causes genetic alterations. To avoid the ROS‐mediated mutagenesis, cells have elaborate mechanisms including powerful antioxidant components repair pathways eliminate damage. Because of effective anti‐mutagenic functions, it has been unclear what extent ROS contribute spontaneous mutagenesis. Here we show a significant portion mutations is actually caused by in...
The RecO and RecR proteins form a complex that promotes the nucleation of RecA protein filaments onto SSB protein-coated single-stranded DNA (ssDNA). However, even when are provided at optimal concentrations, loading is surprisingly slow, typically proceeding with lag 10 min or more. rate-limiting step in RecOR-promoted binding RecOR to ssDNA, which inhibited by despite documented interaction between SSB. Full activity seen only preincubated ssDNA prior addition slow SSB-coated involves C...
Abstract Aims/Introduction Uric acid is synthesized by oxidation of hypoxanthine and xanthine using a catalyzing enzyme, oxidoreductase (XOR), which can be source reactive oxygen species. Plasma XOR activity metabolic biomarker associated with obesity, hyperuricemia, liver dysfunction insulin resistance. However, it has recently been reported that in fat tissue low humans, unlike rodents, secreted from human tissue. Materials Methods The associations obesity hypoxanthine, plasma were...
The habitual excessive consumption of alcohol has been implicated in the onset and/or progression alcoholic-associated liver/brain diseases (ALD/ABD), lung disease, rheumatoid arthritis, and cardiac tissue injury. Alcohol (ethanol) is metabolized to acetaldehyde (AA), a two-carbon carbonyl compound that reacts with proteins form covalent AA-protein adducts (AAPAs). We herein propose AA liver brain generate AAPAs, which contribute alcohol-induced injury neurotoxicity vivo, respectively....
Head and neck squamous cell carcinoma (HNSCC) is a frequent malignancy with poor survival rate. Identifying the tumor suppressor gene (TSG) loci by genomic studies an important step to uncover molecular mechanisms involved in HNSCC pathogenesis. We therefore performed comprehensive analyses on loss of heterozygosity (LOH) using genome-wide panel 191 microsatellite markers 22 samples. found 53 significantly high LOH (>30%) 21 chromosomal arms; highest values those were observed 3p, 9p, 13q,...
Cardiovascular disease (CVD) is a lifestyle-related (LSRD) and one of the largest public health issues. Risk factors for CVD correlate with an excessive intake glucose and/or fructose, which has been shown to induce production advanced glycation end-products (AGEs). We previously identified AGEs derived from glyceraldehyde named them toxic (TAGE) due their cytotoxicities relationship LSRD. also reported that extracellular TAGE in vascular system may promote serum levels are associated risk...
Abstract Backgroud and Aim: Chronic hepatitis C virus (HCV) infection is a well known risk factor for hepatocellular carcinoma (HCC). The aim of this study to elucidate the genetic development recurrence HCC in patients with HCV. Methods: A total 468 HCV, including 265 were enrolled. We genotyped 88 single nucleotide polymorphisms (SNPs) 81 genes expected influence hepatocarcinogenesis using iPLEX assay. Risk was clarified by stratifying into groups based on multiplied odds ratio (MOR) SNPs...
Here, we provide evidence that YqjD, a hypothetical protein of Escherichia coli, is an inner membrane and ribosome binding protein. This expressed during the stationary growth phase, expression regulated by stress response sigma factor RpoS. YqjD possesses transmembrane motif in C-terminal region associates with 70S 100S ribosomes at N-terminal region. Interestingly, E. coli two paralogous proteins ElaB YgaM, which are bind to similar manner YqjD. Overexpression leads inhibition cell growth....
Hepatocyte cell death is a key feature of nonalcoholic steatohepatitis (NASH); however, the pathogenesis NASH currently remains unclear. We aimed to investigate effects intracellular glyceraldehyde (GA)-derived advanced glycation end-products (GA-AGEs) on human hepatocyte death. The accumulation GA-AGEs has been associated with induction DNA damage and necrotic Among GA-AGEs, caspase-3 identified as GA-AGE-modified protein abrogated function. Furthermore, activation apoptosis by...
Metabolic dysfunction-associated fatty liver disease (MAFLD), defined as hepatosteatosis with type 2 diabetes mellitus, overweight/obesity or metabolic dysregulation, has been proposed a new feature of chronic disease. Fatty acid-binding protein 4 (FABP4) is expressed in adipose tissue, and secreted FABP4 associated the development insulin resistance atherosclerosis. However, relationship between MAFLD not fully addressed.Associations markers, including FABP4, fibroblast growth factor 21...
Two independent cDNA clones encoding fructose 6-phosphate, 2-kinase/fructose 2,6-bisphosphatase were isolated from a human placental library. The deduced amino acid sequences showed that one of the clones, 2K-1, was almost identical to rat testis isozyme and other, 2K-3, different any known isozymes expressed in mammalian tissues. results Southern blot analysis suggested 2K-1 2K-3 encoded as single copy genes located parts genome. Since open reading frames not complete, we obtained 5'-end...